Phospholipase D1 and D2 Synergistically Regulate Thrombus Formation
Abstract
:1. Introduction
2. Results
2.1. Effects of the PLD1 and PLD2 Inhibitors in Adenosine 5′-Diphosphate (ADP)-Induced Acute Pulmonary Thrombosis in Mice
2.2. Effects of PLD1 and PLD2 Inhibitors on Transient MCAO-Induced Brain Injury
2.3. Effects of PLD1 and PLD2 Inhibitors on Neurobehavioral Function after Transient MCAO
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Animals
4.3. Acute Pulmonary Thrombosis Induced by ADP in Mice
4.4. Transient MCAO-Induced Brain Injury in Mice
4.5. Modified Neurological Severity Scores
4.6. Rotarod Test
4.7. Open-Field Test
4.8. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
PLD | Phospholipase D |
FeCl3 | Ferric chloride |
FIPI | 5-fluoro-2-indolyl des-chlorohalopemide |
MCA | Middle cerebral artery |
MCAO | Middle cerebral artery occlusion |
ADP | Adenosine 5′-diphosphate |
TTC | 2,3,5-triphenyltetrazolium chloride |
DMSO | Dimethyl sulphoxide |
HE | Hematoxylin-eosin |
mNSSs | Modified neurological severity scores |
rpm | Revolutions per min |
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Share and Cite
Lien, L.-M.; Lu, W.-J.; Chen, T.-Y.; Lee, T.-Y.; Wang, H.-H.; Peng, H.-Y.; Chen, R.-J.; Lin, K.-H. Phospholipase D1 and D2 Synergistically Regulate Thrombus Formation. Int. J. Mol. Sci. 2020, 21, 6954. https://doi.org/10.3390/ijms21186954
Lien L-M, Lu W-J, Chen T-Y, Lee T-Y, Wang H-H, Peng H-Y, Chen R-J, Lin K-H. Phospholipase D1 and D2 Synergistically Regulate Thrombus Formation. International Journal of Molecular Sciences. 2020; 21(18):6954. https://doi.org/10.3390/ijms21186954
Chicago/Turabian StyleLien, Li-Ming, Wan-Jung Lu, Ting-Yu Chen, Tzu-Yin Lee, Hsueh-Hsiao Wang, Hsien-Yu Peng, Ray-Jade Chen, and Kuan-Hung Lin. 2020. "Phospholipase D1 and D2 Synergistically Regulate Thrombus Formation" International Journal of Molecular Sciences 21, no. 18: 6954. https://doi.org/10.3390/ijms21186954