Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice
Abstract
:1. Introduction
2. Results
2.1. Production and Analysis of OsEXPA7-OX Rice Plants
2.2. OsEXPA7 Overexpressing Lines Showed Strong Tolerance to Saline Stress
2.3. OsEXPA7 Was Mainly Expressed in Shoot Apical Meristem (SAM), Root, and Leaf Sheath
2.4. Morphological Changes in Leaves and Roots Due to OsEXPA7 Overexpression in Rice
2.5. Improved Ion Homeostasis in OsEXPA7-OX Plants Under Salt Stress
2.6. Overexpression of OsEXPA7 Improved the Antioxidant Capacity of Transgenic Rice Lines
2.7. DEGs in OsEXPA7-OX and WT Plants
2.8. Functional Classification of DEGs Using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway Analysis
2.9. Genes Involved in Salt Stress Tolerance were Significantly Altered in OsEXPA7-OX Compared to WT
3. Discussion
3.1. OsEXPA7 Overexpression Improved Salt Stress Tolerance in Rice
3.2. OsEXPA7 Overexpression Increased Cell Size and Enhanced Plant Growth
3.3. Improvement of Na+ and K+ Balance in OsEXPA7-OX Plants Under Salt Stress
3.4. Overexpression of OsEXPA7 Improved Oxidative Stress Tolerance
3.5. Possible Mechanisms Underlying Salt Tolerance in OsEXPA7 Overexpressing Rice Plants
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Morphological Analysis
4.3. Measurement of Electrical Conductivity and Chlorophyll Concentration
4.4. Histochemical Assay
4.5. Determination of Na+ and K+ Content
4.6. Determination of ROS and Enzyme Activity
4.7. MDA and Proline Content
4.8. Real-Time PCR Analysis and cDNA Synthesis
4.9. RNA Isolation and RNA-Seq Analysis
4.10. Data Analyses
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
CaMV | Cauliflower mosaic virus |
PGD | Phosphogluconate dehydrogenase |
GUS | b-glucuronidase |
WT | Wild type |
ROS | Reactive oxygen species |
MDA | Malondialdehyde |
OX | Overexpression |
GO | Gene ontology |
LV | Large vein |
BS | Bundle sheath |
Co | Collenchyma |
MX | Metaxylem |
ARF | Auxin response factor |
SOS1 | Salt overly sensitive 1 |
NHX4 | Sodium/hydrogen exchanger 4 |
PM | Plasma membrane |
NaCl | Sodium chloride |
DAB | 3,3-diaminobenzidine |
NBT | Nitro-blue tetrazolium |
DEG | Differentially expressed genes |
FDR | False discovery rate |
qRT-PCR | Quantitative real-time polymerase chain reaction |
Aux/IAA | Auxin/indole-3-acetic acid |
SAM | Shoot apical meristem |
POD | Peroxidase |
SOD | Superoxide dismutase |
KEGG | Kyoto Encyclopedia of Genes and Genomes |
WAK | Wall-associated kinase |
ELR | Electrolyte leakage ratio |
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Gene Name | Gene ID | Up or Down Ratio (Log2-Fold Change) | Function |
---|---|---|---|
OsSOS1 | LOC_Os12g44360.1 | up (2.35) | Sodium/hydrogen exchanger 7, putative, expressed |
OsARF23 | LOC_Os11g32110.3 | up (25.30) | auxin response factor, putative, expressed |
OsWAK1 | LOC_Os11g46860.1 | up (3.93) | wall-associated receptor kinase 3 precursor |
OsMSRA4.1 | LOC_Os10g41400.2 | up (4.64) | response to salt stress, cellular response to oxidative stress |
OsWRKY71 | LOC_Os02g08440.2 | up (6.00) | Response to salt stress, response to ethylene, response to abscisic acid |
OsSAPK2 | LOC_Os07g42940.6 | up (3.30) | serine/threonine-protein kinase SAPK2 |
OsGH3.12 | LOC_Os11g08340.1 | up (2.15) | Auxin-responsive Glycoside Hydrolase 3 (GH3) family member |
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Jadamba, C.; Kang, K.; Paek, N.-C.; Lee, S.I.; Yoo, S.-C. Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice. Int. J. Mol. Sci. 2020, 21, 454. https://doi.org/10.3390/ijms21020454
Jadamba C, Kang K, Paek N-C, Lee SI, Yoo S-C. Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice. International Journal of Molecular Sciences. 2020; 21(2):454. https://doi.org/10.3390/ijms21020454
Chicago/Turabian StyleJadamba, Chuluuntsetseg, Kiyoon Kang, Nam-Chon Paek, Soo In Lee, and Soo-Cheul Yoo. 2020. "Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice" International Journal of Molecular Sciences 21, no. 2: 454. https://doi.org/10.3390/ijms21020454
APA StyleJadamba, C., Kang, K., Paek, N. -C., Lee, S. I., & Yoo, S. -C. (2020). Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice. International Journal of Molecular Sciences, 21(2), 454. https://doi.org/10.3390/ijms21020454