Mitophagy and Mitochondria Biogenesis Are Differentially Induced in Rat Skeletal Muscles during Immobilization and/or Remobilization
Abstract
:1. Introduction
2. Results
2.1. Mitochondria Biogenesis Adaptations during Muscle Immobilization and Remobilization Could Not Explain Changes in Mitochondria Abundance
2.2. Mitochondria Fusion and Fission Were Imbalanced in GA and TA Muscles during Immobilization and Remobilization
2.3. Mitochondria Trafficking along Microtubules Was Induced in Both Muscles during Immobilization and/or Remobilization
2.4. Parkin-Dependent and -Independent Mitophagy Was Activated in Muscles during Immobilization or Remobilization
2.5. Autophagy Was Induced and Sustained during Immobilization and Remobilization in the TA
2.6. Large and Sustained Activation of the CASA in the Immobilized and Remobilized TA
3. Discussion
4. Materials and Methods
4.1. Ethical Approval
4.2. Experimental Design
4.3. Bodyweight and Sample Preparation
4.4. Histological Analyses of Muscles
4.5. Quantitative Real-Time PCR Analysis
4.6. Protein Extraction
4.7. Citrate Synthase Activity
4.8. Western Blot Analysis
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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GA | TA | |
---|---|---|
Mass (g) | Mass (mg) | |
Con Imm Rem | 2.316 ± 0.053 1.751 ± 0.051 * 1.708 ± 0.049 * | 796 ± 20 656 ± 10 * 519 ± 20 *§ |
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Deval, C.; Calonne, J.; Coudy-Gandilhon, C.; Vazeille, E.; Bechet, D.; Polge, C.; Taillandier, D.; Attaix, D.; Combaret, L. Mitophagy and Mitochondria Biogenesis Are Differentially Induced in Rat Skeletal Muscles during Immobilization and/or Remobilization. Int. J. Mol. Sci. 2020, 21, 3691. https://doi.org/10.3390/ijms21103691
Deval C, Calonne J, Coudy-Gandilhon C, Vazeille E, Bechet D, Polge C, Taillandier D, Attaix D, Combaret L. Mitophagy and Mitochondria Biogenesis Are Differentially Induced in Rat Skeletal Muscles during Immobilization and/or Remobilization. International Journal of Molecular Sciences. 2020; 21(10):3691. https://doi.org/10.3390/ijms21103691
Chicago/Turabian StyleDeval, Christiane, Julie Calonne, Cécile Coudy-Gandilhon, Emilie Vazeille, Daniel Bechet, Cécile Polge, Daniel Taillandier, Didier Attaix, and Lydie Combaret. 2020. "Mitophagy and Mitochondria Biogenesis Are Differentially Induced in Rat Skeletal Muscles during Immobilization and/or Remobilization" International Journal of Molecular Sciences 21, no. 10: 3691. https://doi.org/10.3390/ijms21103691