Drought Disrupts Auxin Localization in Abscission Zone and Modifies Cell Wall Structure Leading to Flower Separation in Yellow Lupine
Abstract
:1. Introduction
2. Results
2.1. Drought Activates AZ Cells and Causes Proline Accumulation
2.2. Soil Water Deficit Affects IAA Localization in Floral AZ
2.3. Drought Induces Changes in the Composition of Cell Wall Components
3. Discussion
4. Material and Methods
4.1. Plant Material, Growth Conditions
4.2. Material Fixation
4.3. Histochemical Analysis of AZ Structure
4.4. Immunocytochemical Assay
4.5. IAA GC-MS Analysis
4.6. Extraction and Determination of Pectin and Hemicellulose
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ABA | abscisic acid |
AZ | abscission zone |
DIST | flower pedicel fragments above AZ |
ET | ethylene |
H2O2 | hydrogen peroxide |
HG | homogalacturonans |
IAA | Indole-3-acetic acid |
PG | polygalacturonase |
PIN | PIN-FORMED |
PME | pectin methylesterase |
PROX | stem fragments below the AZ |
RG | rhamnogalacturonans |
ROS | reactive oxygen species |
TIBA | 2,3,5-triiodobenzoic acid |
TIR1 | TRANSPORT INHIBITOR RESPONSE 1 |
VB | vascular bundles |
WHC | water holding capacity |
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Florkiewicz, A.B.; Kućko, A.; Kapusta, M.; Burchardt, S.; Przywieczerski, T.; Czeszewska-Rosiak, G.; Wilmowicz, E. Drought Disrupts Auxin Localization in Abscission Zone and Modifies Cell Wall Structure Leading to Flower Separation in Yellow Lupine. Int. J. Mol. Sci. 2020, 21, 6848. https://doi.org/10.3390/ijms21186848
Florkiewicz AB, Kućko A, Kapusta M, Burchardt S, Przywieczerski T, Czeszewska-Rosiak G, Wilmowicz E. Drought Disrupts Auxin Localization in Abscission Zone and Modifies Cell Wall Structure Leading to Flower Separation in Yellow Lupine. International Journal of Molecular Sciences. 2020; 21(18):6848. https://doi.org/10.3390/ijms21186848
Chicago/Turabian StyleFlorkiewicz, Aleksandra Bogumiła, Agata Kućko, Małgorzata Kapusta, Sebastian Burchardt, Tomasz Przywieczerski, Grażyna Czeszewska-Rosiak, and Emilia Wilmowicz. 2020. "Drought Disrupts Auxin Localization in Abscission Zone and Modifies Cell Wall Structure Leading to Flower Separation in Yellow Lupine" International Journal of Molecular Sciences 21, no. 18: 6848. https://doi.org/10.3390/ijms21186848