Modulation of (Homo)Glutathione Metabolism and H2O2 Accumulation during Soybean Cyst Nematode Infections in Susceptible and Resistant Soybean Cultivars
Abstract
:1. Introduction
2. Results
2.1. Root Penetration
2.2. (h) GSH Metabolism Was Modified in Nematode-Induced Soybean Roots
2.3. Quantitative Detection of ROS Production
2.4. (h)GSH Deficiency Impairs Nematode Development and Reproduction
2.5. Association between (h)GSH Deficiency and H2O2 Accumulation in Response to SCN
3. Discussion
4. Materials and Methods
4.1. Plant Material, Growth Condition, and Treatments
4.2. Nematode Inoculum
4.3. Root Penetration Studies
4.4. Total RNA Extraction and Gene Expression Analysis
4.5. γ-Glu-Cys and (h)GSH Determination
4.6. H2O2 Quantification and Cytochemical Detection
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
γ-Glu-Cys | γ-Glutamylcysteine |
GSH | Reduced glutathione |
GSSG | Oxidized glutathione |
hGSH | homoGlutathione |
γ-ECS | γ-Glutamylcysteine synthetase |
GSHS | Glutathione synthetase |
hGSHS | homoGlutathione synthetase |
ROS | Reactive oxygen species |
BSO | L-buthionine sulfoximine |
H2O2 | Hydrogen peroxide |
O2− | Superoxide |
HR | Hypersensitive reaction |
RKN | Root-knot nematode |
SCN | Soybean cyst nematode |
J2 | Second-stage juvenile |
J3 | Third-stage juvenile |
J4 | Fourth-stage juvenile |
dpi | Days post-inoculation |
NaOCl | Sodium hypochlorite |
SDW | Sterile distilled water |
HPLC | High-performance liquid chromatography |
qRT-PCR | Quantitative reverse transcription-PCR |
CLSM | Confocal laser-scanning microscope |
SA | Salicylic acid |
JA | Jasmonic acid |
ET | Ethylene |
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Chen, X.; Li, S.; Zhao, X.; Zhu, X.; Wang, Y.; Xuan, Y.; Liu, X.; Fan, H.; Chen, L.; Duan, Y. Modulation of (Homo)Glutathione Metabolism and H2O2 Accumulation during Soybean Cyst Nematode Infections in Susceptible and Resistant Soybean Cultivars. Int. J. Mol. Sci. 2020, 21, 388. https://doi.org/10.3390/ijms21020388
Chen X, Li S, Zhao X, Zhu X, Wang Y, Xuan Y, Liu X, Fan H, Chen L, Duan Y. Modulation of (Homo)Glutathione Metabolism and H2O2 Accumulation during Soybean Cyst Nematode Infections in Susceptible and Resistant Soybean Cultivars. International Journal of Molecular Sciences. 2020; 21(2):388. https://doi.org/10.3390/ijms21020388
Chicago/Turabian StyleChen, Xi, Shuang Li, Xuebing Zhao, Xiaofeng Zhu, Yuanyuan Wang, Yuanhu Xuan, Xiaoyu Liu, Haiyan Fan, Lijie Chen, and Yuxi Duan. 2020. "Modulation of (Homo)Glutathione Metabolism and H2O2 Accumulation during Soybean Cyst Nematode Infections in Susceptible and Resistant Soybean Cultivars" International Journal of Molecular Sciences 21, no. 2: 388. https://doi.org/10.3390/ijms21020388