SIRT7 Deacetylates STRAP to Regulate p53 Activity and Stability
Abstract
:1. Introduction
2. Results
2.1. SIRT7 Interacts with STRAP
2.2. STRAP Is Acetylated at Lysines 147, 148, and 156
2.3. STRAP Is Deacetylated by SIRT7
2.4. Acetylation of STRAP Regulates p53-Mediated Transcription
2.5. Acetylation of STRAP Modulates p53 Stability
2.6. STRAP Acetylation Levels Are Regulated by 5-FU
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Antibodies, Plasmids, and Small Interfering RNA
4.3. Co-Immunoprecipitation
4.4. GST Pull-Down Assay
4.5. Acetylation/Deacetylation Assay In Vivo
4.6. In Vitro Acetylation Assay
4.7. In Vitro Deacetylation Assay
4.8. Luciferase Reporter Assay
4.9. In Vivo Ubiquitination Assay
4.10. Immunofluorescence Staining
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Yu, M.; Shi, X.; Ren, M.; Liu, L.; Qi, H.; Zhang, C.; Zou, J.; Qiu, X.; Zhu, W.-G.; Zhang, Y.E.; et al. SIRT7 Deacetylates STRAP to Regulate p53 Activity and Stability. Int. J. Mol. Sci. 2020, 21, 4122. https://doi.org/10.3390/ijms21114122
Yu M, Shi X, Ren M, Liu L, Qi H, Zhang C, Zou J, Qiu X, Zhu W-G, Zhang YE, et al. SIRT7 Deacetylates STRAP to Regulate p53 Activity and Stability. International Journal of Molecular Sciences. 2020; 21(11):4122. https://doi.org/10.3390/ijms21114122
Chicago/Turabian StyleYu, Miao, Xiaoyan Shi, Mengmeng Ren, Lu Liu, Hao Qi, Chi Zhang, Junhua Zou, Xiaoyan Qiu, Wei-Guo Zhu, Ying E. Zhang, and et al. 2020. "SIRT7 Deacetylates STRAP to Regulate p53 Activity and Stability" International Journal of Molecular Sciences 21, no. 11: 4122. https://doi.org/10.3390/ijms21114122