Complex III Inhibition-Induced Pulmonary Hypertension Affects the Mitochondrial Proteomic Landscape
Abstract
:1. Introduction
2. Results
2.1. Complex III Inhibition Significantly Disrupted the Mitochondrial Proteome
2.2. Complex III Inhibition Significantly Dysregulated Mitochondrial Fatty Acid and TCA Metabolism
2.3. Complex III Inhibition Impaired Amino Acid Metabolism and Protein Repair
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Mitochondrial Isolation
4.3. Mass Spectrometry
4.3.1. In-Solution Tryptic Digestion of Isolated Mitochondria
4.3.2. Mass Spectrometry and Data Processing
4.3.3. Label-Free Quantitative Proteomics
4.4. Bioinformatics Analysis
4.5. Statistics
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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James, J.; Valuparampil Varghese, M.; Vasilyev, M.; Langlais, P.R.; Tofovic, S.P.; Rafikova, O.; Rafikov, R. Complex III Inhibition-Induced Pulmonary Hypertension Affects the Mitochondrial Proteomic Landscape. Int. J. Mol. Sci. 2020, 21, 5683. https://doi.org/10.3390/ijms21165683
James J, Valuparampil Varghese M, Vasilyev M, Langlais PR, Tofovic SP, Rafikova O, Rafikov R. Complex III Inhibition-Induced Pulmonary Hypertension Affects the Mitochondrial Proteomic Landscape. International Journal of Molecular Sciences. 2020; 21(16):5683. https://doi.org/10.3390/ijms21165683
Chicago/Turabian StyleJames, Joel, Mathews Valuparampil Varghese, Mikhail Vasilyev, Paul R. Langlais, Stevan P. Tofovic, Olga Rafikova, and Ruslan Rafikov. 2020. "Complex III Inhibition-Induced Pulmonary Hypertension Affects the Mitochondrial Proteomic Landscape" International Journal of Molecular Sciences 21, no. 16: 5683. https://doi.org/10.3390/ijms21165683