Pyridoxine Deficiency Exacerbates Neuronal Damage after Ischemia by Increasing Oxidative Stress and Reduces Proliferating Cells and Neuroblasts in the Gerbil Hippocampus
Abstract
:1. Introduction
2. Results
2.1. Pyridoxine Deficiency Decreases PLP and Increases Homocysteine Levels in Serum
2.2. Pyridoxine Deficiency Causes Early Neuronal Death after Ischemia
2.3. Pyridoxine Deficiency Facilitates the Activation of Astrocytes and Microglia after Ischemia
2.4. Pyridoxine Deficiency Decreases Ischemia-Induced Proliferating Cells and Neuroblasts
2.5. Pyridoxine Deficiency Increases Lipid Peroxidation and Decreases Nrf2 and BDNF Expression
3. Discussion
4. Materials and Methods
4.1. Experimental Animals
4.2. Ischemic Surgery
4.3. Immunohistochemistry
4.4. High-Performance Liquid Chromatography (HPLC) Analysis
4.5. Malondialdehyde Assay
4.6. Western Blot Analysis
4.7. Data Analysis
4.8. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Jung, H.Y.; Kim, W.; Hahn, K.R.; Kang, M.S.; Kim, T.H.; Kwon, H.J.; Nam, S.M.; Chung, J.Y.; Choi, J.H.; Yoon, Y.S.; et al. Pyridoxine Deficiency Exacerbates Neuronal Damage after Ischemia by Increasing Oxidative Stress and Reduces Proliferating Cells and Neuroblasts in the Gerbil Hippocampus. Int. J. Mol. Sci. 2020, 21, 5551. https://doi.org/10.3390/ijms21155551
Jung HY, Kim W, Hahn KR, Kang MS, Kim TH, Kwon HJ, Nam SM, Chung JY, Choi JH, Yoon YS, et al. Pyridoxine Deficiency Exacerbates Neuronal Damage after Ischemia by Increasing Oxidative Stress and Reduces Proliferating Cells and Neuroblasts in the Gerbil Hippocampus. International Journal of Molecular Sciences. 2020; 21(15):5551. https://doi.org/10.3390/ijms21155551
Chicago/Turabian StyleJung, Hyo Young, Woosuk Kim, Kyu Ri Hahn, Min Soo Kang, Tae Hyeong Kim, Hyun Jung Kwon, Sung Min Nam, Jin Young Chung, Jung Hoon Choi, Yeo Sung Yoon, and et al. 2020. "Pyridoxine Deficiency Exacerbates Neuronal Damage after Ischemia by Increasing Oxidative Stress and Reduces Proliferating Cells and Neuroblasts in the Gerbil Hippocampus" International Journal of Molecular Sciences 21, no. 15: 5551. https://doi.org/10.3390/ijms21155551