Ablation of Galectin-12 Inhibits Atherosclerosis through Enhancement of M2 Macrophage Polarization
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Animals and Animal Care
4.2. Preparation of Bone Marrow-Derived Macrophages
4.3. Cytokine Enzyme-Linked Immunosorbent Assay
4.4. Quantitative Polymerase Chain Reaction
4.5. Immunofluorescence Staining
4.6. Lipid Droplet Staining
4.7. Generating Doxycycline-Inducible Galectin-12 Knockdown THP-1 Cells
4.8. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
AcLDL | Acetylated LDL |
ABCA1 | ATP Binding Cassette Subfamily A Member 1 |
ABCG1 | ATP Binding Cassette Subfamily G Member 1 |
CRD | carbohydrate recognition domain |
BMDM | bone-marrow-derived macrophages |
CD36 | cluster of differentiation 36 |
cAMP | cyclic adenosine monophosphate |
DKO | galectin-12 and LDL receptor double knockout |
HCD | high-cholesterol diet |
Il-6 | interleukin-6 |
LPS | lipopolysaccharide |
LCD | low-cholesterol diet |
LDL | low-density lipoproteins |
LDLRKO | LDL receptor knockout |
MCP-1 | monocyte chemoattractant protein-1 |
OxLDL | oxidized LDL |
PMA | phorbol myristate acetate |
PKA | protein kinase A |
SRB1 | scavenger receptor class B type 1 |
TNF-α | tumor necrosis factor α |
DAPI | 4′,6-diamidino-2-phenylindole |
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Name | Sequence |
---|---|
138top | 5′-TGCTGACTCCTTGCAGCATGACCATCGTTTTGGCCACTGACTGACGATGGTCACTGCAAGGAGT-3′ |
138bottom | 5′-CCTGACTCCTTGCAGTGACCATCGTCAGTCAGTGGCCAAAACGATGGTCATGCTGCAAGGAGTC-3′ |
3991top | 5′-TGCTGAGAAAGTGCTGTCCATTCACAGTTTTGGCCACTGACTGACTGTGAATGCAGCACTTTCT-3′ |
3991bottom | 5′-CCTGAGAAAGTGCTGCATTCACAGTCAGTCAGTGGCCAAAACTGTGAATGGACAGCACTTTCTC-3′ |
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Lin, E.-S.; Hsu, Y.-A.; Chang, C.-Y.; Lin, H.-J.; Chen, C.S.; Wan, L. Ablation of Galectin-12 Inhibits Atherosclerosis through Enhancement of M2 Macrophage Polarization. Int. J. Mol. Sci. 2020, 21, 5511. https://doi.org/10.3390/ijms21155511
Lin E-S, Hsu Y-A, Chang C-Y, Lin H-J, Chen CS, Wan L. Ablation of Galectin-12 Inhibits Atherosclerosis through Enhancement of M2 Macrophage Polarization. International Journal of Molecular Sciences. 2020; 21(15):5511. https://doi.org/10.3390/ijms21155511
Chicago/Turabian StyleLin, En-Shyh, Yu-An Hsu, Ching-Yao Chang, Hui-Ju Lin, Chih Sheng Chen, and Lei Wan. 2020. "Ablation of Galectin-12 Inhibits Atherosclerosis through Enhancement of M2 Macrophage Polarization" International Journal of Molecular Sciences 21, no. 15: 5511. https://doi.org/10.3390/ijms21155511