Elastic Modulus of ECM Hydrogels Derived from Decellularized Tissue Affects Capillary Network Formation in Endothelial Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of Decellularized UBM and SIS
2.3. Evaluation of Decellularization and Histological Structure of Decellularized UBM and SIS
2.4. Composition of Decellularized Tissue
2.4.1. Collagen Content of Decellularized Tissue
2.4.2. Amount of Glycosaminoglycan (GAG) in Decellularized Tissues
2.5. Turbidity Measurement during D-ECM Gelation
2.6. Scanning Electron Microscopy (SEM) of D-ECM Gels
2.7. Compression Test
2.8. Primary Rat Brain Microvascular Endothelial Cell (RBMEC) Culture
2.9. Evaluation of Endothelial Cell Behavior on D-ECM Gels In Vitro
2.10. Capillary Network Formation Analysis of RBMECs
2.11. Statistical Analysis
3. Results
3.1. Cell Contents After Decellularization
3.2. Hydrogel Formation of Decellularized UBM and SIS
3.3. Characterization of D-ECM Hydrogels
3.4. Capillary Network Formation of Endothelial Cells on D-ECM Hydrogels
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ECM | Extracellular matrix |
d-ECM | Decellularized extracellular matrix |
SIS | Small intestine submucosa |
UBM | Urinary bladder matrix |
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Kobayashi, M.; Kadota, J.; Hashimoto, Y.; Fujisato, T.; Nakamura, N.; Kimura, T.; Kishida, A. Elastic Modulus of ECM Hydrogels Derived from Decellularized Tissue Affects Capillary Network Formation in Endothelial Cells. Int. J. Mol. Sci. 2020, 21, 6304. https://doi.org/10.3390/ijms21176304
Kobayashi M, Kadota J, Hashimoto Y, Fujisato T, Nakamura N, Kimura T, Kishida A. Elastic Modulus of ECM Hydrogels Derived from Decellularized Tissue Affects Capillary Network Formation in Endothelial Cells. International Journal of Molecular Sciences. 2020; 21(17):6304. https://doi.org/10.3390/ijms21176304
Chicago/Turabian StyleKobayashi, Mako, Junpei Kadota, Yoshihide Hashimoto, Toshiya Fujisato, Naoko Nakamura, Tsuyoshi Kimura, and Akio Kishida. 2020. "Elastic Modulus of ECM Hydrogels Derived from Decellularized Tissue Affects Capillary Network Formation in Endothelial Cells" International Journal of Molecular Sciences 21, no. 17: 6304. https://doi.org/10.3390/ijms21176304