eIF2α Phosphorylation by GCN2 Is Induced in the Presence of Chitin and Plays an Important Role in Plant Defense against B. cinerea Infection
Abstract
:1. Introduction
2. Results
2.1. gcn2 Mutants Show Impaired Defense Response against Botrytis cinerea
2.2. eIF2α Is Phosphorylated upon Plant Contact with B. cinerea Spores and This Phosphorylation Is Dependent on GCN2
2.3. Chitin Induces GCN2-Dependent eIF2α Phosphorylation
2.4. Chitin Promotes a Global Reduction of Translation
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Phytopathogens, Storage, and Growth Conditions
4.3. Pathogen Inoculation and Phenotypic Assessment
4.4. Gene Expression Analysis by Quantitative Real-Time PCR (qRT-PCR)
4.5. eIF2α Phosphorylation Analysis
4.6. Metabolic Labeling of Newly Synthesized Proteins
4.7. Polysome Profiling
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
GCN2 | general control non-derepressible 2 |
eIF2α | eukaryotic initiation factor 2 subunit α |
SA | salicylic acid |
JA | jasmonic acid |
MeJA | methyl jasmonate |
ET | ethylene |
ACC | 1-aminocyclopropane-1-carboxylic acid |
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Berrocal-Lobo, M.; Toribio, R.; Castellano, M.M. eIF2α Phosphorylation by GCN2 Is Induced in the Presence of Chitin and Plays an Important Role in Plant Defense against B. cinerea Infection. Int. J. Mol. Sci. 2020, 21, 7335. https://doi.org/10.3390/ijms21197335
Berrocal-Lobo M, Toribio R, Castellano MM. eIF2α Phosphorylation by GCN2 Is Induced in the Presence of Chitin and Plays an Important Role in Plant Defense against B. cinerea Infection. International Journal of Molecular Sciences. 2020; 21(19):7335. https://doi.org/10.3390/ijms21197335
Chicago/Turabian StyleBerrocal-Lobo, Marta, René Toribio, and M. Mar Castellano. 2020. "eIF2α Phosphorylation by GCN2 Is Induced in the Presence of Chitin and Plays an Important Role in Plant Defense against B. cinerea Infection" International Journal of Molecular Sciences 21, no. 19: 7335. https://doi.org/10.3390/ijms21197335