A Flowable Placental Formulation Prevents Bleomycin-Induced Dermal Fibrosis in Aged Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Tissue Procurement and Ethics Statement
2.2. Tissue Processing and Cryopreservation
2.3. Assessment of FPF Cell Viability
2.4. In Vivo Testing
2.4.1. Animals
2.4.2. Dermal fibrosis model
2.5. Histological and Immunohistochemical Assessment
2.6. MicroRNA Array and Quantitative RT-PCR Analysis
2.6.1. Sample Preparation
2.6.2. Library Preparation and Sequencing (QIAseq miR Tissue)
2.6.3. RT2 qPCR
2.7. Statistical Ana Lysis
3. Results
3.1. Characterization of FPF
3.2. FPF Reduces Bleomycin-Induced Dermal Fibrosis in Aged Mouse Skin
3.3. FPF Treatment Downregulates the TGF-β Pathway
3.4. Infiltration of Inflammatory Cells Is Diminished in FPF-Treated Mice
3.5. FPF Decreases Fibroblast and Endothelial to Mesenchymal Transition in the Dermis
3.6. FPF Treatment Improves Transcriptional Dysregulation in Fibrosis Disease Pathogenesis
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Dhall, S.; Lerch, A.; Johnson, N.; Jacob, V.; Jones, B.; Park, M.S.; Sathyamoorthy, M. A Flowable Placental Formulation Prevents Bleomycin-Induced Dermal Fibrosis in Aged Mice. Int. J. Mol. Sci. 2020, 21, 4242. https://doi.org/10.3390/ijms21124242
Dhall S, Lerch A, Johnson N, Jacob V, Jones B, Park MS, Sathyamoorthy M. A Flowable Placental Formulation Prevents Bleomycin-Induced Dermal Fibrosis in Aged Mice. International Journal of Molecular Sciences. 2020; 21(12):4242. https://doi.org/10.3390/ijms21124242
Chicago/Turabian StyleDhall, Sandeep, Anne Lerch, Nicholas Johnson, Vimal Jacob, Brielle Jones, Min Sung Park, and Malathi Sathyamoorthy. 2020. "A Flowable Placental Formulation Prevents Bleomycin-Induced Dermal Fibrosis in Aged Mice" International Journal of Molecular Sciences 21, no. 12: 4242. https://doi.org/10.3390/ijms21124242