Photodynamic Activity of Tribenzoporphyrazines with Bulky Periphery against Wound Bacteria
Abstract
:1. Introduction
2. Results and Discussion
2.1. Spectral Properties
2.2. Singlet Oxygen Formation
2.3. Photostability
2.4. Photodynamic Activity against Bacteria
3. Materials and Methods
3.1. Materials
3.2. Spectral Properties
3.3. Singlet Oxygen Generation
3.4. Photostability Determination
3.5. Photodynamic Activity against Bacteria
3.5.1. Liposomes Preparation and Determination
3.5.2. Bacterial Strains and Culture Conditions
3.5.3. Photodynamic Activity
3.5.4. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Compound | Solvent | ΦFL | 106ΦP | ΦΔ | logε[nm] | |
---|---|---|---|---|---|---|
1 | DMF | 0.051 | 86.40 | 0.20 [26] | 4.49[346] | 4.67[688] |
DMSO | 0.029 | 37.60 | 0.27 | 4.45[346] | 4.67[691] | |
2 | DMF | 0.008 | 48.40 | 0.05 [26] | 4.69[346] | 4.83[690] |
DMSO | 0.002 | 4.84 | 0.08 | 4.65[346] | 4.84[693] | |
MgPc | DMF | 0.230 [46] | 327 [46] | 0.28 [46] | 4.91[346] | 5.36[670] |
DMSO | 0.181 | 4.77 | 0.14 | 4.80[346] | 5.33[673] | |
ZnPc | DMF | 0.200 [47] | 10.2 [48] | 0.56 [49] | - | |
DMSO | 0.170 [47] | 3.5 [48] | 0.67 [49] |
Compound | Mean Diameter [nm] | Dv10 [nm] | Dv50 [nm] | Dv90 [nm] |
---|---|---|---|---|
1 | 301 ± 3 | 126 | 217 | 629 |
2 | 188 ± 23 | 71 | 147 | 346 |
MgPc | 119 ± 4 | 65 | 115 | 171 |
Compound | 1 | 2 | MgPc |
---|---|---|---|
Concentration [M] | log reduction in bacterial growth | ||
Staphylococcus aureus | |||
10−4 | 3.5 ± 0.3 | 3.6 ± 0.2 | 4.9 ± 0.3 |
10−5 | >5.9 ± 0.0 | 4.3 ± 0.1 | 4.6 ± 0.2 |
MRSA | |||
10−4 | no activity | 0.7 ± 0.1 | >5.7 ± 0.1 |
10−5 | 5.1 ± 0.1 | 3.8 ± 0.0 | >5.7 ± 0.1 |
Staphylococcus epidermidis | |||
10−4 | 3.3 ± 0.2 | 3.6 ± 0.2 | >6.0 ± 0.0 |
10−5 | >5.7 ± 0.2 | >5.7 ± 0.3 | >5.7 ± 0.4 |
Streptococcus pyogenes | |||
10−4 | 0.5 ± 0.3 | 1.7 ± 0.2 | >4.7 ± 0.3 |
10−5 | >4.7 ± 0.3 | >4.7 ± 0.3 | >4.7 ± 0.3 |
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Stolarska, M.; Glowacka-Sobotta, A.; Mlynarczyk, D.T.; Dlugaszewska, J.; Goslinski, T.; Mielcarek, J.; Sobotta, L. Photodynamic Activity of Tribenzoporphyrazines with Bulky Periphery against Wound Bacteria. Int. J. Mol. Sci. 2020, 21, 6145. https://doi.org/10.3390/ijms21176145
Stolarska M, Glowacka-Sobotta A, Mlynarczyk DT, Dlugaszewska J, Goslinski T, Mielcarek J, Sobotta L. Photodynamic Activity of Tribenzoporphyrazines with Bulky Periphery against Wound Bacteria. International Journal of Molecular Sciences. 2020; 21(17):6145. https://doi.org/10.3390/ijms21176145
Chicago/Turabian StyleStolarska, Magdalena, Arleta Glowacka-Sobotta, Dariusz T. Mlynarczyk, Jolanta Dlugaszewska, Tomasz Goslinski, Jadwiga Mielcarek, and Lukasz Sobotta. 2020. "Photodynamic Activity of Tribenzoporphyrazines with Bulky Periphery against Wound Bacteria" International Journal of Molecular Sciences 21, no. 17: 6145. https://doi.org/10.3390/ijms21176145
APA StyleStolarska, M., Glowacka-Sobotta, A., Mlynarczyk, D. T., Dlugaszewska, J., Goslinski, T., Mielcarek, J., & Sobotta, L. (2020). Photodynamic Activity of Tribenzoporphyrazines with Bulky Periphery against Wound Bacteria. International Journal of Molecular Sciences, 21(17), 6145. https://doi.org/10.3390/ijms21176145