Next Article in Journal
T Cell Immunity to Bacterial Pathogens: Mechanisms of Immune Control and Bacterial Evasion
Previous Article in Journal
Bioactive Phenolics and Polyphenols: Current Advances and Future Trends
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Photodynamic Activity of Tribenzoporphyrazines with Bulky Periphery against Wound Bacteria

by
Magdalena Stolarska
1,
Arleta Glowacka-Sobotta
2,
Dariusz T. Mlynarczyk
3,
Jolanta Dlugaszewska
4,
Tomasz Goslinski
3,
Jadwiga Mielcarek
1 and
Lukasz Sobotta
1,*
1
Chair and Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznan, Poland
2
Chair and Department of Maxillofacial Orthopedics and Orthodontics, Poznan University of Medical Sciences, Bukowska 70, 60-812 Poznan, Poland
3
Chair and Department of Chemical Technology of Drugs, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznan, Poland
4
Chair and Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Sciences, Swiecickiego 4, 60-781 Poznan, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(17), 6145; https://doi.org/10.3390/ijms21176145
Submission received: 15 July 2020 / Revised: 21 August 2020 / Accepted: 24 August 2020 / Published: 26 August 2020
(This article belongs to the Section Molecular Biophysics)

Abstract

:
Magnesium(II) tribenzoporphyrazines with phenoxybutylsulfanyl substituents were evaluated as photosensitizers in terms of their optical properties against wound bacteria. In the UV-vis spectra of analyzed tribenzoporphyrazines, typical absorption ranges were found. However, the emission properties were very weak, with fluorescence quantum yields in the range of only 0.002–0.051. What is important, they revealed moderate abilities to form singlet oxygen with the quantum yields up to 0.27. Under irradiation, the macrocycles decomposed via photobleaching mechanism with the quantum yields up to 8.64 × 10−5. The photokilling potential of tribenzoporphyrazines was assessed against Streptococcus pyogenes, Staphylococcus epidermidis, as well as various strains of Staphylococcus aureus, including methicillin-sensitive and-resistant bacteria. Both evaluated photosensitizers revealed high photodynamic potential against studied bacteria (>3 logs). S. aureus growth was reduced by over 5.9 log, methicillin-resistant S. aureus by 5.1 log, S. epidermidis by over 5.7 log, and S. pyogenes by over 4.7 log.

1. Introduction

A wound is described as a break in the integrity and continuity of a tissue, mucus membrane, or an organ tissue [1]. After an injury, wounds heal themselves according to physiological mechanisms. However, chronic wounds can appear when the mechanism is impaired in elderly patients and patients suffering from diabetes, malnutrition, or cancer, where the immune system is not as efficient as usual [1,2]. Skin is an essential anatomical barrier that offers protection from pathogens. Therefore, open wounds are susceptible to the microbial environment and, as a consequence, potential microbial infections. Four phases of wound healing can be distinguished: hemostasis, inflammatory phase, proliferative, and remodeling [1,2,3]. These stages, while occurring consecutively, do have a partial overlap [4]. The inflammatory phase begins directly after the injury, and it aims to stop the spread of the lesion and contain the damage to a confined area [2,4]. During this stage, the neutrophils, monocytes, and later macrophages, regulated by cytokines, clean the wound on a tissue level to dispose of any foreign material, including microorganisms. Next, in the proliferative phase, collagen and other components of connective tissue extracellular matrix are produced, along with the rapid division of the cells and angiogenesis. The final phase, which takes place up to several weeks after the injury, is based on the modification of the newly-formed scar tissue, which takes over the functions of the tissue which existed before the trauma.
The most common infections found in lesions are caused by Staphylococcus aureus, including methicillin-resistant strains (MRSA), Streptococcus pyogenes, Staphylococcus epidermidis, and Pseudomonas aeruginosa [3,5,6,7,8]. The incidence of the bacterial contamination of wounds not only prolongs the healing process, but it is also associated with patient discomfort, an extension of hospital stay, and generally in a significant increase in treatment cost [9]. Although the influx of neutrophils in the inflammation stage is aimed to keep the wound free of bacteria [4], the infections often occur in patients suffering from other diseases, which impede the standard progress of wound healing [2].
Many classes of drugs and drug combinations accelerate wound healing, protect peri-wound areas from infections, and reduce the risk of further infection complications. Various antimicrobial pharmacological approaches have so far been considered, including topical formulations with silver ions, antibiotics, or iodine-containing compounds [2]. In many other antimicrobial studies, new achievements of nanotechnology, materials chemistry, and medicinal chemistry have been implemented. These include applications of nanoparticles [5,10], biomaterials and wound dressings [11], peptides [12], polymers [13], natural products [14], bioengineered tissue substitutes [15], as well as systems for photodynamic therapy (PDT) [16,17] and photothermal therapy [18]. A great concern is associated with the factors that impede the healing of wounds [3]. The main issue is related to the contamination of damaged tissue. It can not only slow down the process of wound healing but also might spread the infection to other parts of the body. Therefore the use of additional antimicrobial treatment approaches is greatly required [2,5].
Both the red and the near-infrared light reach the deepest parts of tissues treated with photodynamic therapy. However, broader applications of PDT are limited due to the low penetration of light through tissues [19]. Therefore, photodynamic therapy against microorganisms, also called photodynamic antimicrobial chemotherapy (PACT), offers its full potential for treatment and healing of superficial infections [16,17]. The irradiated photosensitizer mediates the generation of reactive oxygen species, which can act destructively on the cells in the vicinity. PACT offers advantages over other antimicrobial therapies, mostly due to stopping the ability of pathogens to develop specific resistance mechanisms [20]. This treatment approach has been successfully studied on an array of bacterial, fungal, and viral strains, among them also multi-resistant ones, with various photosensitizers, such as porphyrinoids, curcumin, dipyrromethene dyes or nanoparticles [21,22,23,24,25].
Herein we present the evaluation of the photokilling potential of tribenzoporphyrazines with bulky 4-(3,5-dihydroxymethylphenoxy)butylsulfanyl and 4-(3,5-dibutoxycarbonylphenoxy)butyl-sulfanyl moieties synthesized previously by our team [26] (Figure 1) against the wound bacteria. Studies were focused on the determination of the spectral properties, singlet oxygen formation evaluation, photostability evaluation as well as in vitro photocytotoxicity assessment against Streptococcus pyogenes, Staphylococcus epidermidis, and various methicillin-sensitive and -resistant Staphylococcus aureus strains.

2. Results and Discussion

2.1. Spectral Properties

Absorption spectra of porphyrinoids consist of two characteristic bands (Figure 2). The first, known as the Soret band, is located in the near-ultraviolet and the blue region of the visible spectrum. The second, called the Q band, appears in the low-energetic, red region of the visible spectrum [27,28,29,30]. The Soret band is a result of π-π* electron transitions upon light quant absorption and is related to movement of electrons from HOMO (a2u) orbital to the LUMO (eg) orbital [31,32]. Herein studied compounds revealed the Soret band in the wavelength range of 250–450 nm with both maxima appearing at ca. 346 nm in solution. The Q bands were noted in the range of 550–750 nm as the result of π-π* electron transitions from HOMO to LUMO. For the reference MgPc, the profile of the phthalocyanine spectrum was typical for a symmetrical molecule with an intense sharp unsplit band maximum at 670 nm in DMF resulting from the electron transfer from a1u to degenerated eg orbital [33,34]. Interestingly, the split of the Q band in 1 and 2 is a consequence of a reduction in symmetry from D4h observed for MgPc to C noted for both tribenzoporphyrazines. Also, a new component of the Q band was blue-shifted and revealed lower intensity than the Soret band. Moreover, flattening of the short wavelength component of the Q band in 1 in comparison with 2 was observed. It might be the result of the intramolecular coordination bond formed between an oxygen of the hydroxyl group present in the peripheral moiety and the central magnesium(II) ion. Recently, we reported that the appearance of intramolecular coordination is possible and that magnesium(II) ion creates relatively stable chelates [35]. Similarly to the report by Kobayashi [33], the long-wavelength component of the Q band of tribenzoporphyrazines 1 and 2 is ca. 30 nm red-shifted in comparison to the MgPc. It is related to the destabilization of molecular orbital in tribenzoporphyrazines. This phenomenon can also be responsible for lower intensities of the Q band [34]. Tribenzoporphyrazines 1 and 2 were compared to the previously reported porphyrazine I (Figure 3) [36]. An increase in the symmetry of I leads to the degeneration of eg orbital and is observed in the Q band as an unsplit sharp band. The molar absorption coefficients of MgPc (Table 1) indicate its high ability to absorb light. However, the decrease of symmetry and the introduction of sulfur atoms to the periphery of the molecule reduces logε to the value of 4.67 for 1. At ca. 500 nm, no band for 1 and 2 was observed as it had occurred in the case of I. The band at ca. 500 nm is a result of n-π* electron transfer from lone electron pair of the sulfur atom to the molecular orbital [37,38]. It seems that in the UV-vis spectra of studied tribenzoporphyrazines, the phenomenon of an n-π* electron transfer was negligible, whereas in the case of porphyrazine I, broadband with low intensity was observed [36]. Studied tribenzoporphyrazines presented a decreased ability of light absorption in comparison to MgPc (Table 1). Moreover, an increase in the Soret band intensities was noted in contrast to the Q bands for tribenzoporphyrazines (lgε[Q band]/lgε[Soret band] equal ca. 1.05, 1.04 and 1.11 for 1, 2 and MgPc, respectively).
Porphyrinoids easily emit fluorescence, and this phenomenon was also analyzed in the presented study. The researched tribenzoporphyrazines revealed only one emission band with the bathochromic shift in comparison to their absorption Q band (Figure 4). In contrast, sulfanylporphyrazine I, which is structurally related to 2, has shown dual emission. Dual emission is a result of two different excitation pathways. When the compound is excited with blue or ultraviolet light, electrons are transferred to the S2 state and rapidly come back to the ground state, simultaneously emitting light with the wavelength slightly higher than the absorption of the Soret band. If a compound is irradiated with red light (low energetic light), the electron transfer to the S1 state is observed, and the energy is lower than that for the transfer from the S2 state. The electrons returning to the ground state produce the red light emission at slightly higher wavelengths than Q band absorption [30,36]. The herein studied tribenzoporphyrazines 1 and 2, when excited with ultraviolet light, produced longwave emissions at the red region of the visible spectrum. It is caused by electron transformation and emission of light from the lowest excited state S1 upon irradiation with short-wavelength light. In the fluorescence spectra, minor band splitting was observed (Figure 4). A similar phenomenon has been reported by Harper and co-workers [39].
The highest quantum yield of fluorescence, equal to 0.230, was recorded for MgPc in DMF. This value was slightly higher than for ZnPc. Studied tribenzoporphyrazines 1 and 2 revealed fluorescence quantum yields values equal to 0.051 and 0.008, respectively. The introduction of the sulfur atom to 1 and 2 resulted in a decrease of the fluorescence, as reported before [40]. The lowered quantum yield values measured within studied sulfanyl tribenzoporphyrazines can be explained by the non-radiative electron transitions, the occurrence of which was reported by Ehrlich and co-workers [37]. Freyer and co-workers indicated that the extension of the macrocyclic ring from porphyrazine to tetrabenzoporphyrazine (phthalocyanine) increases fluorescence ability (ΦFL of porphyrazine 0.17; ΦFL of phthalocyanine 0.44) [41]. The same tendency was observed for I (0.005) [36], 2 (0.008) and MgPc (0.230).

2.2. Singlet Oxygen Formation

Singlet oxygen is considered as the primary bacteria-killing agent in PACT [42]. Macrocycle 1 was found to be the best singlet oxygen generator within the studied tribenzoporphyrazines (Table 1, Figure 5a). Kobayashi reported that the splitting of the Q band impacts the Φ value, and this feature can be used for the designing of macrocycles [33]. Symmetry is crucial for the comparison of 1, MgPc (Table 1), and previously reported compound I. In the case of I, no splitting in the Q band spectrum area was observed, and singlet oxygen quantum yield was equal 0.02 [36]. In comparison with MgPc and 1, for reference compound ZnPc, over 2-fold higher Φ value was noted. It is caused by the widely described in the literature “heavy atom effect” [30]. The expansion of the porphyrazine leads to different Φ values depending on the peripheral substituents. When benzene rings were fused with the magnesium(II) porphyrazine macrocycle, the value of singlet oxygen of 0.28 was noted. Interestingly, in the case of sulfanyl porphyrazine with eight isophthaloxybutyl substituents (I), the Φ value reaches 0.02 [36]. The exchange of three pairs of sulfanyl substituents of porphyrazine I for three fused benzene rings in the tribenzoporphyrazine 2 resulted in the increase of Φ value up to 0.05. Most of the sulfanyl porphyrazines studied in our group presented low singlet oxygen generation ability (Φ below 0.1), which could be linked with the impact of the sulfur atom on the macrocycle electronic structure [26,40,43,44]. Differently, compound 1 as a member of sulfanyl tribenzoporphyrazines reached Φ up to 0.27 in DMSO. This tendency can be explained by the possibility to form dimers by I and aggregates by tribenzoporphyrazines [36,45]. Higher singlet oxygen generation yields of 1 might be explained by the formation of the intramolecular complex between magnesium(II) ion and the oxygen of the hydroxyl group present in the peripheral substituents, which effectively block the π-π stacking interaction between molecules.

2.3. Photostability

Irradiation of porphyrinoids leads to the formation of low weight, colorless compounds, or other macrocyclic molecules, which is known as photobleaching or phototransformation, respectively [50]. Studied compounds irradiated with visible light underwent photobleaching processes as an irreversible decrease in the intensity of their absorption bands was observed (Figure 5b). The most unstable compound was MgPc in DMF with the photodecomposition quantum yield ΦP = 32.7 × 10−5, whereas the most photostable were MgPc and 2 dissolved in DMSO with the ΦP 4.77 × 10−6 and 4.84 × 10−6 respectively. This difference can be explained by the nature of applied solvent. It has previously been reported that DMSO and oxygen atoms easily coordinate with the magnesium(II) ion located in the center of the macrocyclic ring [35]. Kuznetsova and Kaliya associated coordination of DMSO to central metal ion to the lowest photodecomposition quantum yields [51]. Macrocycle 1 presented high ΦP in DMSO, which could be linked with relatively high singlet oxygen quantum yield of this compound (Table 1). Herein studied porphyrinoids, except MgPc, reveal photostability quantum yields at the 10−5 and 10−6 level, which allows for classifying them as stable photosensitizers. Dilber and co-workers have divided PSs into the stable and unstable group considering ΦP values [52]. Compounds with ΦP ca. 10−3 were categorized as labile, whereas those with ΦP at ca. 10−6 as stable. High lability of magnesium(II) phthalocyanine derivative was described by Łapok and co-workers as well [53]. Photoreactivity of magnesium(II) porphyrinoids, i.e., porphyrins, chlorophylls, and phthalocyanines, was reported by Sergeeva and Senge [54]. They explained this feature with the susceptibility of magnesium(II) macrocycles to the demetallation process.

2.4. Photodynamic Activity against Bacteria

The antibacterial photodynamic activity was assessed against Gram-positive bacteria derived from wound infections as nearly three-quarters of nosocomial infections have been related to surgical wounds [6]. Nowadays, the challenge posed for medical care is also in the treatment of chronic wounds, which consume 2–4% of the medical system finance of western countries [7]. The main Gram-positive bacteria in the infected wound development are Staphylococcus aureus, including MRSA and Streptococcus pyogenes [6,7,8].
The researched macrocycles were encapsulated in liposome vehicles as they present a hydrophobic nature. Liposomes were prepared using a thin-film hydration method. The positive charge, important to sufficient bacteria photoinactivation, was provided by a liposome ingredient—chloride salt of DOTAP. The size of the liposomes corresponded to earlier prepared formulations [55]. The average diameter of the obtained liposomes was measured at 301 nm, 188 nm, and 119 nm for 1, 2, and MgPc, respectively (Table 2). Liposome vehicles were used for solubilization of lipophilic photosensitizers enabling to keep them in the monomeric, non-aggregated forms, which is necessary for high photodynamic activity [56].
It should be underlined that all the studied compounds revealed high antibacterial activity with log reduction above three log—the FDA’s arbitrary threshold for bactericidal substances [42].
Statistically significant bacteria reduction for 1 was found at the concentration of 10−5 M (p = 0.02), for 2 at the concentration of 10−4 M (p = 0.04) and 10−5 M (p = 0.02) and for MgPc at 10−4 M and 10−5 M (p = 0.02).
The primary wound pathogen is S. aureus, responsible for the majority of human infections from mild to life-threatening, including bloodstream, skin, mucosa, soft tissues, and many other infections [57]. Among the studied macrocycles, tribenzoporphyrazine 1 presented the highest S. aureus killing rate of over 5.9 log at the concentration of 10−5 M, and under irradiation with a relatively low light dose of 30 J/cm2. Similar results of S. aureus photodynamic inactivation was reported before [56]. Interestingly, at higher concentrations, studied compounds revealed lower photodynamic bacterial growth reduction potential. This can be related to the π-π stacking phenomenon and aggregation. The obtained killing rate for 1 at 10−5 M concentration can be considered as total bacteria eradication. The same S. aureus photokilling level at similar dosimetry was reported by Masiera and co-workers, who noted 6 log (complete eradication) reduction in bacterial growth for unsubstituted porphycene (II, Figure 3) loaded into micelles at the concentration of 7 × 10−6 M [58]. Another macrocycle, phthalocyanine IV (Figure 3), presented higher activity at 10−4 M than at 10−5 M [59], which can be linked to a decreased tendency to form aggregates as the result of electrostatic repulsion between single molecules. The other main wound bacterium is methicillin-resistant Staphylococcus aureus (MRSA). This S. aureus strain appeared in the 1960s and still causes severe medical treatment issues up to the present day. This bacterial strain is still developing resistance to all known antibiotics. It is estimated that from 25 to 50% S. aureus hospital infections are in fact caused by the MRSA strains. Therefore, some institutions regard it as a top-priority drug-resistant microbe [57,60]. MRSA is also susceptible to the evaluated macrocycles. At 10−4 M concentration, tribenzoporphyrazines 1 and 2 presented no activity, whereas, at a 10-fold lower dose, a significant increase in photodynamic activity of up to 5.1 log was observed.
The third most common wound bacterium is Streptococcus pyogenes, which mainly causes mild superficial infections i.e., impetigo. However, rare but life-threatening incidents of superficial (skin, mucosa) infections are on the rise, including necrotizing fasciitis and Streptococcal toxic shock syndrome resulting in high mortality [8,61,62]. For the photodynamic treatment of S. pyogenes, a similar pattern as for MRSA was observed, but the log reduction of growth values was slightly lower (Table 3). The photodynamic elimination was examined not only against main wound microbes but also towards S. epidermidis—a skin microbiota species. This pathogen is especially dangerous for neonates, as it might cause sepsis, often leading to death [63,64]. Studied macrocycles revealed S. epidermidis photokilling rate at log values similar to those measured for S. aureus, up to over 5.7 log (10−5 M). Interestingly, macrocycle II loaded into pluronic micelles has reduced S. epidermidis colonies at the dose of 7 × 10−6 M and the light dose of only 6 J/cm2 [58]. A well-known photosensitizer, chlorin e6 (III, Figure 3), presented 6 log reduction in S. epidermidis growth when it was delivered at the same concentration (10−5 M), but using a 2-fold higher light dose (55 J/cm2) [65], in comparison to 1 and 2. Recently reported magnesium(II) phthalocyanine bearing eight positive charges at the periphery (IV) presented a high growth reduction of S. epidermidis of 5.42 log at the concentration of only 10−4 M. Interestingly, when IV was tested at 10−5 M concentration, bacteria reduction dropped to 2.48 log [59], which is lower than the FDA recommendation for bactericidal compounds [42]. Tribenzoporphyrazines 1 and 2 at 10−4 M showed lower photoactivity than at 10−5 M concentration. This might suggest that 10−5 M concentration is the limit for providing a monomeric form of the photosensitizers in POPC:DOTAP liposomes. Taking into account the results reported by Dlugaszewska and co-workers [59], and the outcomes obtained in this study, positively charged liposomes loaded with neutral photosensitizers provide at least the same levels of Gram-positive bacteria reduction when compared to positively charged photosensitizer molecules. The photocytotoxicity of tribenzoporphyrazines 1 and 2 against cancer cells was evaluated before. It was reported that among the studied liposomal formulations loaded with 1 and 2, POPC:DOTAP liposomes presented the lowest IC50 values against oral carcinoma cell lines (CAL-27, HSC-3) [45,55]. A simple topical administration route and an easy light delivery create perspectives for the effective treatment of bacterial infections associated with oral cancers in one medical procedure.

3. Materials and Methods

3.1. Materials

Studied compounds: 22,23-bis{4-[3,5-di(hydroxymethyl)phenoxy]butylsulfanyl}tribenzo[b,g,l]-porphyrazinato magnesium(II) (1) and 22,23-Bis [4-(3,5-dibutoxycarbonylphenoxy)butylsulfanyl]tri-benzo[b,g,l]porphyrazinato magnesium(II) (2) (Figure 1) were prepared according to earlier presented procedures [26]. The unsubstituted magnesium(II) phthalocyanine (MgPc) was purchased in Aldrich (Saint Louis, MO, USA). POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) and DOTAP (N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride) were purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA).

3.2. Spectral Properties

Absorption spectra and emission spectra of the studied compounds were recorded on a Shimadzu UV-160 spectrophotometer and a JASCO 6200 spectrofluorometer, respectively, in N,N-dimethylformamide (DMF) and dimethylsulfoxide (DMSO) solutions at ambient temperature [47,66,67].

3.3. Singlet Oxygen Generation

Singlet oxygen generation quantum yields were determined in DMF and DMSO solutions under the aerobic atmosphere at ambient temperature. Experiments were performed with a comparative method previously described [67]. DPBF (1,3-diphenylisobenzofuran, Aldrich, Saint Louis, MO, USA)) was used as a singlet oxygen chemical trap. The obtained kinetic parameters of DPBF decomposition were referred to unsubstituted zinc(II) phthalocyanine (Aldrich, Saint Louis, MO, USA)) with known singlet oxygen generation quantum yield values. The mixture of DPBF and studied macrocycle was irradiated with light at the Q band maximum. The preferred wavelength of light was separated with the monochromator (M250/1200/U with 2 nm/mm dispersion, Dk = 4 nm, Optel, Opole, Poland). A high-pressure xenon lamp with a broad emission spectrum (150 W, Optel, Opole, Poland) was used as a light source. In the determined time intervals, the UV-vis absorption spectra were recorded with a Shimadzu UV-160 spectrophotometer.

3.4. Photostability Determination

The photodegradation quantum yields were determined in DMF and DMSO under the aerobic atmosphere at ambient temperature following the method previously applied [68,69,70,71]. The high-pressure xenon lamp was used as a light source (150 W, Optel). The high energy light below 450 nm was cut off with the filter. Spectra were recorded at certain time intervals with a Shimadzu UV-160 spectrophotometer.

3.5. Photodynamic Activity against Bacteria

3.5.1. Liposomes Preparation and Determination

Liposomes were prepared with the thin-film hydration method followed by extrusion. POPC and DOTAP were mixed in a molar ratio of 8:2. Next, chloroform solutions containing 1, 2, and MgPc (1 mg/mL) were diluted to the final concentration of 200 µM in the vesicle. Then, the obtained mixture was evaporated under reduced pressure leading to a lipid film. Further, phosphate-buffered saline (PBS) was added and vortexed for 5 min. The unification process was performed by the extrusion of water mixture through the polycarbonate filter (200 nm). The size of the obtained vesicles was measured with NanoSight LM10 (Malvern Panalytical, Malvern, UK).

3.5.2. Bacterial Strains and Culture Conditions

The following wound Gram-positive bacteria were chosen: the clinical strains of methicillin-resistant Staphylococcus aureus (MRSA) and Streptococcus pyogenes, as well as ATCC collection strains of Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 49134). The clinical isolates were obtained from bacterial strains collection of the Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Sciences. The bacteria were cultured aerobically in Brain Heart Infusion (BHI) broth (Becton Dickinson, Franklin Lakes, NJ, USA) at 36 °C ± 1 °C for 20 h. Next, the bacterial cultures were centrifuged at 3000 rpm for 15 min, the supernatant was discarded, and the cells pellet was re-suspended and diluted in 10 mM PBS (pH = 7.0) to a final concentration of ca. 107 Colony Forming Units (CFU)/mL.

3.5.3. Photodynamic Activity

The photodynamic effect of photosensitizers (PSs) was evaluated by measuring the reduction in viability of microbial cells. Therefore, aliquots of the bacterial suspensions were placed in the 96-wells microplate. Next, the suspension of PS-loaded liposomes was added at appropriate volumes to achieve desired concentrations in the wells. The control samples were also prepared. The cultures were kept in the dark for 20 min (incubation time). Two sets of experiments were prepared. After incubation time one set was irradiated by the LED MultiChip Emitter consisting of 60 high-efficiency AlGaAs diode chips (Roithner LaserTechnik GmbH, Vienna, Austria) with maximum wavelength 690 nm, corresponding to the Q band of the PSs, at a fixed total light dose 30 J/cm2 (light phase). The dark phase was also evaluated. After incubation time (20 min), both light and dark phase cultures were transferred to the Tryptic Soy Agar (TSA, OXOID, Basingstoke, UK) plates (S. aureus, MRSA, S. epidermidis) and Columbia Agar with sheep blood (CBA, OXOID, UK) plates (S. pyogenes). After overnight incubation at 36 °C ± 1 °C (S. pyogenes was cultured in a CO2 enriched atmosphere), the CFUs were counted, and the number of viable microorganisms was calculated (number of CFU/mL). Next, the log reduction of living bacteria in each sample was determined. Experiments were performed in triplicates.

3.5.4. Statistical Analysis

The statistical analyses were performed using the STATISTICA software, v.13.0. The data for CFU/mL were converted to the logarithmic form. Pairwise comparisons were performed using the unpaired Student’s t, and U Mann-Whitney tests to determine whether a signifificant reduction in colony-forming units occurred as comparing irradiated to the non-irradiated samples. Data were analyzed for statistical signifificance with the Dunnett’s Multiple Comparison test. A probability value (p) of <0.05 was considered as signifificantly difffferent.

4. Conclusions

The optical properties and photocytotoxicities of two magnesium(II) tribenzoporphyrazines, bearing 4-(3,5-dihydroxymethylphenoxy)butylsulfanyl or 4-(3,5-dibutoxycarbonylphenoxy)butyl-sulfanyl substituents at the periphery, were studied. In the UV-vis absorption spectra, compounds in solutions showed the Soret bands in the wavelength range of 250–450 nm with the maxima at ca. 346 nm and the Q bands in the range of 550–750 nm. Interestingly, the spectra of both tribenzoporphyrazines were split in the Q band regions, and both molecules presented a decreased ability of light absorption in comparison to MgPc. Moreover, an increase of Soret bands intensity in comparison to Q bands for tribenzoporphyrazines was observed in comparison to MgPc (lgε[Q band]/lgε[Soret band] equal ca. 1.05, 1.04 and 1.11 for 1, 2 and MgPc respectively). Macrocycles 1 and 2 showed only one emission band shifted bathochromically when compared with the absorption Q band. In the fluorescence spectra, minor band splitting was observed. The highest quantum yield of fluorescence was recorded for MgPc in DMF, equal 0.28. The studied tribenzoporphyrazines presented fluorescence quantum yield values of 0.051 and 0.008 for 1 and 2, respectively. Within the studied tribenzoporphyrazines, macrocycle 1 was found to be the best singlet oxygen generator. The reference ZnPc presented over 2-fold higher Φ value than MgPc and 1. Noteworthy is the fact that macrocycle 1 as a member of sulfanyl tribenzoporphyrazines reached Φ up to 0.27 in DMSO in comparison to other sulfanyl porphyrazines for which the Φ usually does not exceed 0.1. The studied compounds underwent photobleaching processes when irradiated with visible light, and the ratios of the processes were linked to the used solvent. The most unstable compound was MgPc in DMF with the photodecomposition quantum yield ΦP equal 32.7 × 10−5, whereas the most photostable were MgPc and 2 with ΦP equal 4.77 × 10−6 and 4.84 × 10−6 in DMSO solutions, respectively. Contrarily, 1 in DMSO presented high ΦP, which could be related to its relatively high singlet oxygen quantum yield in this solvent. Both tribenzoporphyrazines revealed photostability quantum yield values spanning between 10−5 and 10−6, which allows to classify them as stable photosensitizers.
Antibacterial photodynamic activity was assessed against Gram-positive bacteria derived from the wound infections Staphylococcus aureus, including MRSA and Streptococcus pyogenes. Due to the hydrophobic character of the studied compounds, they were encapsulated into liposome vehicles. Liposomes were obtained with an average diameter equal 301 nm, 188 nm, and 119 nm for 1, 2, and MgPc, respectively. Among the studied macrocycles, tribenzoporphyrazine 1 revealed the highest S. aureus killing rate of over 5.9 log at the concentration of 10−5 M. Its activity was observed under irradiation with a relatively low light dose of 30 J/cm2. When evaluated against MRSA, tribenzoporphyrazines 1 and 2 were not active at the concentration of 10−4 M, whereas, at a 10-fold lower dose, their significant increase in photocytotoxicity, of up to 5.1 log reduction was noted. Similar regularity was observed for S. pyogenes, but the log reduction values were somewhat lower. Tested macrocycles also revealed photokilling of S. epidermidis, naturally occurring on skin, at log values similar to those observed for S. aureus up to over 5.7 (10−5 M). According to the herein presented data, positively charged liposomes loaded with non-charged photosensitizers provide similar efficacy for Gram-positive bacteria photokilling potential as positively charged molecules of photosensitizers.

Author Contributions

Conceptualization, M.S. and L.S.; Data curation, M.S., A.G.-S., D.T.M. and J.D.; Formal analysis, M.S., A.G.-S. and J.D.; Funding acquisition, T.G.; Investigation, M.S.; Methodology, M.S.; Project administration, L.S.; Supervision, L.S.; Writing—original draft, M.S., A.G.-S., D.T.M. and L.S.; Writing—review & editing, T.G., J.M. and L.S. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the National Science Centre, Poland, grant number 2016/21/B/NZ9/00783.

Conflicts of Interest

Authors declare no conflict of interest.

References

  1. Singh, S.; Young, A.; McNaught, C.-E. The physiology of wound healing. Surgery 2017, 35, 473–477. [Google Scholar] [CrossRef]
  2. Han, G.; Ceilley, R. Chronic Wound Healing: A Review of Current Management and Treatments. Adv. Ther. 2017, 34, 599–610. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  3. Guo, S.; DiPietro, L.A. Factors Affecting Wound Healing. J. Dent. Res. 2010, 89, 219–229. [Google Scholar] [CrossRef] [PubMed]
  4. Ana Cristina de Oliveira, G.; Costa, T.F.; De Araújo Andrade, Z.; Medrado, A.R.A.P. Wound healing—A literature review. An. Bras. Dermatol. 2016, 91, 614–620. [Google Scholar] [CrossRef] [Green Version]
  5. Permana, A.D.; Mir, M.; Utomo, E.; Donnelly, R.F. Bacterially sensitive nanoparticle-based dissolving microneedles of doxycycline for enhanced treatment of bacterial biofilm skin infection: A proof of concept study. Int. J. Pharm. X 2020, 2, 100047. [Google Scholar] [CrossRef]
  6. Dai, T.; Huang, Y.-Y.; Hamblin, M.R. Photodynamic therapy for localized infections—State of the art. Photodiagn. Photodyn. Ther. 2009, 6, 170–188. [Google Scholar] [CrossRef] [Green Version]
  7. Drago, F.; Gariazzo, L.; Cioni, M.; Trave, I.; Parodi, A. The microbiome and its relevance in complex wounds. Eur. J. Dermatol. 2019, 29, 6–13. [Google Scholar] [CrossRef]
  8. Cardona, A.F.; Wilson, S.E. Skin and Soft-Tissue Infections: A Critical Review and the Role of Telavancin in Their Treatment. Clin. Infect. Dis. 2015, 61, S69–S78. [Google Scholar] [CrossRef] [Green Version]
  9. Collier, M. Recognition and management of wound infections. World Wide Wounds 2004, 7, 8–14. [Google Scholar]
  10. Abazari, M.; Ghaffari, A.; Rashidzadeh, H.; Momeni badeleh, S.; Maleki, Y. Current status and future outlook of nano--based systems for burn wound management. J. Biomed. Mater. Res. 2020, 108, 1934–1952. [Google Scholar] [CrossRef]
  11. Shariati, A.; Moradabadi, A.; Azimi, T.; Ghaznavi-Rad, E. Wound healing properties and antimicrobial activity of platelet-derived biomaterials. Sci. Rep. 2020, 10, 1–9. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  12. Sonamuthu, J.; Cai, Y.; Liu, H.; Kasim, M.S.M.; Vasanthakumar, V.R.; Pandi, B.; Wang, H.; Yao, J. MMP-9 responsive dipeptide-tempted natural protein hydrogel-based wound dressings for accelerated healing action of infected diabetic wound. Int. J. Biol. Macromol. 2020, 153, 1058–1069. [Google Scholar] [CrossRef] [PubMed]
  13. Sun, A.; He, X.; Li, L.; Li, T.; Liu, Q.; Zhou, X.; Ji, X.; Li, W.; Qian, Z. An injectable photopolymerized hydrogel with antimicrobial and biocompatible properties for infected skin regeneration. NPG Asia Mater. 2020, 12, 1–11. [Google Scholar] [CrossRef]
  14. Yang, W.-T.; Ke, C.-Y.; Wu, W.-T.; Tseng, Y.-H.; Lee, R.-P. Antimicrobial and anti-inflammatory potential of Angelica dahurica and Rheum officinale extract accelerates wound healing in Staphylococcus aureus-infected wounds. Sci. Rep. 2020, 10, 1–10. [Google Scholar] [CrossRef] [Green Version]
  15. Pereira, R.F.; Bártolo, P.J. Traditional Therapies for Skin Wound Healing. Adv. Wound Care 2016, 5, 208–229. [Google Scholar] [CrossRef] [Green Version]
  16. Oyama, J.; Fernandes Herculano Ramos-Milaré, Á.C.; Lopes Lera-Nonose, D.S.S.; Nesi-Reis, V.; Galhardo Demarchi, I.; Alessi Aristides, S.M.; Juarez Vieira Teixeira, J.; Gomes Verzignassi Silveira, T.; Campana Lonardoni, M.V. Photodynamic therapy in wound healing in vivo: A systematic review. Photodiagn. Photodyn. Ther. 2020, 30, 101682. [Google Scholar] [CrossRef]
  17. Nesi-Reis, V.; Lera-Nonose, D.S.S.L.; Oyama, J.; Silva-Lalucci, M.P.P.; Demarchi, I.G.; Aristides, S.M.A.; Teixeira, J.J.V.; Silveira, T.G.V.; Lonardoni, M.V.C. Contribution of photodynamic therapy in wound healing: A systematic review. Photodiagn. Photodyn. Ther. 2018, 21, 294–305. [Google Scholar] [CrossRef]
  18. Wang, H.; Zhao, B.; Dong, W.; Zhong, Y.; Zhang, X.; Gong, Y.; Zhan, R.; Xing, M.; Zhang, J.; Luo, G.; et al. A dual-targeted platform based on graphene for synergistic chemo-photothermal therapy against multidrug-resistant Gram-negative bacteria and their biofilms. Chem. Eng. J. 2020, 393, 124595. [Google Scholar] [CrossRef]
  19. Avci, P.; Gupta, A.; Sadasivam, M.; Vecchio, D.; Pam, Z.; Pam, N.; Hamblin, M.R. Low-level laser (light) therapy (LLLT) in skin: Stimulating, healing, restoring. Semin. Cutan. Med. Surg. 2013, 32, 41–52. [Google Scholar]
  20. Tavares, A.; Carvalho, C.M.B.; Faustino, M.A.; Neves, M.G.P.M.S.; Tomé, J.P.C.; Tomé, A.C.; Cavaleiro, J.A.S.; Cunha, Â.; Gomes, N.C.M.; Alves, E.; et al. Antimicrobial Photodynamic Therapy: Study of Bacterial Recovery Viability and Potential Development of Resistance after Treatment. Mar. Drugs 2010, 8, 91–105. [Google Scholar] [CrossRef] [Green Version]
  21. Carpenter, B.L.; Situ, X.; Scholle, F.; Bartelmess, J.; Weare, W.W.; Ghiladi, R.A. Antiviral, Antifungal and Antibacterial Activities of a BODIPY-Based Photosensitizer. Molecules 2015, 20, 10604–10621. [Google Scholar] [CrossRef] [Green Version]
  22. Piskorz, J.; Dlugaszewska, J.; Porolnik, W.; Teubert, A.; Mielcarek, J. Boron-dipyrromethene derivatives bearing N-alkyl phthalimide and amine substituents of potential application in the photoinactivation of bacteria. Dyes Pigments 2020, 178, 108322. [Google Scholar] [CrossRef]
  23. Kumar, P.S.M.; Francis, A.P.; Devasena, T. Biosynthesized and Chemically Synthesized Titania Nanoparticles: Comparative Analysis of Antibacterial Activity. J. Environ. Nanotechnol. 2014, 3, 73–81. [Google Scholar] [CrossRef] [Green Version]
  24. Hamidi, A.; Hassani, L.; Mohammadi, F.; Jahangoshayi, P.; Mohammadi, K. The biological effects of vanadyl curcumin and vanadyl diacetylcurcumin complexes: The effect on structure, function and oxidative stability of the peroxidase enzyme, antibacterial activity and cytotoxic effect. J. Enzyme Inhib. Med. Chem. 2016, 31, 1124–1131. [Google Scholar] [CrossRef] [PubMed]
  25. Elashnikov, R.; Radocha, M.; Panov, I.; Rimpelova, S.; Ulbrich, P.; Michalcova, A.; Svorcik, V.; Lyutakov, O. Porphyrin-silver nanoparticles hybrids: Synthesis, characterization and antibacterial activity. Mater. Sci. Eng. C 2019, 102, 192–199. [Google Scholar] [CrossRef] [PubMed]
  26. Mlynarczyk, D.; Lijewski, S.; Falkowski, M.; Piskorz, J.; Szczolko, W.; Sobotta, L.; Stolarska, M.; Popenda, L.; Jurga, S.; Konopka, K.; et al. Dendrimeric sulfanyl porphyrazines: Synthesis, physico-chemical characterization and biological activity for potential applications in anticancer photodynamic therapy. ChemPlusChem 2016, 81, 460. [Google Scholar] [CrossRef] [PubMed]
  27. Günsel, A.; Güzel, E.; Bilgiçli, A.T.; Atmaca, G.Y.; Erdoğmuş, A.; Yarasir, M.N. Synthesis and investigation of photophysicochemical properties of novel ketone-substituted gallium (III) and indium (III) phthalocyanines with high singlet oxygen yield for photodynamic therapy. J. Lumin. 2017, 192, 888–892. [Google Scholar] [CrossRef]
  28. Wierzchowski, M.; Łażewski, D.; Tardowski, T.; Grochocka, M.; Czajkowski, R.; Sobiak, S.; Sobotta, L. Nanomolar photodynamic activity of porphyrins bearing 1,4,7-trioxanonyl and 2-methyl-5-nitroimidazole moieties against cancer cells. J. Photochem. Photobiol. B Biol. 2020, 202, 111703. [Google Scholar] [CrossRef]
  29. Sobotta, L.; Ziental, D.; Sniechowska, J.; Dlugaszewska, J.; Potrzebowski, M.J. Lipid vesicle-loaded meso-substituted chlorins of high in vitro antimicrobial photodynamic activity. Photochem. Photobiol. Sci. 2019, 18, 213–223. [Google Scholar] [CrossRef]
  30. Sobotta, L.; Dlugaszewska, J.; Ziental, D.; Szczolko, W.; Koczorowski, T.; Goslinski, T.; Mielcarek, J. Optical properties of a series of pyrrolyl-substituted porphyrazines and their photoinactivation potential against Enterococcus faecalis after incorporation into liposomes. J. Photochem. Photobiol. A Chem. 2019, 368, 104–109. [Google Scholar] [CrossRef]
  31. Kandaz, M.; Özkaya, A.R.; Koca, A.; Salih, B. Water and alcohol-soluble octakis-metalloporphyrazines bearing sulfanyl polyetherol substituents: Synthesis, spectroscopy and electrochemistry. Dyes Pigments 2007, 74, 483–489. [Google Scholar] [CrossRef]
  32. Michel, S.L.J.; Hoffman, B.M.; Baum, S.M.; Barrett, A.G.M. Peripherally Functionalized Porphyrazines: Novel Metallomacrocycles with Broad, Untapped Potential. In Progress in Inorganic Chemistry; Karlin, K.D., Ed.; John Wiley & Sons, Inc.: Hoboken, NJ, USA, 2001; pp. 473–590. ISBN 978-0-471-22711-3. [Google Scholar]
  33. Kobayashi, N. Spectroscopically and/or Structurally Intriguing Phthalocyanines and Related Compounds. Part 1. Monomeric systems. IVUZKKT 2019, 62, 4–46. [Google Scholar] [CrossRef]
  34. Mack, J.; Kobayashi, N. Low Symmetry Phthalocyanines and Their Analogues. Chem. Rev. 2011, 111, 281–321. [Google Scholar] [CrossRef] [PubMed]
  35. Kasprzycki, P.; Sobotta, L.; Lijewski, S.; Wierzchowski, M.; Goslinski, T.; Mielcarek, J.; Radzewicz, C.; Fita, P. Unusual cis-diprotonated forms and fluorescent aggregates of non-peripherally alkoxy-substituted metallophthalocyanines. Phys. Chem. Chem. Phys. 2017, 19, 21390–21400. [Google Scholar] [CrossRef] [PubMed]
  36. Lijewski, S.; Gierszewski, M.; Sobotta, L.; Piskorz, J.; Kordas, P.; Kucinska, M.; Baranowski, D.; Gdaniec, Z.; Murias, M.; Karolczak, J.; et al. Photophysical properties and photochemistry of a sulfanyl porphyrazine bearing isophthaloxybutyl substituents. Dyes Pigments 2015, 113, 702–708. [Google Scholar] [CrossRef]
  37. Ehrlich, L.A.; Skrdla, P.J.; Jarrell, W.K.; Sibert, J.W.; Armstrong, N.R.; Saavedra, S.S.; Barrett, A.G.M.; Hoffman, B.M. Preparation of Polyetherol-Appended Sulfur Porphyrazines and Investigations of Peripheral Metal Ion Binding in Polar Solvents. Inorg. Chem. 2000, 39, 3963–3969. [Google Scholar] [CrossRef]
  38. Baygu, Y.; Yıldız, B.; Kabay, N.; Gök, Y. Novel magnesium and zinc porphyrazines containing galactose moieties: Synthesis via click reaction and characterization. Inorg. Chem. Commun. 2016, 71, 35–40. [Google Scholar] [CrossRef]
  39. Harper, S.R.; Pfrunder, M.C.; Esdaile, L.J.; Jensen, P.; McMurtrie, J.C.; Arnold, D.P. Synthetic, Structural, and Spectroscopic Studies of Bis(porphyrinzinc) Complexes Linked by Two-Atom Conjugating Bridges: Bis(porphyrinzinc) Complexes Linked by Two-Atom Bridges. Eur. J. Org. Chem. 2015, 2015, 2807–2825. [Google Scholar] [CrossRef]
  40. Gierszewski, M.; Falkowski, M.; Sobotta, L.; Stolarska, M.; Popenda, L.; Lijewski, S.; Wicher, B.; Burdzinski, G.; Karolczak, J.; Jurga, S.; et al. Porphyrazines with peripheral isophthaloxyalkylsulfanyl substituents and their optical properties. J. Photochem. Photobiol. A Chem. 2015, 307, 54–67. [Google Scholar] [CrossRef]
  41. Freyer, W.; Mueller, S.; Teuchner, K. Photophysical properties of benzoannelated metal-free phthalocyanines. J. Photochem. Photobiol. A Chem. 2004, 163, 231–240. [Google Scholar] [CrossRef]
  42. Sobotta, L.; Skupin-Mrugalska, P.; Piskorz, J.; Mielcarek, J. Porphyrinoid photosensitizers mediated photodynamic inactivation against bacteria. Eur. J. Med. Chem. 2019, 175, 72–106. [Google Scholar] [CrossRef] [PubMed]
  43. Piskorz, J.; Lijewski, S.; Gierszewski, M.; Gorniak, K.; Sobotta, L.; Wicher, B.; Tykarska, E.; Düzgüneş, N.; Konopka, K.; Sikorski, M.; et al. Sulfanyl porphyrazines: Molecular barrel-like self-assembly in crystals, optical properties and in vitro photodynamic activity towards cancer cells. Dyes Pigments 2016, 136, 898–908. [Google Scholar] [CrossRef]
  44. Piskorz, J.; Skupin, P.; Lijewski, S.; Korpusinski, M.; Sciepura, M.; Konopka, K.; Sobiak, S.; Goslinski, T.; Mielcarek, J. Synthesis, physical–chemical properties and in vitro photodynamic activity against oral cancer cells of novel porphyrazines possessing fluoroalkylthio and dietherthio substituents. J. Fluor. Chem. 2012, 135, 265–271. [Google Scholar] [CrossRef]
  45. Mlynarczyk, D.T.; Piskorz, J.; Popenda, L.; Stolarska, M.; Szczolko, W.; Konopka, K.; Jurga, S.; Sobotta, L.; Mielcarek, J.; Düzgüneş, N.; et al. S-seco-porphyrazine as a new member of the seco-porphyrazine family—Synthesis, characterization and photocytotoxicity against cancer cells. Bioorg. Chem. 2020, 96, 103634. [Google Scholar] [CrossRef]
  46. Taştemel, A.; Karaca, B.Y.; Durmuş, M.; Bulut, M. Photophysical and photochemical properties of novel metallophthalocyanines bearing 7-oxy-3-( m -methoxyphenyl)coumarin groups. J. Lumin. 2015, 168, 163–171. [Google Scholar] [CrossRef]
  47. Ogunsipe, A.; Maree, D.; Nyokong, T. Solvent effects on the photochemical and fluorescence properties of zinc phthalocyanine derivatives. J. Mol. Struct. 2003, 650, 131–140. [Google Scholar] [CrossRef] [Green Version]
  48. Sobotta, L.; Lijewski, S.; Dlugaszewska, J.; Nowicka, J.; Mielcarek, J.; Goslinski, T. Photodynamic inactivation of Enterococcus faecalis by conjugates of zinc(II) phthalocyanines with thymol and carvacrol loaded into lipid vesicles. Inorg. Chim. Acta 2019, 489, 180–190. [Google Scholar] [CrossRef]
  49. Ogunsipe, A.; Durmuş, M.; Atilla, D.; Gürek, A.G.; Ahsen, V.; Nyokong, T. Synthesis, photophysical and photochemical studies on long chain zinc phthalocyanine derivatives. Synth. Met. 2008, 158, 839–847. [Google Scholar] [CrossRef]
  50. Bonnett, R.; Martınez, G. Photobleaching of sensitisers used in photodynamic therapy. Tetrahedron 2001, 57, 9513–9547. [Google Scholar] [CrossRef]
  51. Kuznetsova, N.A.; Kaliya, O.L. Oxidative photobleaching of phthalocyanines in solution. J. Porphyr. Phthalocyanines 2012, 16, 705–712. [Google Scholar] [CrossRef]
  52. Dilber, G.; Altunparmak, H.; Nas, A.; Kantekin, H.; Durmuş, M. The peripheral and non-peripheral 2H-benzotriazole substituted phthalocyanines: Synthesis, characterization, photophysical and photochemical studies of zinc derivatives. Spectrochim. Acta Part A Mol. Biomol. Spectrosc. 2019, 217, 128–140. [Google Scholar] [CrossRef] [PubMed]
  53. Łapok, Ł.; Cyza, M.; Gut, A.; Kępczyński, M.; Szewczyk, G.; Sarna, T.; Nowakowska, M. Synthesis, spectroscopic properties and interaction with a liposomal membrane of a novel iodinated magnesium phthalocyanine. J. Photochem. Photobiol. A Chem. 2014, 286, 55–63. [Google Scholar] [CrossRef]
  54. Sergeeva, N.N.; Senge, M.O. Photochemical Transformations Involving Magnesium Porphyrins and Phthalocyanines. In PATAI’S Chemistry of Functional Groups; Rappoport, Z., Ed.; John Wiley & Sons, Ltd.: Chichester, UK, 2009; ISBN 978-0-470-68253-1. [Google Scholar]
  55. Piskorz, J.; Mlynarczyk, D.T.; Szczolko, W.; Konopka, K.; Düzgüneş, N.; Mielcarek, J. Liposomal formulations of magnesium sulfanyl tribenzoporphyrazines for the photodynamic therapy of cancer. J. Inorg. Biochem. 2018, 184, 34–41. [Google Scholar] [CrossRef] [PubMed]
  56. Mlynarczyk, D.T.; Dlugaszewska, J.; Falkowski, M.; Popenda, L.; Kryjewski, M.; Szczolko, W.; Jurga, S.; Mielcarek, J.; Goslinski, T. Tribenzoporphyrazines with dendrimeric peripheral substituents and their promising photocytotoxic activity against Staphylococcus aureus. J. Photochem. Photobiol. B Biol. 2020, 204, 111803. [Google Scholar] [CrossRef]
  57. Lakhundi, S.; Zhang, K. Methicillin-Resistant Staphylococcus aureus: Molecular Characterization, Evolution, and Epidemiology. Clin. Microbiol. Rev. 2018, 31, e00020-18. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  58. Masiera, N.; Bojarska, A.; Gawryszewska, I.; Sadowy, E.; Hryniewicz, W.; Waluk, J. Antimicrobial photodynamic therapy by means of porphycene photosensitizers. J. Photochem. Photobiol. B Biol. 2017, 174, 84–89. [Google Scholar] [CrossRef]
  59. Dlugaszewska, J.; Szczolko, W.; Koczorowski, T.; Skupin-Mrugalska, P.; Teubert, A.; Konopka, K.; Kucinska, M.; Murias, M.; Düzgüneş, N.; Mielcarek, J.; et al. Antimicrobial and anticancer photodynamic activity of a phthalocyanine photosensitizer with N -methyl morpholiniumethoxy substituents in non-peripheral positions. J. Inorg. Biochem. 2017, 172, 67–79. [Google Scholar] [CrossRef]
  60. Gajdács, M. The Continuing Threat of Methicillin-Resistant Staphylococcus aureus. Antibiotics 2019, 8, 52. [Google Scholar] [CrossRef] [Green Version]
  61. Walker, M.J.; Barnett, T.C.; McArthur, J.D.; Cole, J.N.; Gillen, C.M.; Henningham, A.; Sriprakash, K.S.; Sanderson-Smith, M.L.; Nizet, V. Disease Manifestations and Pathogenic Mechanisms of Group A Streptococcus. Clin. Microbiol. Rev. 2014, 27, 264–301. [Google Scholar] [CrossRef] [Green Version]
  62. Barnett, T.C.; Bowen, A.C.; Carapetis, J.R. The fall and rise of Group A Streptococcus diseases. Epidemiol. Infect. 2019, 147, e4. [Google Scholar] [CrossRef] [Green Version]
  63. Byrd, A.L.; Belkaid, Y.; Segre, J.A. The human skin microbiome. Nat. Rev. Microbiol. 2018, 16, 143–155. [Google Scholar] [CrossRef] [PubMed]
  64. Dong, Y.; Speer, C.P.; Glaser, K. Beyond sepsis: Staphylococcus epidermidis is an underestimated but significant contributor to neonatal morbidity. Virulence 2018, 9, 621–633. [Google Scholar] [CrossRef] [Green Version]
  65. Mamone, L.; Di Venosa, G.; Gándara, L.; Sáenz, D.; Vallecorsa, P.; Schickinger, S.; Rossetti, M.V.; Batlle, A.; Buzzola, F.; Casas, A. Photodynamic inactivation of Gram-positive bacteria employing natural resources. J. Photochem. Photobiol. B Biol. 2014, 133, 80–89. [Google Scholar] [CrossRef] [PubMed]
  66. Chauke, V.; Durmuş, M.; Nyokong, T. Photochemistry, photophysics and nonlinear optical parameters of phenoxy and tert-butylphenoxy substituted indium(III) phthalocyanines. J. Photochem. Photobiol. A Chem. 2007, 192, 179–187. [Google Scholar] [CrossRef]
  67. Sobotta, L.; Fita, P.; Szczolko, W.; Wrotynski, M.; Wierzchowski, M.; Goslinski, T.; Mielcarek, J. Functional singlet oxygen generators based on porphyrazines with peripheral 2,5-dimethylpyrrol-1-yl and dimethylamino groups. J. Photochem. Photobiol. A Chem. 2013, 269, 9–16. [Google Scholar] [CrossRef]
  68. Seotsanyana-Mokhosi, I.; Kuznetsova, N.; Nyokong, T. Photochemical studies of tetra-2, 3-pyridinoporphyrazines. J. Photochem. Photobiol. A Chem. 2001, 140, 215–222. [Google Scholar] [CrossRef]
  69. Sobotta, L.; Sniechowska, J.; Ziental, D.; Dlugaszewska, J.; Potrzebowski, M.J. Chlorins with (trifluoromethyl)phenyl substituents—Synthesis, lipid formulation and photodynamic activity against bacteria. Dyes Pigments 2019, 160, 292–300. [Google Scholar] [CrossRef]
  70. Sobotta, L.; Dlugaszewska, J.; Kasprzycki, P.; Lijewski, S.; Teubert, A.; Mielcarek, J.; Gdaniec, M.; Goslinski, T.; Fita, P.; Tykarska, E. In vitro photodynamic activity of lipid vesicles with zinc phthalocyanine derivative against Enterococcus faecalis. J. Photochem. Photobiol. B Biol. 2018, 183, 111–118. [Google Scholar] [CrossRef]
  71. Sobotta, L.; Dlugaszewska, J.; Gierszewski, M.; Tillo, A.; Sikorski, M.; Tykarska, E.; Mielcarek, J.; Goslinski, T. Photodynamic inactivation of Enterococcus faecalis by non-peripherally substituted magnesium phthalocyanines entrapped in lipid vesicles. J. Photochem. Photobiol. B Biol. 2018, 188, 100–106. [Google Scholar] [CrossRef]
Figure 1. Chemical structures of studied macrocycles: MgPc, 1, and 2.
Figure 1. Chemical structures of studied macrocycles: MgPc, 1, and 2.
Ijms 21 06145 g001
Figure 2. The UV-vis absorption spectra of studied macrocycles MgPc, 1, 2.
Figure 2. The UV-vis absorption spectra of studied macrocycles MgPc, 1, 2.
Ijms 21 06145 g002
Figure 3. Chemical structures of previously studied macrocycles: sulfanylporphyrazine with isophthaloxybutyl substituents I, porphycene II, chlorin e6 III and phthalocyanine with quaternized morpholinethoxy substituents IV.
Figure 3. Chemical structures of previously studied macrocycles: sulfanylporphyrazine with isophthaloxybutyl substituents I, porphycene II, chlorin e6 III and phthalocyanine with quaternized morpholinethoxy substituents IV.
Ijms 21 06145 g003
Figure 4. The UV-vis absorption and fluorescence spectra (excitation wavelength 325 and 600 nm) in DMF of 1 (a) and 2 (b).
Figure 4. The UV-vis absorption and fluorescence spectra (excitation wavelength 325 and 600 nm) in DMF of 1 (a) and 2 (b).
Ijms 21 06145 g004
Figure 5. The UV-vis absorption spectra in DMF of (a) DPBF decomposition during irradiation of the mixture of 1 and DPBF within 540 s; (b) photobleaching of MgPc within 600 s.
Figure 5. The UV-vis absorption spectra in DMF of (a) DPBF decomposition during irradiation of the mixture of 1 and DPBF within 540 s; (b) photobleaching of MgPc within 600 s.
Ijms 21 06145 g005
Table 1. Fluorescence, photodegradation, singlet oxygen formation quantum yields and log of the molar absorption coefficient of 1, 2 and MgPc.
Table 1. Fluorescence, photodegradation, singlet oxygen formation quantum yields and log of the molar absorption coefficient of 1, 2 and MgPc.
CompoundSolventΦFL106ΦPΦΔlogε[nm]
1DMF0.05186.400.20 [26]4.49[346]4.67[688]
DMSO0.02937.600.274.45[346]4.67[691]
2DMF0.00848.400.05 [26]4.69[346]4.83[690]
DMSO0.0024.840.084.65[346]4.84[693]
MgPcDMF0.230 [46]327 [46]0.28 [46]4.91[346]5.36[670]
DMSO0.1814.770.144.80[346]5.33[673]
ZnPcDMF0.200 [47] 10.2 [48]0.56 [49] -
DMSO0.170 [47] 3.5 [48]0.67 [49]
Table 2. Liposome particle size analysis.
Table 2. Liposome particle size analysis.
CompoundMean Diameter [nm]Dv10 [nm]Dv50 [nm]Dv90 [nm]
1301 ± 3126217629
2188 ± 2371147346
MgPc119 ± 465115171
Table 3. Photodynamic reduction of the bacterial growth by the studied compounds.
Table 3. Photodynamic reduction of the bacterial growth by the studied compounds.
Compound 12MgPc
Concentration [M]log reduction in bacterial growth
Staphylococcus aureus
10−43.5 ± 0.33.6 ± 0.24.9 ± 0.3
10−5>5.9 ± 0.04.3 ± 0.14.6 ± 0.2
MRSA
10−4no activity0.7 ± 0.1>5.7 ± 0.1
10−55.1 ± 0.13.8 ± 0.0>5.7 ± 0.1
Staphylococcus epidermidis
10−43.3 ± 0.23.6 ± 0.2>6.0 ± 0.0
10−5>5.7 ± 0.2>5.7 ± 0.3>5.7 ± 0.4
Streptococcus pyogenes
10−40.5 ± 0.31.7 ± 0.2>4.7 ± 0.3
10−5>4.7 ± 0.3>4.7 ± 0.3>4.7 ± 0.3

Share and Cite

MDPI and ACS Style

Stolarska, M.; Glowacka-Sobotta, A.; Mlynarczyk, D.T.; Dlugaszewska, J.; Goslinski, T.; Mielcarek, J.; Sobotta, L. Photodynamic Activity of Tribenzoporphyrazines with Bulky Periphery against Wound Bacteria. Int. J. Mol. Sci. 2020, 21, 6145. https://doi.org/10.3390/ijms21176145

AMA Style

Stolarska M, Glowacka-Sobotta A, Mlynarczyk DT, Dlugaszewska J, Goslinski T, Mielcarek J, Sobotta L. Photodynamic Activity of Tribenzoporphyrazines with Bulky Periphery against Wound Bacteria. International Journal of Molecular Sciences. 2020; 21(17):6145. https://doi.org/10.3390/ijms21176145

Chicago/Turabian Style

Stolarska, Magdalena, Arleta Glowacka-Sobotta, Dariusz T. Mlynarczyk, Jolanta Dlugaszewska, Tomasz Goslinski, Jadwiga Mielcarek, and Lukasz Sobotta. 2020. "Photodynamic Activity of Tribenzoporphyrazines with Bulky Periphery against Wound Bacteria" International Journal of Molecular Sciences 21, no. 17: 6145. https://doi.org/10.3390/ijms21176145

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop