Activation of NOTCH1 by Shear Force Elicits Immediate Cytokine Expression in Human Chondrocytes
Abstract
:1. Introduction
2. Results
2.1. Identification of Shear Stress Immediate Response Genes in Chondrocytes
2.2. Induction of Inflammatory Cytokines by NOTCH1
2.3. Effect of NOTCH1-Induced Cytokines on Osteoblast
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Treatment
4.2. RNA Sequencing
4.3. Quantitative RT-PCR (qRT-PCR)
4.4. Enzyme-Linked Immunosorbent Assay (ELISA)
4.5. Availability of Supporting Data
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Cheng, H.-J.; Hsu, W.-T.; Chen, C.-N.; Li, C. Activation of NOTCH1 by Shear Force Elicits Immediate Cytokine Expression in Human Chondrocytes. Int. J. Mol. Sci. 2020, 21, 4958. https://doi.org/10.3390/ijms21144958
Cheng H-J, Hsu W-T, Chen C-N, Li C. Activation of NOTCH1 by Shear Force Elicits Immediate Cytokine Expression in Human Chondrocytes. International Journal of Molecular Sciences. 2020; 21(14):4958. https://doi.org/10.3390/ijms21144958
Chicago/Turabian StyleCheng, Hao-Jen, Wan-Ting Hsu, Cheng-Nan Chen, and Chin Li. 2020. "Activation of NOTCH1 by Shear Force Elicits Immediate Cytokine Expression in Human Chondrocytes" International Journal of Molecular Sciences 21, no. 14: 4958. https://doi.org/10.3390/ijms21144958