Mast Cell β-Tryptase Is Enzymatically Stabilized by DNA
Abstract
:1. Introduction
2. Results
2.1. DNA Preserves the Enzymatic Activity of Tryptase
2.2. Single-Stranded DNA Is Not Capable of Preserving the Enzymatic Activity of Tryptase
2.3. Fragmented dsDNA Possesses Tryptase-Stabilizing Activity
2.4. Double-Stranded DNA Does Not Rescue the Enzymatic Activity of Inactivated Tryptase
2.5. DNA Promotes Histone Degradation by Tryptase
2.6. Tryptase Is Located in the Nucleus of Human Mast Cells
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. cDNA Preparation
4.3. Tryptase Activity
4.4. DNA Analysis
4.5. Histone Degradation
4.6. Purification and Culture of Human Skin Mast Cells
4.7. Immunofluorescence and Laser-Scanning Microscopy
4.8. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Alanazi, S.; Grujic, M.; Lampinen, M.; Rollman, O.; Sommerhoff, C.P.; Pejler, G.; Melo, F.R. Mast Cell β-Tryptase Is Enzymatically Stabilized by DNA. Int. J. Mol. Sci. 2020, 21, 5065. https://doi.org/10.3390/ijms21145065
Alanazi S, Grujic M, Lampinen M, Rollman O, Sommerhoff CP, Pejler G, Melo FR. Mast Cell β-Tryptase Is Enzymatically Stabilized by DNA. International Journal of Molecular Sciences. 2020; 21(14):5065. https://doi.org/10.3390/ijms21145065
Chicago/Turabian StyleAlanazi, Sultan, Mirjana Grujic, Maria Lampinen, Ola Rollman, Christian P. Sommerhoff, Gunnar Pejler, and Fabio Rabelo Melo. 2020. "Mast Cell β-Tryptase Is Enzymatically Stabilized by DNA" International Journal of Molecular Sciences 21, no. 14: 5065. https://doi.org/10.3390/ijms21145065