The Role of Saccharides in the Mechanisms of Pathogenicity of Fusarium oxysporum f. sp. lupini in Yellow Lupine (Lupinus luteus L.)
Abstract
:1. Introduction
2. Results
2.1. Effects of Saccharides on the Growth and Sporulation of F. oxysporum f. sp. lupini
2.2. Effects of Saccharides on the Accumulation of Moniliformin in Embryo Axes of L. luteus Infected with F. oxysporum f. sp. lupini
2.3. Effects of Saccharides on the Accumulation of Ergosterol in Embryo Axes of L. luteus Infected with F. oxysporum f. sp. lupini
2.4. Effects of Saccharides and F. oxysporum f. sp. lupini Infection on the Growth of Yellow Lupine Embryo Axes
2.5. Correlation Analysis
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Preparation of Spore Suspension and Inoculation
4.3. Analysis of the Impact of Soluble Carbohydrates on Mycelium Growth and Sporulation
4.4. Determination of Ergosterol (ERG)
4.5. Determination of Moniliformin (MON)
4.6. Analysis of the Impact of Soluble Carbohydrates and F. oxysporum f. sp. lupini Infection on the Growth of Yellow Lupine Embryo Axes
4.7. Statistics
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
+Sn | non-inoculated embryo axes and cultured in vitro on Heller’s medium with 60 mM sucrose |
+Gn | non-inoculated embryo axes and cultured in vitro on medium with 120 mM glucose |
+Fn | non-inoculated embryo axes and cultured in vitro on medium with 120 mM fructose |
−Sn | non-inoculated cultured in vitro on medium without sucrose |
+Si | inoculated and cultured with 60 mM sucrose |
+Gi | inoculated and cultured with 120 mM glucose |
+Fi | inoculated and cultured with 120 mM fructose |
−Si | inoculated and cultured without sucrose |
ERG | ergosterol |
MON | moniliformin |
WA | water agar |
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Formela-Luboińska, M.; Remlein-Starosta, D.; Waśkiewicz, A.; Karolewski, Z.; Bocianowski, J.; Stępień, Ł.; Labudda, M.; Jeandet, P.; Morkunas, I. The Role of Saccharides in the Mechanisms of Pathogenicity of Fusarium oxysporum f. sp. lupini in Yellow Lupine (Lupinus luteus L.). Int. J. Mol. Sci. 2020, 21, 7258. https://doi.org/10.3390/ijms21197258
Formela-Luboińska M, Remlein-Starosta D, Waśkiewicz A, Karolewski Z, Bocianowski J, Stępień Ł, Labudda M, Jeandet P, Morkunas I. The Role of Saccharides in the Mechanisms of Pathogenicity of Fusarium oxysporum f. sp. lupini in Yellow Lupine (Lupinus luteus L.). International Journal of Molecular Sciences. 2020; 21(19):7258. https://doi.org/10.3390/ijms21197258
Chicago/Turabian StyleFormela-Luboińska, Magda, Dorota Remlein-Starosta, Agnieszka Waśkiewicz, Zbigniew Karolewski, Jan Bocianowski, Łukasz Stępień, Mateusz Labudda, Philippe Jeandet, and Iwona Morkunas. 2020. "The Role of Saccharides in the Mechanisms of Pathogenicity of Fusarium oxysporum f. sp. lupini in Yellow Lupine (Lupinus luteus L.)" International Journal of Molecular Sciences 21, no. 19: 7258. https://doi.org/10.3390/ijms21197258