An Inducible Diabetes Mellitus Murine Model Based on MafB Conditional Knockout under MafA-Deficient Condition
Abstract
:1. Introduction
2. Results
2.1. Validation of Cre Recombinase Efficiency in Pancreatic Islets Using R26GRR/Pdx1-CreERTM Mice
2.2. Assessment of Glucose Tolerance in A0BΔpanc and A0B2 Mice
2.3. Analysis of Metabolic Parameters in Urine and Blood
2.4. Histological Changes in Kidneys of Male A0BΔpanc Mice
2.5. Changes in the Histology of the Pancreas in A0BΔpanc Mice
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Generation of A0BΔpanc Mice
4.3. Assessment of Cre Activity Induced by Tamoxifen
4.4. Intraperitoneal Glucose Tolerance Test (IPGTT)
4.5. Biochemical Analysis of Blood and Urine
4.6. Kidney and Pancreas Pathology Analysis
4.7. IHC Analysis and Cell Counting
4.8. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
BB | Bio-breeding |
BUN | Blood urea nitrogen |
CRE | Serum creatinine |
DM | Diabetes mellitus |
EGFP | Enhanced green fluorescent protein |
H&E | Hematoxylin–eosin |
HFD | High-fat diet |
IHC | Immunohistochemistry |
IP | Intraperitoneal |
IPGTT | Intraperitoneal glucose tolerance test |
M&T | Masson trichrome |
NOD | Non-obese diabetic |
PBS | Phosphate-buffered saline |
PCR | Polymerase chain reaction |
PFA | Paraformaldehyde |
SD | Standard deviations |
SPF | Specific-pathogen-free |
STZ | Streptozocin |
T1D | Type 1 diabetes |
T2D | Type 2 diabetes |
TAM | Tamoxifen |
WT | Wild-type |
ZDF | Zucker diabetic fatty |
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Deng, Z.; Matsumoto, Y.; Kuno, A.; Ojima, M.; Xiafukaiti, G.; Takahashi, S. An Inducible Diabetes Mellitus Murine Model Based on MafB Conditional Knockout under MafA-Deficient Condition. Int. J. Mol. Sci. 2020, 21, 5606. https://doi.org/10.3390/ijms21165606
Deng Z, Matsumoto Y, Kuno A, Ojima M, Xiafukaiti G, Takahashi S. An Inducible Diabetes Mellitus Murine Model Based on MafB Conditional Knockout under MafA-Deficient Condition. International Journal of Molecular Sciences. 2020; 21(16):5606. https://doi.org/10.3390/ijms21165606
Chicago/Turabian StyleDeng, Zhaobin, Yuka Matsumoto, Akihiro Kuno, Masami Ojima, Gulibaikelamu Xiafukaiti, and Satoru Takahashi. 2020. "An Inducible Diabetes Mellitus Murine Model Based on MafB Conditional Knockout under MafA-Deficient Condition" International Journal of Molecular Sciences 21, no. 16: 5606. https://doi.org/10.3390/ijms21165606