Adipose-Derived Mesenchymal Stem Cells do not Affect the Invasion and Migration Potential of Oral Squamous Carcinoma Cells
Abstract
:1. Introduction
2. Results
2.1. HSC-3- and MSCs-Conditioned Media had no effect on LINE-1 Methylation
2.2. Microenvironment Remodeling genes Expression of HSC-3 and MSCs cells were Mainly Unaffected when Cultured in MSCs- or HSC-3-Conditioned Media
2.3. AT-MSCs had no Effect on the Invasion and the Proliferation Abilities of HSC-3 or DOK Cells
2.4. AT-MSCs and NOF did not Increase the Migration of HSC-3/DOK Cells
3. Discussion
4. Materials and Methods
4.1. Cell Cultures
4.2. MSCs Characterization
4.3. Culture of MSCs and HSC-3 Cells in Conditioned Media
4.4. Gene Expression Analysis with qRT-PCR
4.5. LINE-1 Methylation Analysis
4.6. Organotypic Myoma Discs
4.7. Immunohistochemical Staining of the Myoma Discs
4.8. Scratch-Wound Cell Migration Assay
4.9. Microscopic and Histomorphometric Analysis
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ANOVA | Analysis of variance |
AT-MSC | Adipose tissue-derived mesenchymal stem cell |
BM-MSC | Bone marrow-derived mesenchymal stem cell |
DOK | Dysplastic oral keratinocyte |
EV | Extracellular vesicle |
HSC-3 | Human tongue squamous carcinoma |
ICAM1 | Intercellular adhesion molecule-1 |
ITGA3 | Integrin alpha-3 |
LINE-1 | Long interspersed nuclear element-1 |
MMP | Matrix metallopeptidase |
MSC | Mesenchymal stem cell |
MS-MLPA | Multiplex ligation-dependent probe amplification |
NOF | Normal oral fibroblast |
OTSCC | Oral tongue squamous cell carcinoma |
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Marker | Clone | % Expression in Cells (Average ± Standard Deviation) | |
---|---|---|---|
AT-MSC (n = 5) | BM-MSC (n = 4) | ||
CD14 | M5E2 | 1.7 ± 2.8 | 0.4 ± 0.3 |
CD19 | HIB19 | 0.3 ± 0.4 | 0.3 ± 0.2 |
CD34 | 581 | 31.9 ± 25.4 | 1.1 ± 1.4 |
CD45RO | UCHL1 | 1.4 ± 2.3 | 0.5 ± 0.3 |
CD54 | HA58 | 61.8 ± 22.8 | 12.4 ± 2.2 |
CD73 | AD2 | 99.0 ± 1.8 | 99.5 ± 0.3 |
CD90 | 5E10 | 99.3 ± 0.7 | 94.6 ± 5.1 |
CD105 | 266 | 96.1 ± 7.1 | 98.2 ± 1.0 |
HLA-DR | G46-6 | 1.7 ± 3.1 | 1.5 ± 0.8 |
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Sinha, S.; Narjus-Sterba, M.; Tuomainen, K.; Kaur, S.; Seppänen-Kaijansinkko, R.; Salo, T.; Mannerström, B.; Al-Samadi, A. Adipose-Derived Mesenchymal Stem Cells do not Affect the Invasion and Migration Potential of Oral Squamous Carcinoma Cells. Int. J. Mol. Sci. 2020, 21, 6455. https://doi.org/10.3390/ijms21186455
Sinha S, Narjus-Sterba M, Tuomainen K, Kaur S, Seppänen-Kaijansinkko R, Salo T, Mannerström B, Al-Samadi A. Adipose-Derived Mesenchymal Stem Cells do not Affect the Invasion and Migration Potential of Oral Squamous Carcinoma Cells. International Journal of Molecular Sciences. 2020; 21(18):6455. https://doi.org/10.3390/ijms21186455
Chicago/Turabian StyleSinha, Snehadri, Matilda Narjus-Sterba, Katja Tuomainen, Sippy Kaur, Riitta Seppänen-Kaijansinkko, Tuula Salo, Bettina Mannerström, and Ahmed Al-Samadi. 2020. "Adipose-Derived Mesenchymal Stem Cells do not Affect the Invasion and Migration Potential of Oral Squamous Carcinoma Cells" International Journal of Molecular Sciences 21, no. 18: 6455. https://doi.org/10.3390/ijms21186455