Mouthwash-Based Highly Sensitive Pyro-Genotyping for Nine Sexually Transmitted Human Papilloma Virus Genotypes
Abstract
:1. Introduction
2. Results
2.1. Volume and Quality of Genomic DNA Recovered from Mouthwash
2.2. Amplification and Direct Sequencing of the 18 HPV Genotype-Harboring Plasmids from HeLa Cells
2.3. Amplification of Genes from HPV in the Clinical Mouthwash Waste Samples
2.4. Pyrosequencing Was Suitable for Genotyping Nine HPV Plasmids from HeLa Cells and Clinical Mouthwash Samples
2.5. Pyrosequencing Approach Was More Sensitive than the Conventional HPV-DNA Method
3. Discussion
4. Materials and Methods
4.1. Characteristics of the Patient Samples
4.2. Collection of Mouthwash
4.3. Construction of Plasmid DNAs Containing the Variable Sequence of the 18 HPV Genotypes
4.4. Direct Sequencing Using Plasmid DNAs Harboring the 18 HPV Genotypes from HeLa Cells
4.5. Quantitative Pyrosequencing Analysis Using Plasmid DNAs Harboring the 18 HPV Genotypes from HeLa Cells and Clinical Mouthwash Samples
4.6. HPV Detection using the Conventional HPV-DNA Method and Clinical Mouthwash Samples
4.7. HPV Detection using the Conventional HPV Genotyping Method and Clinical Mouthwash Samples
4.8. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
STI | Sexually transmitted infections |
HPV | Human papilloma virus |
Pyro-genotyping | Pyrosequencing-based genotyping |
HC2 | Hybrid capture 2 |
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Unit No. | DNA Conc. (ng) | Pyrosequencing | Restriction Enzyme-Based Genotyping | ||
---|---|---|---|---|---|
Amplification | HPV Genotype | Hybrid Capture | |||
1 | 811.9 | ||||
2 | 146.1 | Amplified | 18 | 18 | |
3 | 156.7 | ||||
4 | 708.7 | ||||
5 | 67.8 | ||||
6 | 117.2 | Amplified | 18 | Unidentified | |
7 | 275.5 | ||||
8 | 153.1 | ||||
9 | 456.2 | ||||
10 | 612.4 | ||||
11 | 104.5 | ||||
12 | 586.7 | ||||
13 | 367.6 | Amplified | 16 | Detected | 16 |
14 | 128.8 | Amplified | 18 | Unidentified | |
15 | 385.1 | ||||
16 | 327.5 | ||||
17 | 189.9 | ||||
18 | 252.4 | ||||
19 | 876.1 | ||||
20 | 8.4 | ||||
21 | 290.9 | ||||
22 | 37.4 | ||||
23 | 344.7 | Amplified | 11 | Unidentified | |
24 | 26.1 | ||||
25 | 344.8 | ||||
26 | 3.8 | ||||
27 | 0.9 | ||||
28 | 197.3 | ||||
29 | 431.3 | ||||
30 | 301.1 | ||||
31 | 280.6 | Amplified | 6 | Unidentified | |
32 | 275.2 | ||||
33 | 486.1 | ||||
34 | 380.2 | Amplified | 18 | Unidentified | |
35 | 298.4 | Amplified | 18 | Unidentified | |
36 | 260.3 | ||||
37 | 280.8 | ||||
38 | 180.2 | ||||
39 | 302.1 | ||||
40 | 197.6 | ||||
41 | 245.2 | ||||
42 | 266.1 | ||||
43 | 407.8 | ||||
44 | 28.1 | ||||
45 | 180.3 | ||||
46 | 199.1 | ||||
47 | 240.6 | Amplified | 18 | Unidentified | |
48 | 150.2 | Amplified | 18 | Unidentified | |
49 | 118.2 | ||||
50 | 260.8 | Amplified | 18 | Unidentified | |
51 | 110.8 | ||||
52 | 320.1 |
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Watanabe, Y.; Seto, Y.; Oikawa, R.; Nakazawa, T.; Furuya, H.; Matsui, H.; Hosono, S.; Noike, M.; Inoue, A.; Yamamoto, H.; et al. Mouthwash-Based Highly Sensitive Pyro-Genotyping for Nine Sexually Transmitted Human Papilloma Virus Genotypes. Int. J. Mol. Sci. 2020, 21, 3697. https://doi.org/10.3390/ijms21103697
Watanabe Y, Seto Y, Oikawa R, Nakazawa T, Furuya H, Matsui H, Hosono S, Noike M, Inoue A, Yamamoto H, et al. Mouthwash-Based Highly Sensitive Pyro-Genotyping for Nine Sexually Transmitted Human Papilloma Virus Genotypes. International Journal of Molecular Sciences. 2020; 21(10):3697. https://doi.org/10.3390/ijms21103697
Chicago/Turabian StyleWatanabe, Yoshiyuki, Yukiko Seto, Ritsuko Oikawa, Takara Nakazawa, Hanae Furuya, Hidehito Matsui, Sachiko Hosono, Mika Noike, Akiko Inoue, Hiroyuki Yamamoto, and et al. 2020. "Mouthwash-Based Highly Sensitive Pyro-Genotyping for Nine Sexually Transmitted Human Papilloma Virus Genotypes" International Journal of Molecular Sciences 21, no. 10: 3697. https://doi.org/10.3390/ijms21103697