BMP-Induced MicroRNA-101 Expression Regulates Vascular Smooth Muscle Cell Migration
Abstract
:1. Introduction
2. Results
2.1. miR-101 Affects Functions of VSMCs
2.2. Expression of miR-101 is Induced by BMP Signaling
2.3. BMP Signaling Induces miR-101 Expression in a Smad-Dependent Manner
2.4. DOCK4 Is a Novel Target of miR-101
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Reverse Transcription Quatitative PCR (qRT-PCR)
4.3. miRNA Mimics and Anti-miRNA Oligonucleotides
4.4. RNA Interference
4.5. Immunoblotting
4.6. Cell Proliferation Assay
4.7. In Vitro Scratch Wound Assay
4.8. Luciferase Reporter Constructs
4.9. Luciferase Assay
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Park, N.; Kang, H. BMP-Induced MicroRNA-101 Expression Regulates Vascular Smooth Muscle Cell Migration. Int. J. Mol. Sci. 2020, 21, 4764. https://doi.org/10.3390/ijms21134764
Park N, Kang H. BMP-Induced MicroRNA-101 Expression Regulates Vascular Smooth Muscle Cell Migration. International Journal of Molecular Sciences. 2020; 21(13):4764. https://doi.org/10.3390/ijms21134764
Chicago/Turabian StylePark, Nanju, and Hara Kang. 2020. "BMP-Induced MicroRNA-101 Expression Regulates Vascular Smooth Muscle Cell Migration" International Journal of Molecular Sciences 21, no. 13: 4764. https://doi.org/10.3390/ijms21134764