Orchestration of Intracellular Circuits by G Protein-Coupled Receptor 39 for Hepatitis B Virus Proliferation
Abstract
:1. Introduction
2. Results
2.1. A Screen of GPCRs for HBV Replication
2.2. GPR39 Supports HBV Replication
2.3. GPR39 Activates HSR
2.4. GPR39-Targeted Hedgehog (HH) Signaling against HBV Replication
2.5. GPR39-Orchestrated Crosstalks among CEBPB, HSPs and HH Signaling for HBV
3. Discussion
4. Materials and Methods
4.1. Compounds and Cells
4.2. Plasmids
4.3. GPCR Pathway Analysis
4.4. Luciferase Assay
4.5. Quantitative Reverse Transcription-Polymerase Chain Reaction
4.6. Western Blot
4.7. Lentivirus
4.8. RNAi
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
HBV | hepatitis B virus |
HCC | hepatocellular carcinoma |
GPCR | G protein-coupled receptor |
HSP | heat shock protein |
CEBPB | CCAAT/enhancer binding protein-β |
HTA | host-targeting agent |
GPR39 | G protein-coupled receptor 39 |
NL | NanoLuc |
CRHR2 | corticotropin releasing hormone receptor 2 |
GPR88 | G protein-coupled receptor 88 |
hGPCRnet | human GPCR network |
GSK3B | glycogen synthase kinase 3β |
HSR | heat shock response |
GALR2 | galanin receptor 2 |
NPBWR1 | neuropeptides B and W receptor 1 |
MC4R | melanocortin-4 receptor |
CRE | cAMP response element |
NFAT-RE | nuclear factor of activated T-cells response element |
SRE | serum response element |
SRF-RE | serum response factor response element |
HSE | heat shock element |
Hdj1 | DnaJ heat shock protein family member B1 |
HSPA8 | heat shock 70 kD protein 8 |
QCN DH | quercetin dihydrate |
HH | hedgehog |
GLI | glioma-associated oncogene homologue |
SAG | smoothened agonist |
CyP | cyclophilin |
HSF | heat shock factor |
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Goto, K.; Nishitsuji, H.; Sugiyama, M.; Nishida, N.; Mizokami, M.; Shimotohno, K. Orchestration of Intracellular Circuits by G Protein-Coupled Receptor 39 for Hepatitis B Virus Proliferation. Int. J. Mol. Sci. 2020, 21, 5661. https://doi.org/10.3390/ijms21165661
Goto K, Nishitsuji H, Sugiyama M, Nishida N, Mizokami M, Shimotohno K. Orchestration of Intracellular Circuits by G Protein-Coupled Receptor 39 for Hepatitis B Virus Proliferation. International Journal of Molecular Sciences. 2020; 21(16):5661. https://doi.org/10.3390/ijms21165661
Chicago/Turabian StyleGoto, Kaku, Hironori Nishitsuji, Masaya Sugiyama, Nao Nishida, Masashi Mizokami, and Kunitada Shimotohno. 2020. "Orchestration of Intracellular Circuits by G Protein-Coupled Receptor 39 for Hepatitis B Virus Proliferation" International Journal of Molecular Sciences 21, no. 16: 5661. https://doi.org/10.3390/ijms21165661