Identification of Long-Distance Transmissible mRNA between Scion and Rootstock in Cucurbit Seedling Heterografts
Abstract
:1. Introduction
2. Results
2.1. A High Number of Transcripts Directionally Movement in Grafted Cucumber and Pumpkin Seedlings
2.2. Function Characterization of Mobile mRNAs of Cucumber and Pumpkin Are Universal Common
2.3. Function Characterization of Mobile mRNAs of Cucumber and Pumpkin Are Individually Different
2.4. Identification of Long-Distance Trafficking Between Rootstock and Scion of Csa/Cmo mRNA
3. Discussion
4. Materials and Methods
4.1. Plant Materials, Growth Conditions, and Grafting
4.2. Genomic DNA Isolation and Genomic Purity Verification
4.3. RNA Isolation and cDNA Synthesis
4.4. Construction and Colony Sequencing
4.5. RNA and DNA Library Construction and Sequencing
4.6. Consensus Genome Construction
4.7. Mobile RNA Identification by RNA-seq
4.8. GO (Gene Ontology) Enrichment and KEGG (Kyoto Encyclopedia of Genes and Genomes) Pathway Analysis
4.9. Orthology Analysis
4.10. Quantitative RT-PCR
4.11. Data Statistical Analysis
4.12. Data Availability
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Liu, W.; Xiang, C.; Li, X.; Wang, T.; Lu, X.; Liu, Z.; Gao, L.; Zhang, W. Identification of Long-Distance Transmissible mRNA between Scion and Rootstock in Cucurbit Seedling Heterografts. Int. J. Mol. Sci. 2020, 21, 5253. https://doi.org/10.3390/ijms21155253
Liu W, Xiang C, Li X, Wang T, Lu X, Liu Z, Gao L, Zhang W. Identification of Long-Distance Transmissible mRNA between Scion and Rootstock in Cucurbit Seedling Heterografts. International Journal of Molecular Sciences. 2020; 21(15):5253. https://doi.org/10.3390/ijms21155253
Chicago/Turabian StyleLiu, Wenqian, Chenggang Xiang, Xiaojun Li, Tao Wang, Xiaohong Lu, Zixi Liu, Lihong Gao, and Wenna Zhang. 2020. "Identification of Long-Distance Transmissible mRNA between Scion and Rootstock in Cucurbit Seedling Heterografts" International Journal of Molecular Sciences 21, no. 15: 5253. https://doi.org/10.3390/ijms21155253