Next Article in Journal
Resveratrol Rescue Indoxyl Sulfate-Induced Deterioration of Osteoblastogenesis via the Aryl Hydrocarbon Receptor /MAPK Pathway
Previous Article in Journal
Positron Emission Tomography (PET) and Neuroimaging in the Personalized Approach to Neurodegenerative Causes of Dementia
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 21
Issue 20
10.3390/ijms21207482
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Review

Jasmonic Acid at the Crossroads of Plant Immunity and Pseudomonas syringae Virulence

by
Aarti Gupta
1,
Mamta Bhardwaj
2 and
Lam-Son Phan Tran
3,4,*
1
Department of Life Sciences, POSTECH Biotech Center, Pohang University of Science and Technology, Pohang 37673, Korea
2
Department of Botany, Hindu Girls College, Maharshi Dayanand University, Sonipat 131001, India
3
Institute of Research and Development, Duy Tan University, 03 Quang Trung, Da Nang 550000, Vietnam
4
Stress Adaptation Research Unit, RIKEN Center for Sustainable Resource Science, 1-7-19 22, Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(20), 7482; https://doi.org/10.3390/ijms21207482
Submission received: 20 July 2020 / Revised: 6 October 2020 / Accepted: 7 October 2020 / Published: 11 October 2020
(This article belongs to the Section Molecular Plant Sciences)
Browse Figures
Versions Notes

Abstract

:
Sensing of pathogen infection by plants elicits early signals that are transduced to affect defense mechanisms, such as effective blockage of pathogen entry by regulation of stomatal closure, cuticle, or callose deposition, change in water potential, and resource acquisition among many others. Pathogens, on the other hand, interfere with plant physiology and protein functioning to counteract plant defense responses. In plants, hormonal homeostasis and signaling are tightly regulated; thus, the phytohormones are qualified as a major group of signaling molecules controlling the most widely tinkered regulatory networks of defense and counter-defense strategies. Notably, the phytohormone jasmonic acid mediates plant defense responses to a wide array of pathogens. In this review, we present the synopsis on the jasmonic acid metabolism and signaling, and the regulatory roles of this hormone in plant defense against the hemibiotrophic bacterial pathogen Pseudomonas syringae. We also elaborate on how this pathogen releases virulence factors and effectors to gain control over plant jasmonic acid signaling to effectively cause disease. The findings discussed in this review may lead to ideas for the development of crop cultivars with enhanced disease resistance by genetic manipulation.

1. Introduction

An incidence of virulent pathogen infection in a plant, which is incapable of surmounting effective immune responses, leads to the appearance of plant disease. A resistant plant, on the other hand, is well equipped with a versatile arsenal and employs different signaling molecules to recognize and thwart away pathogens. Jasmonic acid (JA) and its derived molecules (mainly, methyl jasmonate, MeJA, and isoleucine-conjugated jasmonate, JA-Ile) are commonly designated as jasmonates (JAs), a subgroup of oxylipins derived from 12-oxo-phytodienoic acid (OPDA) [1]. JAs have been associated with plant defense against herbivory and necrotrophic pathogen attacks [2]. On the other hand, JAs suppress plant resistance responses against biotrophic and hemibiotrophic pathogens, while salicylic acid (SA) is known to promote effective disease resistance responses to these pathogens [3,4]. This antagonism between SA and JAs has been further supported by studies in Arabidopsis thaliana where the SA-mediated signaling is noted to suppress the JA signaling, thereby protecting plants against biotrophic or hemibiotrophic pathogen infections [2,3,5,6]. This antagonism is very well exploited by biotrophic and hemibiotrophic pathogens to promote JA synthesis and signaling, thereby weakening the SA-dependent plant defense to infect the host plants [6,7,8,9].
However, a few emerging reports have associated JAs with positive regulation of plant immunity during hemibiotrophic pathogen infection [10]. Besides, hemibiotrophic pathogen infection increases endogenous JA concentrations in Arabidopsis [11]. From the published reports, it is now apparent that JA action can be dependent or independent of any SA functioning, while modulating plant immunity to hemibiotrophic pathogen infections. Thus, the regulatory roles of JAs in plant resistance responses under biotrophic and hemibiotrophic pathogen infections appear to be very enigmatic. In this review, the biological functions of JAs and associated signaling are discussed with respect to the plant immunity against the infections by hemibiotrophic pathogens, and how these pathogens can modulate JA signaling. Pseudomonas syringae is a major hemibiotrophic bacterial pathogen, which wreaks havoc in a multitude of host plants, such as Arabidopsis, tomato (Solanum lycopersicum), and tobacco (Nicotiana tabacum). This review encompasses the regulatory roles of JAs in plant immunity, by mostly reviewing the latest literature on the infections of P. syringae in various plant species.

2. JA Metabolism

The JA biosynthesis pathway in different plant species was first construed by Vick and Zimmerman [12], which is catalyzed in three different locations, starting from chloroplasts to peroxisomes and culminating in the cytoplasm (Figure 1a). Briefly, JA biosynthesis initiates with the 13-LIPOXYGENASE (LOX)-mediated oxygenation of α-linolenic acid (18:3) in the chloroplast membrane. Subsequent reactions catalyzed by ALLENE OXIDE SYNTHASE (AOS, a CYTOCHROME P450 CYP74) and ALLENE OXIDE CYCLASE (AOC) generate cyclopentenone OPDA (18 carbons) and dinor-oxo-phytodienoic acid (dnOPDA, a 16-carbon homolog of OPDA) (Figure 1a). The OPDA is then exported from chloroplasts by JASSY (a membrane channel that is located in the outer envelope of chloroplasts) [13] and imported into peroxisomes by COMATOSE (CTS), an ABC transporter located in the peroxisome membrane [14]. Subsequently, OPDA REDUCTASE 3 (OPR3) converts OPDA to 3-oxo-2(2′[Z]-pentenyl)-cyclopentane-1-octanoic acid (OPC8), which in turn, undergoes three consecutive reactions of β-oxidation catalyzed by ACYL-CoA-OXIDASE 1 (ACX1) to produce JA (Figure 1a). The nascent JA produced in peroxisomes is eventually translocated to the cytoplasm for various metabolic alterations, including hydroxylation, decarboxylation, glycosylation, methylation, and amino acid conjugation [15,16]. JA is methylated by S-ADENOSYL-L-METHIONINE:JASMONIC ACID CARBOXYL METHYLTRANSFERASE (JMT) to form MeJA [15] (Figure 1a). Besides, JA also undergoes amino acid conjugation by the action of JASMONATE RESISTANT 1 (JAR1), a jasmonate-amido synthetase, to yield JA-Ile [(+)-7-iso-JA–L-Ile] [16] (Figure 1a). MeJA and JA-Ile are the most common biologically active JA forms reported in plants [17]. In Arabidopsis opr3, mutants that lack a functional OPR3-dependent JA biosynthetic pathway, the OPDA is demonstrated to adopt an alternate route and undergoes β-oxidation reactions to generate 4,5-didehydro-jasmonic acid (4,5-ddh-JA, an OPDA derivative), which is released into the cytoplasm where it is reduced by OPR2 in an OPR3-independent pathway to synthesize JA [18,19] (Figure 1a).
The JA catabolism has been well-demonstrated in higher plants for its role in maintaining the JA homeostasis during a switch from inducible to non-inducible conditions. Three distinct JA-catabolic pathways are well demonstrated in Arabidopsis (Figure 1b). In the first pathway, JA catabolism is majorly attributed to the hydroxylation of JA-Ile by the CYTOCHROME P450 MONOOXYGENASE CYP94 family members in an ω-oxidation reaction [20,21,22,23,24,25]. The CYP94B3 (a jasmonoyl-isoleucine-12-hydroxylase) and its less predominant homolog CYP94B1 catalyze mono-oxygenation of JA-Ile to a weak analog, 12-hydroxy-jasmonic acid isoleucine (12OH-JA-Ile), whereas CYP94C1 along with CYP94B3 catalyze the double oxygenation of JA-Ile to yield an inactive form, 12-carboxy-jasmonic acid isoleucine (12COOH-JA-Ile) [21,22,23,24,25]. Both CYP94B3 and CYP94C1 reside on endoplasmic reticulum (ER), and hence, associate JA inactivation with the ER site [22] (Figure 1b, (i)). The second pathway involves the deconjugation of the JA–amino acid conjugates by amidohydrolases encoded by INDOLE ACETIC ACID (IAA)-ALANINE RESISTANT 3 (IAR3)- and IAA-LEUCINE RESISTANT (ILR)-LIKE GENE 6 (ILL6), which cleave JA-Ile to free JA and disrupt JA-Ile-dependent signaling [20,21,22,23,24,25,26] (Figure 1b, (ii)). The third alternative route for maintaining JA homeostasis is catalyzed by JASMONATE-INDUCED OXYGENASE (JOX)-mediated direct hydroxylation of JA to the inactive 12-hydroxy-jasmonic acid (12OH-JA) [27] (Figure 1b, (iii)). However, the subcellular location of the enzyme and its action is not known yet. Besides, the amidohydrolases IAR3 and ILL6 also breakdown 12OH-JA-Ile conjugates downstream of CYP94Bs in the first pathway to yield the inactive 12OH-JA [28] (Figure 1b, (i)). Evidence for subcellular location of IAR3 and ILL6 is still lacking; however, based on the fact that these two proteins act downstream of the ER-localized CYP94s and the prediction results obtained using the “Subcellular Localisation Database for Arabidopsis Proteins” database (https://suba.live), we tentatively placed this branch in the ER.

3. JA Signaling

CORONATINE INSENSITIVE 1 (COI1) receptor and JASMONATE ZINC-FINGER EXPRESSED IN INFLORESCENCE MERISTEM (ZIM)-DOMAIN (JAZ) proteins are the integral components of the JA signaling [29,30]. COI1 is a nuclear F-box component of an S PHASE KINASE-ASSOCIATED PROTEIN 1 (SKP1)-CULLIN-F-BOX (SCF)-type E3 ubiquitin ligase complex, and JAZs are negative regulators of JA-induced genes and are tagged by the SCFCOI1 complex for the 26S proteasome-mediated degradation [29,30].
In the absence or scarcity of endogenous JAs, JAZ proteins form a complex with the co-repressor TOPLESS (TPL), TPL-RELATED (TPR) proteins and HISTONE DEACETYLASE (HDA) (Figure 2a). This consolidated repressor complex then binds to and represses the actions of positive regulators of the JA signaling, such as the MYELOCYTOMATOSIS (MYC)-type transcription factors (TFs) that consist of both basic helix-loop-helix (bHLH) and leucine zipper motifs [31]. The association of JAZs with TPL and TPR proteins is mediated by an adaptor protein called NOVEL INTERACTOR OF JAZ (NINJA) [32]. This JAZNINJA-TPL-TPR repressor complex tightly holds the bound TFs and inhibits them from activating the transcription of downstream JA-responsive genes [1,31,32,33] (Figure 2a).
However, upon elicitation, once the JA-Ile accumulates in the cytoplasm, it is imported into the nucleus by JASMONATE TRANSPORTER 1 (JAT1, an ABC transporter) located in the nuclear envelope [36]. The imported JA-Ile, in turn, binds to the COI1 receptor, causing a conformational change in the COI1 receptor (Figure 2b). The COI1–JA-Ile complex then enrolls JAZ proteins where the COI1 instigates the 26S proteasome-mediated degradation of the JAZ repressors [7,30] (Figure 2b). The inositol pentakisphosphate (InsP5) molecule functions as a co-receptor for JA-Ile and stabilizes the association of COI1–JAZ complex [9,37] (Figure 2b). As a result of JA-triggered degradation of JAZs and disintegration of the repressor complex, the repression on bound TFs is released, allowing them to induce the transcription of downstream JA-responsive genes [9,29,30,32,33]. Depending upon the nature of the elicitor, the JAZ proteins can bind to different classes of TFs. As a result, JA signaling bifurcates into the MYC and the ETHYLENE RESPONSE FACTOR (ERF) branches [40,41,42,43] (Figure 2b). These two branches are primarily governed by the MYC2 (Figure 2b, (i)), and the ETHYLENE-INSENSITIVE 3 (EIN3) and ETHYLENE-INSENSITIVE 3-LIKE 1 (EIL1) (Figure 2b, (ii)) TFs, respectively, and are mutually antagonistic [39]. MYC2 interacts with EIN3 and EIL1 proteins and inhibits their transactivation activities in inducing the downstream target genes, which thereby represses plant responses primarily governed by the ethylene (ET) [43]. Conversely, EIN3 and EIL1 also interact with and inhibit the transcriptional activity of MYC2, thereby inhibiting MYC2-target gene expression and attenuating JA-governed plant responses [43].

3.1. The MYC Branch

The MYC branch specifically regulates the wounding- and insect-induced JA responses and is governed by the MYC2 TF. In addition, other members of the MYC family, specifically MYC3 and MYC4, act in conjunction with MYC2 to activate the transcription of JA-responsive marker genes such as VEGETATIVE STORAGE PROTEIN 2 (VSP2) [40,44] (Figure 2b, (i)). In the presence of stress-induced JA-Ile levels, the COI1-dependent degradation of the JAZ repressor complex relieves its repression activity on MYC TFs (i.e., MYC2, 3, and 4). In turn, the liberated MYC2 binds the interacting domain of the MED25 subunit of the MEDIATOR transcriptional co-activator complex [38,45,46]. This MYC2–MED25 complex then recruits the HISTONE ACETYLTRANSFERASE OF THE CBP FAMILY 1 (HAC1), along with an RNA POLYMERASE II, and other required general TFs (GTFs) to the MYC-bound promoters to initiate JA-responsive transcriptional reprogramming [38,45,46]. MED25 physically and functionally interacts with HAC1, which acetylates lysine 9 (K9) in histone H3, specifically at the MYC2-target promoters, thereby favoring gene activation [46] (Figure 2b, (i)). In contrast, under non-induced conditions, when the endogenous JA-Ile levels are below a threshold, the Jas motif of the JAZ proteins conforms to an extended α-helix structure that binds to the N-terminal JAZ-interacting domain (JID) of MYCs [47] (Figure 2a, (i)). The conformational change in the α-helix of the JAZ domain prevents the binding of MYC2 to the MED25 [47], thereby repressing the JA output. The MED25 also interacts physically with COI1, and thus, the MED25–COI1 complex remains stable under the non-inducible conditions [46].

3.2. The ERF Branch

The ERF branch is activated specifically upon necrotrophic pathogen attack and is characterized by the transcriptional activation of the gene encoding a PLANT DEFENSIN 1.2 (PDF1.2) [39,48]. This branch is coordinated by the synergism between ET- and JA-signaling pathways where JA accumulation and perception by COI lead to the breakdown of JAZ proteins and subsequent release of JAZ-bound positive regulators EIN3 and EIL1, and ET-induced signaling stabilizes these two TFs [34]. Under non-induced JA levels, JAZs directly interact with EIN3 and EIL1 and repress their functioning, or JAZ proteins recruit the transcriptional co-repressor HDA6 [34,35] (Figure 2a, (ii)). The HDA6-mediated deacetylation of histones (especially H4) obstructs EIN3 and EIL1 to bind to the target gene promoters, thereby inhibiting the subsequent expression of downstream genes (e.g., ERF1, OCTADECANOID-RESPONSIVE ARABIDOPSIS AP2 (APETALA2)/ERF-DOMAIN PROTEIN 59 (ORA59) and PDF1.2) and suppressing JA signaling [34] (Figure 2a, (ii)). Direct interaction of HDA6 with EIN3 and EIL1 has also been demonstrated [34]. In addition to the HDA6, the HDA19-mediated histone modification is also regarded as a key factor in the regulation of JA signaling [49]. In the presence of necrotroph-induced accumulation of JAs, the JAZ proteins are degraded, and the EIN3 and EIL1 TFs are released from the repression by JAZ and HDA6 proteins, allowing them to activate the transcription of ORA59 and ERF1 [34,39]. The ORA59 and ERF1 TFs, in turn, recruit MED25 and bind to the GCC-box motif present in the promoter regions of downstream genes via the ERF domain and activate their transcription [50] (Figure 2b, (ii)).

3.3. Negative Regulation of JA Signaling

JA signaling is tightly regulated in plant cells and is negatively regulated at multiple stages in the absence of the inducer or during the recovery phase (Figure 3). In addition to binding to MYC2, EIN3, and EIL1 TFs, JAZs have also been shown to bind to other transcriptional regulators belonging to the bHLH class of TFs, such as JA-ASSOCIATED MYC2-LIKE 1 (JAM1) and JAM2, which in turn, bind to the MYC2-target promoters as transcriptional repressors to antagonize MYC2 action and negatively influence JA-dependent responses [51,52] (Figure 3a). It was also reported that JAZ8 interacts with JASMONATE-ASSOCIATED VQ MOTIF GENE 1 (JAV1), which is a suppressor of JA-dependent defense responses, and WRKY51 TF to form a “JJW” co-repressor complex [53]. The JJW complex, in turn, binds to the promoter of the JA-biosynthetic genes (e.g., AOS gene) and represses their expression, inhibiting JA biosynthesis [53] (Figure 3b). In addition to the JAZ- or JAM-mediated repression of the MYC2 functioning, the activities of MYC2, MYC3, and MYC4 are also regulated at protein levels. The MYC2 protein is polyubiquitinated by PLANT U-BOX PROTEIN 10 (PUB10), an E3 ligase, and is targeted for degradation, leading to suppression of the JA-responsive PDF1.2 gene [54]. However, in vitro experiments have shown that the polyubiquitinated MYC2 can be deubiquitinated by UBIQUITIN-SPECIFIC PROTEASE proteins UBP12 and UBP13, which extends the half-life of MYC2 and activates the JA responses [55] (Figure 3c). In a recent study, a negative-feedback regulatory mechanism controlling the MYC2 levels and activity has been suggested [56]. The BROAD COMPLEX, TRAMTRACK, BRIC-A-BRAC/POX VIRUS, AND ZINC FINGER (BTB/POZ) domain and MEPRIN AND TRAF HOMOLOGY (MATH) domain proteins, referred to as the BPM proteins, are the substrate adaptors of CULIN3-based E3 ubiquitin ligases (to form CUL3BPM E3 ligases) and are stabilized by JAs. These BPM proteins interact with MYC2, MYC3, and MYC4 proteins and target the MYCs, particularly MYC2 and MYC3, for CUL3BPM E3 ligases-mediated proteasome breakdown [56] (Figure 3d). In addition, a MYC2-mediated feedback inhibition of JA signaling was demonstrated in tomato in which MYC2 induced the expression of the genes encoding MYC2-TARGETED BHLH (MTB) 1, 2, and 3 proteins [57]. The MTB proteins, in turn, inhibit the formation of MYC2–MED25 complex and compete with MYC2 for binding to the MYC2-target promoters [57]. Hence, the core JA-signaling pathway is subjected to a diverse array of negative-feedback control possibly to tailor spatial and temporal termination of JA output.

4. Role of JAs in Plant Defense against P. syringae and Some Other Major Hemibiotrophs

The synergism between ET and JA signaling pathways is a requisite for the activation of plant defense against necrotrophic pathogens [39,48,58]. However, such synergism, and JAs themselves, antagonize plant resistance responses to the hemibiotrophic pathogens, specifically P. syringae either by meddling with the plant morpho-physiological traits or expression of plant defense-related genes [7,59,60,61,62]. The tomato JA-INSENSITIVE 1 (JIN1), a homolog of Arabidopsis COI1, has been implicated as a positive regulator of the hemibiotrophic pathogen P. syringae pv. tomato DC3000 (Pto DC3000)-mediated stomatal reopening, which makes plants more vulnerable to this bacterial strain [7,60,62,63,64]. Notably, the ET- or JA-insensitive Arabidopsis mutants display a drastic reduction in the expression of plant defense-related genes (such as PDF1.2) and consequently exhibit enhanced resistance against P. syringae [58,64,65]. The most predominant plant resistance against hemibiotrophic pathogen depends on the antagonism between JAs and SA, where plants respond to the infecting hemibiotrophic pathogen by activating the SA-dependent defense signaling while suppressing the JA-dependent defense responses [66]. For example, an Arabidopsis coi1 mutant displays enhanced SA levels and transcript level of the SA-inducible PATHOGENESIS-RELATED PROTEIN 1 (PR1) gene, as well as increased resistance to Pto DC3000 infection [67].

4.1. JAs Inhibit Plant Immunity

The JAZ repressors are important mediators of early basal and subsequent secondary plant defense responses [64,68]. For example, the Arabidopsis jaz5/10 double mutant plants display aggravated chlorotic symptoms with increased growth of P. syringae, suggesting that both JAZ5 and JAZ10 mutually function to restrict P. syringae proliferation [68]. Another study reported that JAZ2 is stably expressed in guard cells and regulates stomatal aperture upon P. syringae infection [64]. Arabidopsis jaz2 mutant plants are partially impaired in a microbe-associated molecular pattern (MAMP), from the crude extract of Pto DC3000-induced stomatal closure, and are susceptible to Pto DC3000 [64]. Furthermore, it was demonstrated in this study that Pto DC3000 produced a phytotoxic virulence factor coronatine (COR), which led to the degradation of JAZ2 and the release of JAZ-repressed MYC2 functioning [64].
In addition, the JA signaling regulates plant response to invading P. syringae by inhibiting SA-dependent responses. The suppressive effects of JAs on SA biosynthesis or signaling are mostly mediated by the MYC2. Reportedly, MYC2 represses SA signaling in Arabidopsis as evidenced by the fact that myc2 mutants exhibit increased SA levels, upregulated PR1 expression, and enhanced resistance to Pto DC3000, compared with wild-type plants [67]. Similarly, the deletion of the JA receptor COI1 or MYC2 results in the enhanced levels of endogenous SA and improved resistance to P. syringae [62]. MYC2 binds to the promoters of genes encoding NO APICAL MERISTEM (NAM), Arabidopsis thaliana ACTIVATING FACTOR 1/2 (ATAF1/2), and CUP-SHAPED COTYLEDON 2 (CUC2) domain NAC TFs, such as ANAC019, ANAC055, and ANAC072 in Arabidopsis, and the two homologous NAC TFs JASMONIC ACID 2 (JA2) and JA2-LIKE (JA2L) in tomato, and activate their transcription [61,63,69]. These NAC TFs, in turn, repress the transcription of the SA biosynthesis-related gene SALICYLIC ACID INDUCTION DEFICIENT 2 (SID2, encodes an ISOCHORISMATE SYNTHASE, ICS) but induce the transcription of BENZOIC ACID/SALICYLIC ACID CARBOXYL METHYLTRANSFERASE 1 (BSMT1) that encodes an SA-methylating enzyme. This reprogramming of transcriptional regulation leads to reduced SA accumulation, activation of stomatal reopening, and increased in planta proliferation of P. syringae [61,69]. In addition, EIN3 and EIL1 TFs, which mediate the ERF branch-dependent JA responses (as discussed above in Section 3.2), directly bind to the promoter of the SID2 gene and repress its transcription to negatively regulate Arabidopsis resistance against Pto DC3000 [65]. Moreover, the synergism between ET- and JA-signaling pathways counteracts the suppressive effects of SA on JA signaling through ORA59 [70]. Thus, it is clear that both MYC and ERF branches regulate the JA-mediated responses to Pto DC3000. In addition to MYC2 and EIN3–EIL1, MITOGEN-ACTIVATED PROTEIN (MAP) KINASE 4 (MPK4) is another node that governs the antagonism between JAs and SA, while positively regulating JA signaling but suppressing the SA signaling [71,72]. The Arabidopsis mpk4 mutant plants exhibit impaired expression of JA-responsive PDF1.2 and are insensitive to JAs; however, these mutant plants exhibit increased accumulation of endogenous SA, constitutive expression of SA-dependent PR1 gene, and consequently are more resistant to P. syringae [71,72].

4.2. JAs Positively Mediate Plant Immunity

Increasing evidence is also available for the positive role of JA-mediated signaling in regulating plant immunity. For example, the JA-deficient tomato defenseless-1 (def-1) mutant plants displayed enhanced susceptibility to the Pto DCT6D1 and Xanthomonas campestris pv. versicatoria DC93-1 [73]. The plant cells exhibit an evolutionarily conserved mechanism to sense and respond to the specific pathogens through the virulence factors (or effectors) released by these pathogens during infection, and convert it to robust defense referred to as the effector-triggered immunity (ETI) [74]. More studies have shown that in addition to the increase in endogenous SA levels, the JA concentration also spikes during ETI [75]. Moreover, a plant infected with Pto DC3000 carrying the avrRpt2 (a type III effector gene encoding a cysteine protease) [66], which causes ETI in the infected sites, did not exhibit the antagonism between JA and SA. Furthermore, during Pto DC3000 avrRpt2-mediated ETI induction, although the SA accumulates to a very high level, such accumulation does not lead to the inhibition of JA signaling, however [66]. The SA receptors NONEXPRESSER OF PR GENES 1-LIKE PROTEIN 3 (NPR3) and NPR4 positively regulate JA-dependent defense responses, further resulting in activation of the RESISTANT TO P. syringae 2 (RPS2)-mediated ETI [10]. In the presence of SA, NPR3 and NPR4 instigate the proteasome-mediated breakdown of JAZ repressors, thereby activating the JA-responsive genes [10].
Not only this, but the relationship between JAs and SA during avirulent P. syringae infection also relies on the intracellular ratio of these two hormones, and both JA- and SA-dependent signaling pathways can potentially coexist during a hemibiotrophic infection [76]. For example, the co-treatment of JA and SA at low concentrations leads to the activation of both JA-dependent (e.g., PDF1.2) and SA-dependent (e.g., PR1) signaling [76]. Similarly, treatment with the metabolic elicitors extracted from P. fluorescens N21.4 in Arabidopsis activated the expression of genes associated with both JA (e.g., LOX2 and PDF1.2) and SA (e.g., ICS1, PR1, and PR2) pathways as well as quenched the reactive oxygen species (ROS)-mediated oxidative stress and provided protection against Pto DC3000 [77]. The MED16 (also known as SENSITIVE TO FREEZING 16, SFR16) subunit of the MEDIATOR complex is another potential candidate that mediates the coexistence of JA- and SA-signaling pathways, where it positively regulates both the SA-dependent plant resistance against the P. syringae as well as the JA-dependent resistance responses against the necrotrophic fungal pathogens Alternaria brassicicola and Botrytis cinerea [78].
JAs not only regulate SA biosynthesis and signaling but also influence the intracellular homeostasis of SA. For example, the MeJA application led to the activation of MYC2-dependent signaling and enhanced the expression of ENHANCED DISEASE SUSCEPTIBILITY 5 (EDS5, also known as SID1), encoding a member of the MULTIDRUG AND TOXIN EXTRUSION (MATE) transporter family, which transports SA synthesized in the chloroplasts to the cytoplasm, and reduced expression of PHYTOALEXIN DEFICIENT 4 (PAD4) in a MYC2-dependent manner [79,80,81]. Notably, PAD4 is a positive regulator of EDS5. Thus, JAs inhibit endogenous SA concentration by reducing the expression of PAD4. However, in the case of loss-of-function of PAD4 (i.e., pad4 mutant), JAs positively regulate EDS5 and stimulate SA accumulation [81]. Therefore, JAs may negatively regulate SA-dependent plant immunity against P. syringae in the presence of PAD4 but positively regulate plant defense responses in the absence of PAD4 [81].
In another example, the Arabidopsis constitutive expression of vsp1 (cev1) mutant, which displays a constitutive expression of the JA-dependent ERF branch-regulated PDF1.2 gene, is also more resistant to P. syringae pv. maculicola ES4326 [82]. In addition, the B. cinerea or A. brassicicola infection in Arabidopsis plants led to the activation of both JA- and ET-signaling pathways [70]. In this case, the induction of the JA- and ET-signaling pathways before the exogenous application of SA rendered the plants to be insensitive to the SA-mediated suppression of JA signaling [70]. In Arabidopsis, the functional NPR1 is required for SA-mediated suppression of JA-dependent defenses [70,83]. However, the pharmacological evidence with the application of ET and the ET precursor 1-aminocyclopropane-1-carboxylic acid revealed that ET not only strengthened SA-NPR1-dependent defense responses but also repressed the inhibitory effects of SA on JA-induced PDF1.2 and VSP2 expression [83]. It is thus clear that, in addition to the relative levels of the endogenous JAs and SA, the order and chronology of activation of ET-, JA-, and SA-signaling pathways are also crucial factors determining the plant defense responses under the JA and SA crosstalk during pathogen infection.

5. P. syringae Hijacks JA Signaling to Counter Plant Defense

While plants are well equipped with defense mechanisms to thwart away pathogens, it has also been demonstrated that pathogens employ a cocktail of virulence factors and effectors to manipulate plant defense responses for their own benefits [8,64,84,85]. One of the ways by which biotrophic and hemibiotrophic pathogens make their way into the plant cells is activating host plant JA biosynthesis and/or signaling pathways (Figure 4), which gives them an edge over plant defense machinery during infection by weakening SA-dependent responses [84]. For example, Pto DC3000 secretes COR and hijacks JA signaling. COR is a mimic of the bioactive JA-Ile and perceived through the COI1-JAZ co-receptor complex, it directs JAZ proteins, such as JAZ2, JAZ5, and JAZ10, for degradation (Figure 4a). As a result, the MYC branch is activated, which in turn, induces the expression of NAC family genes (such as ANAC019, ANAC055, and ANAC072). These NAC TFs, in turn, inhibit SA accumulation and SA-dependent plant resistance responses to facilitate the bacterial infection in the host plants [67,69,86]. Pto DC3000 thus hijacks the COI1–JAZ2–MYC2/3/4–ANAC module that is involved in the regulation of stomatal aperture during the infection process [64]. Besides, COR also impedes stomatal closure by suppressing the guard cell-specific NADPH oxidase-dependent ROS production [68]. As opposed to the COI1-dependent functioning of COR, effector HopX1, which encodes a cysteine protease from P. syringae pv. tabaci 11528, interacts with the ZIM domain of all the members of the JAZ protein family and triggers their breakdown in a COI1-independent manner to activate the JA signaling, favoring P. syringae infection [87] (Figure 4b). Additionally, Yang et al. [85] demonstrated that the P. syringae type III effector HopBB1 interacts with both TEOSINTE BRANCHED, CYCLOIDEA, AND PROLIFERATING CELL FACTORS 14 (TCP14), a negative regulator of JA signaling, and JAZ3, a repressor component of JA signaling, and glues them together to facilitate their degradation in a COI1-dependent manner. The breakdown of JAZ3 and TCP14 thus activates the JA-signaling pathway that aids bacterial pathogenesis (Figure 4c, (i)). HopZ1a is another effector molecule produced by P. syringae pv. syringae A2 (Psy) and is a member of a widely distributed YopJ effector family of acetyltransferase. Upon Pto DC3000 infection in Arabidopsis, HopZ1a interacts with JAZ proteins (e.g., AtJAZ2, AtJAZ5, AtJAZ6, AtJAZ8, and AtJAZ12 in Arabidopsis) through the conserved C-terminal Jas domain to acetylate JAZ proteins and direct their degradation in a COI1-dependent manner [8] (Figure 4c, (ii)). The activation of JA-signaling by both HopZ1a and HopX1 led to the suppression of SA-dependent defense responses and increased P. syringae virulence. Both HopBB1 or HopZ1a could partially complement the virulence defects of a COR-deficient P. syringae mutant [8,85], whereas HopX1 could completely rescue the virulence defects of COR-deficiency in Pto DC3000 mutant.
P. syringae secretes another effector molecule, AvrB. This effector binds to RESISTANCE TO P. syringae pv. maculicola 1 (RPM1)-INTERACTING 4 (RIN4) to activate Arabidopsis H(+)-ATPase (AHA1), a plasma membrane-localized proton ATPase (also known as OPEN STOMATA 2, OST2), which results in changes in membrane potential. Alteration in plasma membrane potential increases guard cell turgor pressure to induce stomatal opening [88]. The AvrB-mediated activation of AHA1 also increases the binding of COI1 to JAZ proteins through an unknown mechanism, causing the degradation of multiple JAZ proteins [88] (Figure 4c, (iii)). This results in the activation of JA signaling and stomatal opening, leading to increased plant susceptibility to P. syringae [88]. In addition to interaction with RIN4, AvrB has also been demonstrated to enhance plant susceptibility to a non-pathogenic strain of Pseudomonas by disrupting JA signaling in an rpm1 mutant background [89]. In addition, AvrB also interacts with MPK4 and recruits HEAT SHOCK PROTEIN 90 (HSP90) through an HSP90 co-chaperone REQUIRED FOR MLA12 RESISTANCE 1 (RAR1) and, thus, targets a RIN4–MPK4–RAR1–HSP90 complex to induce JA signaling [90]. Furthermore, AvrB also interacts with and phosphorylates MPK4 [71], and phosphorylated MPK4 in turn binds to and phosphorylates RIN4. The AvrB-activated JA signaling led to enhanced virulence of the infecting pathogens [90].
With building shreds of evidence, it is now apparent that P. syringae injects several virulence factors or effector molecules to hijack host JA-signaling machinery. These effector molecules target different components of the JA-signaling pathway and mostly are redundant in their function, which provide flexibility to the infecting pathogens and makes their infection successful. However, the mechanisms of multiple effectors in hijacking the JA-signaling pathway of the host plant are not clear yet, but the multiple effectors clearly provide the bacteria with an efficient arsenal to breakdown the host defense.

6. Conclusions and Future Perspectives

JAs are involved in a myriad of biological processes. A diverse range of components of the JA metabolism and JA-dependent signaling have been fairly elucidated; however, their cue-dependent regulatory functions and the links between established regulators and newly discovered components of the JA signaling still need to be explored. The prominent role of JAs in plant immunity is highlighted by the arsenal of effectors and virulence factors used by pathogens to activate the JA signaling pathway in the host plants. Given that the antagonism between JAs and SA is phylogenetically widespread and ancient, the exploitation of this antagonism by the pathogens to manipulate the host plant defense often works against a wide range of plant species. Nonetheless, studies have elaborated on the importance of relative concentrations of the JAs and SA and other spatio-temporal factors influencing the two signaling pathways in deciding the fate of the invading pathogens that are vulnerable to SA-dependent defense (e.g., biotrophs or hemibiotrophs) or those that are generally vulnerable to JA-dependent defense (e.g., necrotrophic pathogens and insects). The literature on JA signaling suggests extensive redundancy among different JAZ protein-mediated signaling modules. JAZ proteins not only regulate distinct TFs and an array of downstream responses based on the nature of the pathogens, the host plant developmental stages, tissues, or cell types, but they are also a major hub for molecular crosstalk and the integration of JAs with other signaling pathways. In addition, given the small number of examples in which most pathogens evolved multiple effectors to target JAZ repressors, these JAZ proteins are the best candidate targets for manipulation to develop better defensive plants. Hence, detailed knowledge of JA signaling opens up exciting opportunities for translational research to improve crop resistance under a multi-attacker situation.

Author Contributions

A.G. wrote the original paper draft, A.G. and M.B. prepared the figures and L.-S.P.T. reviewed and edited the manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Wasternack, C.; Strnad, M. Jasmonates: News on occurrence, biosynthesis, metabolism and action of an ancient group of signaling compounds. Int. J. Mol. Sci. 2018, 19, 2539. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  2. Glazebrook, J. Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annu. Rev. Phytopathol. 2005, 43, 205–227. [Google Scholar] [CrossRef] [PubMed]
  3. Cui, H.; Qiu, J.; Zhou, Y.; Bhandari, D.D.; Zhao, C.; Bautor, J.; Parker, J.E. Antagonism of transcription factor MYC2 by EDS1/PAD4 complexes bolsters salicylic acid defense in Arabidopsis effector-triggered immunity. Mol. Plant. 2018, 11, 1053–1066. [Google Scholar] [CrossRef] [PubMed]
  4. Thomma, B.P.H.J.; Eggermont, K.; Penninckx, I.A.M.A.; Mauch-Mani, B.; Vogelsang, R.; Cammue, B.P.A.; Broekaert, W.F. Separate jasmonate-dependent and salicylate-dependent defense-response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens. Proc. Natl. Acad. Sci. USA 1998, 95, 15107–15111. [Google Scholar] [CrossRef] [Green Version]
  5. Klessig, D.F.; Choi, H.W.; Dempsey, D.M.A. Systemic acquired resistance and salicylic acid: Past, present, and future. Mol. Plant-Microbe Interact. 2018, 31, 871–888. [Google Scholar] [CrossRef] [Green Version]
  6. Yang, J.; Duan, G.; Li, C.; Liu, L.; Han, G.; Zhang, Y.; Wang, C. The crosstalks between jasmonic acid and other plant hormone signaling highlight the involvement of jasmonic acid as a core component in plant response to biotic and abiotic stresses. Front. Plant Sci. 2019, 10, 1–12. [Google Scholar] [CrossRef] [Green Version]
  7. Katsir, L.; Schilmiller, A.L.; Staswick, P.E.; He, S.Y.; Howe, G.A. COI1 is a critical component of a receptor for jasmonate and the bacterial virulence factor coronatine. Proc. Natl. Acad. Sci. USA 2008, 105, 7100–7105. [Google Scholar] [CrossRef] [Green Version]
  8. Jiang, S.; Yao, J.; Ma, K.; Zhou, H.; Song, J.; He, S.Y.; Ma, W. Bacterial effector activates jasmonate signaling by directly targeting JAZ transcriptional repressors. PLoS Pathog. 2013, 9, e1003715. [Google Scholar] [CrossRef] [Green Version]
  9. Sheard, L.B.; Tan, X.; Mao, H.; Withers, J.; Ben-nissan, G.; Hinds, T.R.; Kobayashi, Y.; Hsu, F.; Sharon, M.; Browse, J.; et al. Jasmonate perception by inositol phosphate-potentiated COI1- JAZ co-receptor. Nature 2010, 468, 400–405. [Google Scholar] [CrossRef]
  10. Liu, L.; Sonbol, F.M.; Huot, B.; Gu, Y.; Withers, J.; Mwimba, M.; Yao, J.; He, S.Y.; Dong, X. Salicylic acid receptors activate jasmonic acid signalling through a non-canonical pathway to promote effector-triggered immunity. Nat. Commun. 2016, 7, 13099. [Google Scholar] [CrossRef] [Green Version]
  11. Gupta, A.; Hisano, H.; Hojo, Y.; Matsuura, T.; Ikeda, Y.; Mori, I.C.; Senthil-Kumar, M. Global profiling of phytohormone dynamics during combined drought and pathogen stress in Arabidopsis thaliana reveals ABA and JA as major regulators. Sci. Rep. 2017, 7, 4017. [Google Scholar] [CrossRef] [PubMed]
  12. Vick, B.A.; Zimmerman, D.O.N.C. Biosynthesis of jasmonic acid by several plant species. Plant Physiol. 1984, 75, 458–461. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  13. Guan, L.; Denkert, N.; Eisa, A.; Lehmann, M.; Sjuts, I.; Weiberg, A.; Soll, J.; Meinecke, M.; Schwenkert, S. JASSY, a chloroplast outer membrane protein required for jasmonate biosynthesis. Proc. Natl. Acad. Sci. USA 2019, 116, 10568–10575. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  14. Theodoulou, F.L.; Job, K.; Slocombe, S.P.; Footitt, S.; Holdsworth, M.; Baker, A.; Larson, T.R.; Graham, I.A. Jasmonic acid levels are reduced in COMATOSE ATP-binding cassette transporter mutants. Implications for transport of jasmonate precursors into peroxisomes. Plant Physiol. 2005, 137, 835–840. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  15. Seo, H.S.; Song, J.T.; Cheong, J.; Lee, Y.; Lee, Y.; Hwang, I.; Lee, J.S.; Choi, Y. Do Jasmonic acid carboxyl methyltransferase: A key enzyme for jasmonate-regulated plant responses. Proc. Natl. Acad. Sci. USA 2001, 98, 4788–4793. [Google Scholar] [CrossRef] [Green Version]
  16. Staswick, P.E.; Tiryaki, I. The oxylipin signal jasmonic acid is activated by an enzyme that conjugates it to isoleucine in Arabidopsis. Plant Cell 2004, 16, 2117–2127. [Google Scholar] [CrossRef] [Green Version]
  17. Fonseca, S.; Chico, J.M.; Solano, R. The jasmonate pathway: The ligand, the receptor and the core signalling module. Curr. Opin. Plant Biol. 2009, 12, 539–547. [Google Scholar] [CrossRef]
  18. Chini, A.; Monte, I.; Zamarreño, A.M.; Hamberg, M.; Lassueur, S.; Reymond, P.; Weiss, S.; Stintzi, A.; Schaller, A.; Porzel, A.; et al. An OPR3-independent pathway uses 4,5-didehydrojasmonate for jasmonate synthesis. Nat. Chem. Ecol. 2018, 14, 171–178. [Google Scholar] [CrossRef] [Green Version]
  19. Wasternack, C.; Hause, B. A bypass in jasmonate biosynthesis—The OPR3-independent formation. Trends Plant Sci. 2018, 23, 276–279. [Google Scholar] [CrossRef]
  20. Aubert, Y.; Widemann, E.; Miesch, L.; Pinot, F.; Heitz, T. CYP94-mediated jasmonoyl-isoleucine hormone oxidation shapes jasmonate profiles and attenuates defence responses to Botrytis cinerea infection. J. Exp. Bot. 2015, 66, 3879–3892. [Google Scholar] [CrossRef] [Green Version]
  21. Koo, A.J.K.; Cooke, T.F.; Howe, G.A. Cytochrome P450 CYP94B3 mediates catabolism and inactivation of the plant hormone jasmonoyl-L-isoleucine. Proc. Natl. Acad. Sci. USA 2011, 108, 9298–9303. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  22. Koo, A.J.; Thireault, C.; Zemelis, S.; Poudel, A.N.; Zhang, T.; Kitaoka, N.; Brandizzi, F.; Matsuura, H.; Howe, G.A. Endoplasmic reticulum-associated inactivation of the hormone jasmonoyl-L-isoleucine by multiple members of the cytochrome P450 94 family in Arabidopsis. J. Biol. Chem. 2014, 289, 29728–29738. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  23. Heitz, T.; Widemann, E.; Lugan, R.; Miesch, L.; Ullmann, P.; Désaubry, L.; Holder, E.; Grausem, B.; Kandel, S.; Miesch, M.; et al. Cytochromes P450 CYP94C1 and CYP94B3 catalyze two successive oxidation steps of plant hormone jasmonoyl-isoleucine for catabolic turnover. J. Biol. Chem. 2012, 287, 6296–6306. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  24. Bruckhoff, V.; Haroth, S.; Feussner, K.; König, S.; Brodhun, F.; Feussner, I. Functional characterization of CYP94-genes and identification of a novel jasmonate catabolite in flowers. PLoS ONE 2016, 11, 1–26. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  25. Kitaoka, N.; Matsubara, T.; Sato, M.; Takahashi, K.; Wakuta, S.; Kawaide, H.; Matsui, H.; Nabeta, K.; Matsuura, H. Arabidopsis CYP94B3 encodes jasmonyl-l-isoleucine 12-hydroxylase, a key enzyme in the oxidative catabolism of jasmonate. Plant Cell Physiol. 2011, 52, 1757–1765. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  26. Marquis, V.; Smirnova, E.; Poirier, L.; Zumsteg, J.; Schweizer, F.; Reymond, P.; Heitz, T. Stress- and pathway-specific impacts of impaired jasmonoyl-isoleucine (JA-Ile) catabolism on defense signalling and biotic stress resistance. Plant Cell Environ. 2020, 1–13. [Google Scholar] [CrossRef]
  27. Caarls, L.; Elberse, J.; Awwanah, M.; Ludwig, N.R.; De Vries, M.; Zeilmaker, T.; Van Wees, S.C.M.; Schuurink, R.C.; Van den Ackerveken, G. Arabidopsis JASMONATE-INDUCED OXYGENASES down-regulate plant immunity by hydroxylation and inactivation of the hormone jasmonic acid. Proc. Natl. Acad. Sci. USA 2017, 114, 6388–6393. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  28. Widemann, E.; Miesch, L.; Lugan, R.; Holder, E.; Heinrich, C.; Aubert, Y.; Miesch, M.; Pinot, F.; Heitz, T. The amidohydrolases IAR3 and ILL6 contribute to jasmonoyl-isoleucine hormone turnover and generate 12-hydroxyjasmonic acid upon wounding in Arabidopsis. J. Biol. Chem. 2013, 288, 31701–31714. [Google Scholar] [CrossRef] [Green Version]
  29. Chini, A.; Fonseca, S.; Fernández, G.; Adie, B.; Chico, J.M.; Lorenzo, O.; García-Casado, G.; López-Vidriero, I.; Lozano, F.M.; Ponce, M.R.; et al. The JAZ family of repressors is the missing link in jasmonate signalling. Nature 2007, 448, 666–671. [Google Scholar] [CrossRef]
  30. Thines, B.; Katsir, L.; Melotto, M.; Niu, Y.; Mandaokar, A.; Liu, G.; Nomura, K.; He, S.Y.; Howe, G.A.; Browse, J. JAZ repressor proteins are targets of the SCF COI1 complex during jasmonate signalling. Nature 2007, 448, 661–665. [Google Scholar] [CrossRef]
  31. Wasternack, C.; Song, S. Jasmonates: Biosynthesis, metabolism, and signaling by proteins activating and repressing transcription. J. Exp. Bot. 2017, 68, 1303–1321. [Google Scholar] [CrossRef] [PubMed]
  32. Pauwels, L.; Fernández Barbero, G.; Geerinck, J.; Tilleman, S.; Grunewald, W.; Pérez, A.C.; Chico, J.M.; Bossche, R.V.; Sewell, J.; Gil, E.; et al. NINJA connects the co-repressor TOPLESS to jasmonate signalling. Nature 2010, 464, 788–791. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  33. Wasternack, C.; Hause, B. Jasmonates: Biosynthesis, perception, signal transduction and action in plant stress response, growth and development. An update to the 2007 review in Annals of Botany. Ann. Bot. 2013, 111, 1021–1058. [Google Scholar] [CrossRef] [PubMed]
  34. Zhu, Z.; An, F.; Feng, Y.; Li, P.; Xue, L.; A, M.; Jiang, Z.; Kim, J.-M.; To, T.K.; Li, W.; et al. Derepression of ethylene-stabilized transcription factors (EIN3/EIL1) mediates jasmonate and ethylene signaling synergy in Arabidopsis. Proc. Natl. Acad. Sci. USA 2011, 108, 12539–12544. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  35. Wu, K.; Zhang, L.; Zhou, C.; Yu, C.; Chaikam, V. HDA6 is required for jasmonate response, senescence and flowering in Arabidopsis. J. Exp. Bot. 2008, 59, 225–234. [Google Scholar] [CrossRef]
  36. Li, Q.; Zheng, J.; Li, S.; Huang, G.; Skilling, S.J.; Wang, L.; Li, L.; Li, M.; Yuan, L.; Liu, P. Transporter-mediated nuclear entry of jasmonoyl-isoleucine is essential for jasmonate signaling. Mol. Plant 2017, 10, 695–708. [Google Scholar] [CrossRef] [Green Version]
  37. Mosblech, A.; Thurow, C.; Gatz, C.; Feussner, I.; Heilmann, I.; Karspu, U. Jasmonic acid perception by COI1 involves inositol polyphosphates in Arabidopsis thaliana. Plant J. 2011, 65, 949–957. [Google Scholar] [CrossRef]
  38. Chen, R.; Jiang, H.; Li, L.; Zhai, Q.; Qi, L.; Zhou, W.; Liu, X.; Li, H.; Zheng, W.; Sun, J.; et al. The Arabidopsis mediator subunit MED25 differentially regulates jasmonate and abscisic acid signaling through interacting with the MYC2 and ABI5 transcription factors. Plant Cell 2012, 24, 2898–2916. [Google Scholar] [CrossRef] [Green Version]
  39. Zarei, A.; Korbes, A.P.; Younessi, P.; Montiel, G.; Champion, A.; Memelink, J. Two GCC boxes and AP2/ERF-domain transcription factor ORA59 in jasmonate/ethylene-mediated activation of the PDF1.2 promoter in Arabidopsis. Plant Mol. Biol. 2011, 75, 321–331. [Google Scholar] [CrossRef] [Green Version]
  40. Lorenzo, O.; Chico, J.M.; Sa, J.J. JASMONATE-INSENSITIVE1 encodes a MYC transcription factor essential to discriminate between different jasmonate-regulated defense responses in Arabidopsis. Plant Cell 2004, 16, 1938–1950. [Google Scholar] [CrossRef] [Green Version]
  41. Lorenzo, O.; Solano, R. Molecular players regulating the jasmonate signalling network. Curr. Opin. Plant Biol. 2005, 8, 532–540. [Google Scholar] [CrossRef] [PubMed]
  42. Verhage, A.; Vlaardingerbroek, I.; Raaymakers, C.; Van Dam, N.M.; Dicke, M.; Van Wees, S.C.M.; Pieterse, C.M.J. Rewiring of the jasmonate signaling pathway in Arabidopsis during insect herbivory. Front. Plant Sci. 2011, 2, 47. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  43. Song, S.; Huang, H.; Gao, H.; Wang, J.; Wu, D.; Liu, X.; Yang, S.; Zhai, Q.; Li, C.; Qi, T.; et al. Interaction between MYC2 and ETHYLENE INSENSITIVE3 modulates antagonism between jasmonate and ethylene signaling in Arabidopsis. Plant Cell 2014, 26, 263–279. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  44. Fernandez-Calvo, P.; Chini, A.; Fernandez-Barbero, G.; Chico, J.-M.; Gimenez-ibanez, S.; Geerinck, J.; Eeckhout, D.; Schweizer, F.; Godoy, M.; Franco-zorrilla, J.M.; et al. The Arabidopsis bHLH transcription factors MYC3 and MYC4 are targets of JAZ repressors and act additively with MYC2 in the activation of jasmonate responses. Plant Cell 2011, 23, 701–715. [Google Scholar] [CrossRef] [Green Version]
  45. Kidd, B.N.; Edgar, C.I.; Kumar, K.K.; Aitken, E.A.; Schenk, P.M.; Manners, J.M.; Kazan, K. The mediator complex subunit PFT1 is a key regulator of jasmonate-dependent defense in Arabidopsis. Plant Cell 2009, 21, 2237–2252. [Google Scholar] [CrossRef] [Green Version]
  46. An, C.; Li, L.; Zhai, Q.; You, Y.; Deng, L.; Wu, F.; Chen, R.; Jiang, H. Mediator subunit MED25 links the jasmonate receptor to transcriptionally active chromatin. Proc. Natl. Acad. Sci. USA 2017, E8930–E8939. [Google Scholar] [CrossRef] [Green Version]
  47. Zhang, F.; Yao, J.; Ke, J.; Zhang, L.; Lam, V.Q.; Xin, X.-F.; Zhou, X.E.; Chen, J.; Brunzelle, J.; Griffin, P.R.; et al. Structural basis of JAZ repression of MYC transcription factors in jasmonate signaling. Nature 2015, 344, 1173–1178. [Google Scholar] [CrossRef]
  48. Broekaert, W.F.; Delaur, S.L.; De Bolle, M.F.C.; Cammue, B.P.A. The role of ethylene in host-pathogen interactions. Annu. Rev. Phytopathol. 2006, 44, 393–416. [Google Scholar] [CrossRef]
  49. Zhou, C.; Zhang, L.; Duan, J.; Miki, B.; Wu, K. HISTONE DEACETYLASE19 is involved in jasmonic acid and ethylene signaling of pathogen response in Arabidopsis. Plant Cell 2005, 17, 1196–1204. [Google Scholar] [CrossRef] [Green Version]
  50. Çevik, V.; Kidd, B.N.; Zhang, P.; Hill, C.; Kiddle, S.; Denby, K.J.; Holub, E.B.; Cahill, D.M.; Manners, J.M.; Schenk, P.M.; et al. MEDIATOR25 acts as an integrative hub for the regulation of jasmonate-responsive gene expression in Arabidopsis. Plant Physiol. 2012, 160, 541–555. [Google Scholar] [CrossRef] [Green Version]
  51. Sasaki-Sekimoto, Y.; Jikumaru, Y.; Obayashi, T.; Saito, H.; Masuda, S.; Kamiya, Y.; Ohta, H.; Shirasu, K. Basic helix-loop-helix transcription factors JASMONATE-ASSOCIATED MYC2-LIKE1 (JAM1), JAM2, and JAM3 are negative regulators of jasmonate responses in Arabidopsis. Plant Physiol. 2013, 163, 291–304. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  52. Sasaki-Sekimoto, Y.; Saito, H.; Masuda, S.; Shirasu, K.; Ohta, H. Comprehensive analysis of protein interactions between JAZ proteins and bHLH transcription factors that negatively regulate jasmonate signaling. Plant Signal. Behav. 2014, 9. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  53. Yan, C.; Fan, M.; Yang, M.; Zhao, J.; Zhang, W.; Su, Y.; Xiao, L.; Deng, H.; Xie, D. Injury activates Ca2+/Calmodulin-dependent phosphorylation of JAV1-JAZ8-WRKY51 complex for jasmonate biosynthesis. Mol. Cell 2018, 70, 136–149.e7. [Google Scholar] [CrossRef] [Green Version]
  54. Jung, C.; Zhao, P.; Seo, J.S.; Mitsuda, N.; Deng, S.; Chua, N.H. PLANT U-BOX PROTEIN10 regulates MYC2 stability in Arabidopsis. Plant Cell 2015, 27, 2016–2031. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  55. Jeong, J.S.; Jung, C.; Seo, J.S.; Kim, J.K.; Chua, N.H. The deubiquitinating enzymes UBP12 and UBP13 positively regulate MYC2 levels in jasmonate responses. Plant Cell 2017, 29, 1406–1424. [Google Scholar] [CrossRef] [Green Version]
  56. Chico, J.M.; Lechner, E.; Fernandez-Barbero, G.; Canibano, E.; García-Casado, G.; Franco-Zorrilla, J.M.; Hammann, P.; Zamarreño, A.M.; García-Mina, J.M.; Rubio, V.; et al. CUL3BPM E3 ubiquitin ligases regulate MYC2, MYC3, and MYC4 stability and JA responses. Proc. Natl. Acad. Sci. USA 2020, 117, 6205–6215. [Google Scholar] [CrossRef]
  57. Liu, Y.; Du, M.; Deng, L.; Shen, J.; Fang, M.; Chen, Q.; Lu, Y.; Wang, Q.; Li, C.; Zhai, Q. MYC2 regulates the termination of jasmonate signaling via an autoregulatory negative feedback loop. Plant Cell 2019, 31, 106–127. [Google Scholar] [CrossRef] [Green Version]
  58. Penninckx, I.A.M.A.; Thomma, B.P.H.J.; Buchala, A.; Métraux, J.P.; Broekaert, W.F. Concomitant activation of jasmonate and ethylene response pathways is required for induction of a plant defensin gene in Arabidopsis. Plant Cell 1998, 10, 2103–2113. [Google Scholar] [CrossRef] [Green Version]
  59. Gimenez-Ibanez, S.; Chini, A.; Solano, R. How microbes twist jasmonate signaling around their little fingers. Plants 2016, 5, 9. [Google Scholar] [CrossRef] [Green Version]
  60. Feys, B.J.F.; Benedetti, C.E.; Penfold, C.N.; Turner, J.G. Arabidopsis mutants selected for resistance to the phytotoxin coronatine are male sterile, insensitive to methyl jasmonate, and resistant to a bacterial pathogen. Plant Cell 1994, 6, 751–759. [Google Scholar] [CrossRef] [Green Version]
  61. Du, M.; Zhai, Q.; Deng, L.; Li, S.; Li, H.; Yan, L.; Huang, Z.; Wang, B.; Jiang, H.; Huang, T.; et al. Closely related NAC transcription factors of tomato differentially regulate stomatal closure and reopening during pathogen attack. Plant Cell 2014, 26, 3167–3184. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  62. Kloek, A.P.; Verbsky, M.L.; Sharma, S.B.; Schoelz, J.E.; Vogel, J.; Klessig, D.F.; Kunkel, B.N. Resistance to Pseudomonas syringae conferred by an Arabidopsis thaliana coronatine-insensitive (coi1) mutation occurs through two distinct mechanisms. Plant J. 2001, 26, 509–522. [Google Scholar] [CrossRef] [PubMed]
  63. Du, M.; Zhao, J.; Tzeng, D.T.W.W.; Liu, Y.; Deng, L.; Yang, T.; Zhai, Q.; Wu, F.; Huang, Z.; Zhou, M.; et al. MYC2 orchestrates a hierarchical transcriptional cascade that regulates jasmonate-mediated plant immunity in tomato. Plant Cell 2017, 29, 1883–1906. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  64. Gimenez-ibanez, S.; Boter, M.; Ortigosa, A.; Garcia-Casado, G.; Chini, A.; Lewsey, M.G.; Ecker, J.R.; Ntoukakis, V.; Solano, R. JAZ2 controls stomata dynamics during bacterial invasion. New Phytol. 2017, 213, 1378–1392. [Google Scholar] [CrossRef] [Green Version]
  65. Chen, H.; Xue, L.; Chintamanani, S.; Germain, H.; Lin, H.; Cui, H.; Cai, R.; Zuo, J.; Tang, X.; Li, X.; et al. ETHYLENE INSENSITIVE3 and ETHYLENE INSENSITIVE3-LIKE1 repress SALICYLIC ACID INDUCTION DEFICIENT2 expression to negatively regulate plant innate immunity in Arabidopsis. Plant Cell 2009, 21, 2527–2540. [Google Scholar] [CrossRef] [Green Version]
  66. Spoel, S.H.; Johnson, J.S.; Dong, X. Regulation of tradeoffs between plant defenses against pathogens with different lifestyles. Proc. Natl. Acad. Sci. USA 2007, 104, 18842–18847. [Google Scholar] [CrossRef] [Green Version]
  67. Laurie-berry, N.; Joardar, V.; Street, I.H.; Kunkel, B.N.; Box, C.; Drive, B.; Louis, S. The Arabidopsis thaliana JASMONATE INSENSITIVE 1 gene is required for suppression of salicylic acid-dependent defenses during infection by Pseudomonas syringae. Mol. Plant-Microbe Interact. 2006, 19, 789–800. [Google Scholar] [CrossRef] [Green Version]
  68. Toum, L.; Torres, P.S.; Gallego, S.M.; Benavídes, M.P.; Vojnov, A.A.; Gudesblat, G.E. Coronatine inhibits stomatal closure through guard cell-specific inhibition of NADPH oxidase-dependent ROS production. Front. Plant Sci. 2016, 7, 1851. [Google Scholar] [CrossRef] [Green Version]
  69. Zheng, X.; Spivey, N.W.; Zeng, W.; Liu, P.-P.; Fu, Z.Q.; Klessig, D.F.; He, S.Y.; Dong, X. Coronatine promotes Pseudomonas syringae virulence in plants by activating a signaling cascade that inhibits salicylic acid accumulation. Cell Host Microbe 2012, 11, 587–596. [Google Scholar] [CrossRef] [Green Version]
  70. Leon-Reyes, A.; Du, Y.; Koornneef, A.; Proietti, S.; Körbes, A.P.; Memelink, J.; Pieterse, C.M.J.; Ritsema, T. Ethylene signaling renders the jasmonate response of Arabidopsis insensitive to future suppression by salicylic acid. Mol. Plant-Microbe Interact. 2010, 23, 187–197. [Google Scholar] [CrossRef] [Green Version]
  71. Petersen, M.; Brodersen, P.; Naested, H.; Andreasson, E.; Lindhart, U.; Johansen, B.; Nielsen, H.B.; Lacy, M.; Austin, M.J.; Parker, J.E.; et al. Arabidopsis MAP kinase 4 negatively regulates systemic acquired resistance. Cell 2000, 103, 1111–1120. [Google Scholar] [CrossRef] [Green Version]
  72. Brodersen, P.; Petersen, M.; Nielsen, H.B.; Zhu, S.; Newman, M.A.; Shokat, K.M.; Rietz, S.; Parker, J.; Mundy, J. Arabidopsis MAP kinase 4 regulates salicylic acid- and jasmonic acid/ethylene-dependent responses via EDS1 and PAD4. Plant J. 2006, 47, 532–546. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  73. Thaler, J.S.; Owen, B.; Higgins, V.J. The role of the jasmonate response in plant susceptibility to diverse pathogens with a range of lifestyles. Plant Physiol. 2004, 135, 530–538. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  74. Cui, H.; Tsuda, K.; Parker, J.E. Effector-triggered immunity: From pathogen perception to robust defense. Annu. Rev. Plant Biol. 2015, 66, 487–511. [Google Scholar] [CrossRef]
  75. Kenton, P.; Mur, L.A.J.; Atzorn, R.; Wasternack, C.; Draper, J. (−)-Jasmonic acid accumulation in tobacco hypersensitive response lesions. Mol. Plant-Microbe Interact. 1999, 12, 74–78. [Google Scholar] [CrossRef] [Green Version]
  76. Mur, L.A.J.; Kenton, P.; Atzorn, R.; Miersch, O.; Wasternack, C. The outcomes of concentration-specific interactions between salicylate and jasmonate signaling include synergy, antagonism, and oxidative stress leading to cell death. Plant Physiol. 2006, 140, 249–262. [Google Scholar] [CrossRef] [Green Version]
  77. Martin-Rivilla, H.; Garcia-Villaraco, A.; Ramos-Solano, B.; Gutierrez-Mañero, F.J.; Lucas, J.A. Extracts from cultures of Pseudomonas fluorescens induce defensive patterns of gene expression and enzyme activity while depressing visible injury and reactive oxygen species in Arabidopsis thaliana challenged with pathogenic Pseudomonas. AoB Plants 2019, 11, 1–26. [Google Scholar] [CrossRef]
  78. Zhang, X.; Wang, C.; Zhang, Y.; Sun, Y.; Mou, Z. The Arabidopsis mediator complex subunit16 positively regulates salicylate-mediated systemic acquired resistance and jasmonate/ethylene-induced defense pathways. Plant Cell 2012, 24, 4294–4309. [Google Scholar] [CrossRef] [Green Version]
  79. Nawrath, C.; Heck, S.; Parinthawong, N.; Métraux, J.P. EDS5, an essential component of salicylic acid-dependent signaling for disease resistance in Arabidopsis, is a member of the MATE transporter family. Plant Cell 2002, 14, 275–286. [Google Scholar] [CrossRef] [Green Version]
  80. Serrano, M.; Wang, B.; Aryal, B.; Garcion, C.; Abou-Mansour, E.; Heck, S.; Geisler, M.; Mauch, F.; Nawrath, C.; Métraux, J.P. Export of salicylic acid from the chloroplast requires the multidrug and toxin extrusion-like transporter EDS5. Plant Physiol. 2013, 162, 1815–1821. [Google Scholar] [CrossRef] [Green Version]
  81. Mine, A.; Nobori, T.; Salazar-rondon, M.C.; Winkelmüller, T.M.; Anver, S.; Becker, D.; Tsuda, K. An incoherent feed-forward loop mediates robustness and tunability in a plant immune network. EMBO Rep. 2017, 18, 464–476. [Google Scholar] [CrossRef] [PubMed]
  82. Ellis, C.; Karafyllidis, I.; Turner, J.G. Constitutive activation of jasmonate signaling in an Arabidopsis mutant correlates with enhanced resistance to Erysiphe cichoracearum, Pseudomonas syringae, and Myzus persicae. Mol. Plant-Microbe Interact. 2002, 15, 1025–1030. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  83. Leon-Reyes, A.; Spoel, S.H.; De Lange, E.S.; Abe, H.; Kobayashi, M.; Tsuda, S.; Millenaar, F.F.; Welschen, R.A.M.; Ritsema, T.; Pieterse, C.M.J. Ethylene modulates the role of NONEXPRESSOR OF PATHOGENESIS-RELATED GENES1 in cross talk between salicylate and jasmonate signaling. Plant Physiol. 2009, 149, 1797–1809. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  84. Uppalapati, S.R.; Ishiga, Y.; Wangdi, T.; Kunkel, B.N.; Anand, A.; Mysore, K.S.; Bender, C.L. The phytotoxin coronatine contributes to pathogen fitness and is required for suppression of salicylic acid accumulation in tomato inoculated with Pseudomonas syringae pv. tomato DC3000. Mol. Plant-Microbe Interact. 2007, 20, 955–965. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  85. Yang, L.; Teixeira, P.J.; Biswas, S.; Finkel, O.M.; He, Y.; Salas-Gonzalez, I.; English, M.E.; Epple, P.; Mieczkowski, P.; Dangl, J.L. Pseudomonas syringae type III effector HopBB1 promotes host transcriptional repressor degradation to regulate phytohormone responses and virulence. Cell Host Microbe 2017, 21, 156–168. [Google Scholar] [CrossRef] [Green Version]
  86. Wasternack, C. The Trojan horse coronatine: The COI1–JAZ2–MYC2,3,4–ANAC019,055,072 module in stomata dynamics upon bacterial infection. New Phytol. 2017, 213, 972–975. [Google Scholar] [CrossRef] [Green Version]
  87. Gimenez-Ibanez, S.; Boter, M.; Ferna´ndez-Barbero, G.; Chini, A.; Rathjen, J.P.; Solano, R. The bacterial effector HopX1 targets JAZ transcriptional repressors to activate jasmonate signaling and promote infection in Arabidopsis. PLoS Biol. 2014, 12, e1001792. [Google Scholar] [CrossRef] [Green Version]
  88. Zhou, Z.; Wu, Y.; Yang, Y.; Du, M.; Zhang, X.; Guo, Y.; Li, C.; Zhou, J. An Arabidopsis plasma membrane proton ATPase modulates JA signaling and is exploited by the Pseudomonas syringae effector protein AvrB for stomatal invasion. Plant Cell 2015, 27, 2032–2041. [Google Scholar] [CrossRef] [Green Version]
  89. Shang, Y.; Li, X.; Cui, H.; He, P.; Thilmony, R.; Chintamanani, S.; Zwiesler-Vollick, J.; Gopalan, S.; Tang, X.; Zhou, J.M. RAR1, a central player in plant immunity, is targeted by Pseudomonas syringae effector AvrB. Proc. Natl. Acad. Sci. USA 2006, 103, 19200–19205. [Google Scholar] [CrossRef] [Green Version]
  90. Cui, H.; Wang, Y.; Xue, L.; Chu, J.; Yan, C.; Fu, J.; Chen, M.; Innes, R.W. Pseudomonas syringae effector protein AvrB perturbs Arabidopsis hormone signaling by activating MAP Kinase 4. Cell Host Microbe 2010, 7, 164–175. [Google Scholar] [CrossRef] [Green Version]
Ijms 21 07482 g001 550
Figure 1. Metabolism of jasmonates (JAs). (a) Jasmonic acid (JA) is synthesized from α-linolenic acid by the various enzymes present in chloroplasts (CPs) and peroxisomes (PRs) [12]. LOX, AOS, and AOC are the key enzymes of JA biosynthesis present in CPs. These enzymes convert α-linolenic acid into OPDA through a series of reactions. OPDA is then exported from the CPs by the JASSY transmembrane protein present on the inner membrane of the CPs [13]. OPDA enters into PRs through an ABC transporter membrane protein known as COMATOSE (CTS) [14]. OPDA is directly converted to JA in the PRs with the help of OPR3 and ACX1 enzymes through β-oxidation, and then transported into the cytoplasm (CM) [12]. In the absence of a functional OPR3 enzyme, OPDA is converted to 4,5-ddh-JA, which is then transported into the CM [18,19]. The 4,5-ddh-JA is now converted to JA through the OPR2 enzyme in the CM. In the CM, JAR1 and JMT act on the JA and convert it into JA-Ile [16] and MeJA [15], respectively. (b) JA catabolism is regulated by CYP94B1, CYP94B3, and CYP94C1 present in the endoplasmic reticulum (ER) and amidohydrolases IAR3 and ILL6 [22]. (i) CYP94B1 and CYP94B3 degrade JA-Ile to the weak analog 12OH-JA-Ile, while CYP94B3 and CYP94C1 catalyze JA-Ile to the inactive form 12COOH-JA-Ile [21,22,23,24,25,26] (i). JA-Ile is also converted to JA with the help of IAR3 and ILL6 enzymes [28] (ii). These enzymes also work downstream to the CYP94s and breakdown 12OH-JA-Ile generated from step (i) to 12OH-JA. In an alternative catabolic route, JOX hydroxylates JA to 12OH-JA [27] (iii). ACXI, ACYL-CoA-OXIDASE 1; AOC, ALLENE OXIDE CYCLASE; AOS, ALLENE OXIDE SYNTHASE; 12COOH-JA-Ile, 12-carboxy-jasmonic acid isoleucine; CYP94, CYTOCHROME P450 MONOOXYGENASE CYP94 FAMILY; 4,5-ddh-JA, 4,5-didehydro-jasmonic acid; dnOPDA; dinor-oxo-phytodienoic acid; 12,13-EOT,12,13-epoxyoctadecatrienoic acid; 13-HPOT, 13-hydroperoxyoctadecatrienoic acid; IAR3, INDOLE ACETIC ACID (IAA)-ALANINE RESISTANT 3; ILL6, IAA-LEUCINE RESISTANT (ILR)-LIKE GENE 6; JA-Ile, isoleucine conjugated jasmonate; JAO, JASMONIC ACID OXIDASE; JAR1, JASMONATE RESISTANT 1; JASSY, a membrane channel that is located in the outer envelope of chloroplasts; JMT, S-ADENOSYL-L-METHIONINE: JASMONIC ACID CARBOXYL METHYLTRANSFERASE; LOX, 13-LIPOXYGENASE; MeJA, methyl jasmonate; 12OH-JA, 12-hydroxy-jasmonic acid; 12OH-JA-Ile, 12-hydroxy-jasmonic acid isoleucine; OPC8, 3-oxo-2(2′[Z]-pentenyl)-cyclopentane-1-octanoic acid; OPDA, 12-oxo-phytodienoic acid; OPR, OPDA REDUCTASE; tnOPDA, tetranor-oxo-phytodienoic acid.
Figure 1. Metabolism of jasmonates (JAs). (a) Jasmonic acid (JA) is synthesized from α-linolenic acid by the various enzymes present in chloroplasts (CPs) and peroxisomes (PRs) [12]. LOX, AOS, and AOC are the key enzymes of JA biosynthesis present in CPs. These enzymes convert α-linolenic acid into OPDA through a series of reactions. OPDA is then exported from the CPs by the JASSY transmembrane protein present on the inner membrane of the CPs [13]. OPDA enters into PRs through an ABC transporter membrane protein known as COMATOSE (CTS) [14]. OPDA is directly converted to JA in the PRs with the help of OPR3 and ACX1 enzymes through β-oxidation, and then transported into the cytoplasm (CM) [12]. In the absence of a functional OPR3 enzyme, OPDA is converted to 4,5-ddh-JA, which is then transported into the CM [18,19]. The 4,5-ddh-JA is now converted to JA through the OPR2 enzyme in the CM. In the CM, JAR1 and JMT act on the JA and convert it into JA-Ile [16] and MeJA [15], respectively. (b) JA catabolism is regulated by CYP94B1, CYP94B3, and CYP94C1 present in the endoplasmic reticulum (ER) and amidohydrolases IAR3 and ILL6 [22]. (i) CYP94B1 and CYP94B3 degrade JA-Ile to the weak analog 12OH-JA-Ile, while CYP94B3 and CYP94C1 catalyze JA-Ile to the inactive form 12COOH-JA-Ile [21,22,23,24,25,26] (i). JA-Ile is also converted to JA with the help of IAR3 and ILL6 enzymes [28] (ii). These enzymes also work downstream to the CYP94s and breakdown 12OH-JA-Ile generated from step (i) to 12OH-JA. In an alternative catabolic route, JOX hydroxylates JA to 12OH-JA [27] (iii). ACXI, ACYL-CoA-OXIDASE 1; AOC, ALLENE OXIDE CYCLASE; AOS, ALLENE OXIDE SYNTHASE; 12COOH-JA-Ile, 12-carboxy-jasmonic acid isoleucine; CYP94, CYTOCHROME P450 MONOOXYGENASE CYP94 FAMILY; 4,5-ddh-JA, 4,5-didehydro-jasmonic acid; dnOPDA; dinor-oxo-phytodienoic acid; 12,13-EOT,12,13-epoxyoctadecatrienoic acid; 13-HPOT, 13-hydroperoxyoctadecatrienoic acid; IAR3, INDOLE ACETIC ACID (IAA)-ALANINE RESISTANT 3; ILL6, IAA-LEUCINE RESISTANT (ILR)-LIKE GENE 6; JA-Ile, isoleucine conjugated jasmonate; JAO, JASMONIC ACID OXIDASE; JAR1, JASMONATE RESISTANT 1; JASSY, a membrane channel that is located in the outer envelope of chloroplasts; JMT, S-ADENOSYL-L-METHIONINE: JASMONIC ACID CARBOXYL METHYLTRANSFERASE; LOX, 13-LIPOXYGENASE; MeJA, methyl jasmonate; 12OH-JA, 12-hydroxy-jasmonic acid; 12OH-JA-Ile, 12-hydroxy-jasmonic acid isoleucine; OPC8, 3-oxo-2(2′[Z]-pentenyl)-cyclopentane-1-octanoic acid; OPDA, 12-oxo-phytodienoic acid; OPR, OPDA REDUCTASE; tnOPDA, tetranor-oxo-phytodienoic acid.
Ijms 21 07482 g001
Ijms 21 07482 g002 550
Figure 2. Overview of the jasmonic acid (JA) signaling pathway. (a) In the non-inductive phase of JA signaling or in the absence of jasmonates (JAs), JAZ proteins form a complex with the co-repressor TPL and TPR proteins [31]. The association between JAZs and TPL–TPR co-repressors is mediated by the NINJA adaptor protein [32]. The JAZNINJA–TPL–TPR repressor complex then binds to the transcription factors (TFs, such as MYCs, EIN3, and EIL1) and prevents the induction of JA signaling (i). JAZ proteins also recruit the transcriptional co-repressor HDA6 protein, which deacetylates histones (especially H4) and inhibits EIN3 and EIL1 to bind to the target gene promoters, leading to the subsequent repression of JA-responsive gene transcription [34,35] (ii). (b) When a sufficient level of JA-Ile is present in the cells (induction phase), JA-Ile enters into the nucleus through JAT1 membrane transporter [36] and binds to COI1 and JAZNINJA–TPL–TPR repressor complex, leading to the COI1-mediated polyubiquitination of JAZ. The polyubiquitination tags JAZ proteins for 26S proteasome-mediated breakdown, thereby releasing the TFs and JA-signaling repressors from the complex. Inositol pentakisphosphate (InsP5), a co-receptor for JA-Ile, stabilizes the association of COI1–JAZ complex [37]. Depending upon the nature of infecting pathogens, the JA signaling operates through either (i) MYC or (ii) ERF TFs. The regulation of JA signaling in the MYC2 branch involves the association of HAC1 with MYC2 and MED25 proteins and binding of various MYC TFs [38]. This activator complex then induces the transcription of the downstream JA-responsive gene (e.g., VSP2) (i). The regulation of JA signaling through the ERF branch is mediated by ethylene (ET), EIN3, and EIL1. EIN3 and EIL1 TFs induce the transcription of ERF1 and ORA59. ERF1 and ORA59 TFs, in turn, recruit MED25 to the GCC-box motif in the target gene promoters, leading to the activation of JA-responsive genes (e.g., PDF1.2) [39] (ii). Double-headed bars show mutual repression, while dotted arrows indicate the release of TFs from the repressor complex. L-arrows and L-bars indicate induction and repression of gene transcription, respectively. Crosses indicate the inability of proteins to bind to DNA sequences. COI1, CORONATINE INSENSITIVE 1 receptor; EIL1, ETHYLENE-INSENSITIVE 3-LIKE 1; EIN3, ETHYLENE-INSENSITIVE 3; ERF, ETHYLENE RESPONSE FACTOR; GTFs, general transcription factors; HAC1, HISTONE ACETYLTRANSFERASE OF THE CBP FAMILY 1; HDA, HISTONE DEACETYLASE; InsP5, inositol pentakisphosphate; JA-Ile, isoleucine conjugated jasmonate; JAT1, JASMONATE TRANSPORTER 1; JAZ, JASMONATE ZINC-FINGER EXPRESSED IN INFLORESCENCE MERISTEM (ZIM)-DOMAIN PROTEIN; MED25, a subunit of the MEDIATOR transcriptional co-activator complex; MYC, MYELOCYTOMATOSIS; NINJA, NOVEL INTERACTOR OF JAZ; ORA59, OCTADECANOID-RESPONSIVE ARABIDOPSIS AP2 (APETALA2)/ERF59; PDF1.2, PLANT DEFENSIN 1.2; RNA Pol II, RNA POLYMERASE II; TPL, TOPLESS; TPR, TPL-RELATED proteins; VSP2, VEGETATIVE STORAGE PROTEIN 2.
Figure 2. Overview of the jasmonic acid (JA) signaling pathway. (a) In the non-inductive phase of JA signaling or in the absence of jasmonates (JAs), JAZ proteins form a complex with the co-repressor TPL and TPR proteins [31]. The association between JAZs and TPL–TPR co-repressors is mediated by the NINJA adaptor protein [32]. The JAZNINJA–TPL–TPR repressor complex then binds to the transcription factors (TFs, such as MYCs, EIN3, and EIL1) and prevents the induction of JA signaling (i). JAZ proteins also recruit the transcriptional co-repressor HDA6 protein, which deacetylates histones (especially H4) and inhibits EIN3 and EIL1 to bind to the target gene promoters, leading to the subsequent repression of JA-responsive gene transcription [34,35] (ii). (b) When a sufficient level of JA-Ile is present in the cells (induction phase), JA-Ile enters into the nucleus through JAT1 membrane transporter [36] and binds to COI1 and JAZNINJA–TPL–TPR repressor complex, leading to the COI1-mediated polyubiquitination of JAZ. The polyubiquitination tags JAZ proteins for 26S proteasome-mediated breakdown, thereby releasing the TFs and JA-signaling repressors from the complex. Inositol pentakisphosphate (InsP5), a co-receptor for JA-Ile, stabilizes the association of COI1–JAZ complex [37]. Depending upon the nature of infecting pathogens, the JA signaling operates through either (i) MYC or (ii) ERF TFs. The regulation of JA signaling in the MYC2 branch involves the association of HAC1 with MYC2 and MED25 proteins and binding of various MYC TFs [38]. This activator complex then induces the transcription of the downstream JA-responsive gene (e.g., VSP2) (i). The regulation of JA signaling through the ERF branch is mediated by ethylene (ET), EIN3, and EIL1. EIN3 and EIL1 TFs induce the transcription of ERF1 and ORA59. ERF1 and ORA59 TFs, in turn, recruit MED25 to the GCC-box motif in the target gene promoters, leading to the activation of JA-responsive genes (e.g., PDF1.2) [39] (ii). Double-headed bars show mutual repression, while dotted arrows indicate the release of TFs from the repressor complex. L-arrows and L-bars indicate induction and repression of gene transcription, respectively. Crosses indicate the inability of proteins to bind to DNA sequences. COI1, CORONATINE INSENSITIVE 1 receptor; EIL1, ETHYLENE-INSENSITIVE 3-LIKE 1; EIN3, ETHYLENE-INSENSITIVE 3; ERF, ETHYLENE RESPONSE FACTOR; GTFs, general transcription factors; HAC1, HISTONE ACETYLTRANSFERASE OF THE CBP FAMILY 1; HDA, HISTONE DEACETYLASE; InsP5, inositol pentakisphosphate; JA-Ile, isoleucine conjugated jasmonate; JAT1, JASMONATE TRANSPORTER 1; JAZ, JASMONATE ZINC-FINGER EXPRESSED IN INFLORESCENCE MERISTEM (ZIM)-DOMAIN PROTEIN; MED25, a subunit of the MEDIATOR transcriptional co-activator complex; MYC, MYELOCYTOMATOSIS; NINJA, NOVEL INTERACTOR OF JAZ; ORA59, OCTADECANOID-RESPONSIVE ARABIDOPSIS AP2 (APETALA2)/ERF59; PDF1.2, PLANT DEFENSIN 1.2; RNA Pol II, RNA POLYMERASE II; TPL, TOPLESS; TPR, TPL-RELATED proteins; VSP2, VEGETATIVE STORAGE PROTEIN 2.
Ijms 21 07482 g002
Ijms 21 07482 g003 550
Figure 3. Negative regulation of the jasmonic acid (JA) signaling in Arabidopsis. (a) JAZs bind to transcription factors (TFs), such as JAM1 and JAM2, and compete with MYC2 for binding to the promoters of JA-responsive genes to repress their transcription [51]. (b) JAZ proteins (e.g., JAZ8) also interact with JASMONATE-ASSOCIATED VQ MOTIF GENE 1 (JAV1) and WRKY51 to form a “JJW” co-repressor complex. The JJW complex, in turn, binds to the promoter of JA-biosynthetic genes (e.g., AOS) and represses their expression, inhibiting JA biosynthesis [53]. (c) MYC2 is polyubiquitinated by PUB10 and is targeted for degradation, while it is stabilized by UBP12- and UBP13-mediated deubiquitination [54,55]. (d) The JA-signaling pathway is subjected to feedback regulation by a CUL3BPM E3 ligase system. The BPM proteins interact with MYC2, 3, and 4 and associate these proteins with the CUL3 BPM E3 ligase complex for ubiquitination and subsequent breakdown. This process leads to the transcription repression of the downstream JA-responsive genes [56]. Bars show repression, and L-bars indicate repression of gene transcription. Cross indicates the inability of proteins to bind to DNA sequences. AOS, ALLENE OXIDE SYNTHASE; BPM, ((BTB/POZ (BROAD COMPLEX, TRAMTRACK, BRIC-A-BRAC/POX VIRUS, AND ZINC FINGER DOMAIN) and MATH (MEPRIN AND TRAF HOMOLOGY DOMAIN)); CUL3, CULIN3; JAM, JA-ASSOCIATED MYC2-LIKE; JAV1, JASMONATE-ASSOCIATED VQ MOTIF GENE 1; JAZ, JASMONATE ZINC-FINGER EXPRESSED IN INFLORESCENCE MERISTEM (ZIM)-DOMAIN PROTEIN; JJW, JAV1, JAZ, WRKY51; MED25, a subunit of the MEDIATOR transcriptional co-activator complex; MYC, MYELOCYTOMATOSIS; PUB10, PLANT U-BOX PROTEIN 10; UBP, UBIQUITIN-SPECIFIC PROTEASE.
Figure 3. Negative regulation of the jasmonic acid (JA) signaling in Arabidopsis. (a) JAZs bind to transcription factors (TFs), such as JAM1 and JAM2, and compete with MYC2 for binding to the promoters of JA-responsive genes to repress their transcription [51]. (b) JAZ proteins (e.g., JAZ8) also interact with JASMONATE-ASSOCIATED VQ MOTIF GENE 1 (JAV1) and WRKY51 to form a “JJW” co-repressor complex. The JJW complex, in turn, binds to the promoter of JA-biosynthetic genes (e.g., AOS) and represses their expression, inhibiting JA biosynthesis [53]. (c) MYC2 is polyubiquitinated by PUB10 and is targeted for degradation, while it is stabilized by UBP12- and UBP13-mediated deubiquitination [54,55]. (d) The JA-signaling pathway is subjected to feedback regulation by a CUL3BPM E3 ligase system. The BPM proteins interact with MYC2, 3, and 4 and associate these proteins with the CUL3 BPM E3 ligase complex for ubiquitination and subsequent breakdown. This process leads to the transcription repression of the downstream JA-responsive genes [56]. Bars show repression, and L-bars indicate repression of gene transcription. Cross indicates the inability of proteins to bind to DNA sequences. AOS, ALLENE OXIDE SYNTHASE; BPM, ((BTB/POZ (BROAD COMPLEX, TRAMTRACK, BRIC-A-BRAC/POX VIRUS, AND ZINC FINGER DOMAIN) and MATH (MEPRIN AND TRAF HOMOLOGY DOMAIN)); CUL3, CULIN3; JAM, JA-ASSOCIATED MYC2-LIKE; JAV1, JASMONATE-ASSOCIATED VQ MOTIF GENE 1; JAZ, JASMONATE ZINC-FINGER EXPRESSED IN INFLORESCENCE MERISTEM (ZIM)-DOMAIN PROTEIN; JJW, JAV1, JAZ, WRKY51; MED25, a subunit of the MEDIATOR transcriptional co-activator complex; MYC, MYELOCYTOMATOSIS; PUB10, PLANT U-BOX PROTEIN 10; UBP, UBIQUITIN-SPECIFIC PROTEASE.
Ijms 21 07482 g003
Ijms 21 07482 g004 550
Figure 4. A summary of the Pseudomonas syringae factors manipulating jasmonic acid (JA) signaling. Different strains of P. syringae secrete virulence factors or effectors to gain control over the plant JA-signaling pathway. (a) P. syringae pv. tomato DC3000 secretes coronatine (COR), which is a mimic of the JA-Ile and perceived through the COI1–JAZ co-receptor complex. This COR–COI1–JAZ complex then directs JAZ proteins, such as JAZ2, JAZ5, and JAZ10, for degradation [69,86]. (b) P. syringae also releases type III effector HopX1, a cysteine protease that has been reported to interact with the ZIM domain of JAZ family members and degrade them [87]. (c) HopBB1 is another effector released by P. syringae and interacts with both TCP14 and JAZ3, the repressor components of JA signaling, and glues them together to facilitate their degradation in a COI1-dependent manner [85] (i). HopZ1a interacts with the C-terminal Jas domain of JAZ proteins and directs their degradation in a COI1-dependent manner [8] (ii). Together with RIN4, the AvrB effector activates the plasma membrane ATPase AHA1. This complex then causes an alteration in membrane potential and, through an unknown mechanism, increases the interaction between COI1 and JAZ, ultimately leading to degradation of the JAZ proteins [88] (iii). In all these cases, the degradation of JAZ proteins relieves the repression of transcription factors (TFs) and activation of JA signaling, as described in Figure 2, and leads to enhanced pathogen virulence. AHA1, Arabidopsis plasma membrane H+-ATPase; COI1, CORONATINE INSENSITIVE 1 receptor; COR, coronatine; JAZ, JASMONATE ZINC-FINGER EXPRESSED IN INFLORESCENCE MERISTEM (ZIM)-DOMAIN PROTEIN; InsP5, Inositol pentakisphosphate; NINJA, NOVEL INTERACTOR OF JAZ; RIN4, RESISTANCE TO P. syringae pv. maculicola 1 (RPM1)-INTERACTING 4; TCP14, TEOSINTE BRANCHED, CYCLOIDEA AND PROLIFERATING CELL FACTORS 14; TPL, TOPLESS; TPR, TPL-RELATED protein.
Figure 4. A summary of the Pseudomonas syringae factors manipulating jasmonic acid (JA) signaling. Different strains of P. syringae secrete virulence factors or effectors to gain control over the plant JA-signaling pathway. (a) P. syringae pv. tomato DC3000 secretes coronatine (COR), which is a mimic of the JA-Ile and perceived through the COI1–JAZ co-receptor complex. This COR–COI1–JAZ complex then directs JAZ proteins, such as JAZ2, JAZ5, and JAZ10, for degradation [69,86]. (b) P. syringae also releases type III effector HopX1, a cysteine protease that has been reported to interact with the ZIM domain of JAZ family members and degrade them [87]. (c) HopBB1 is another effector released by P. syringae and interacts with both TCP14 and JAZ3, the repressor components of JA signaling, and glues them together to facilitate their degradation in a COI1-dependent manner [85] (i). HopZ1a interacts with the C-terminal Jas domain of JAZ proteins and directs their degradation in a COI1-dependent manner [8] (ii). Together with RIN4, the AvrB effector activates the plasma membrane ATPase AHA1. This complex then causes an alteration in membrane potential and, through an unknown mechanism, increases the interaction between COI1 and JAZ, ultimately leading to degradation of the JAZ proteins [88] (iii). In all these cases, the degradation of JAZ proteins relieves the repression of transcription factors (TFs) and activation of JA signaling, as described in Figure 2, and leads to enhanced pathogen virulence. AHA1, Arabidopsis plasma membrane H+-ATPase; COI1, CORONATINE INSENSITIVE 1 receptor; COR, coronatine; JAZ, JASMONATE ZINC-FINGER EXPRESSED IN INFLORESCENCE MERISTEM (ZIM)-DOMAIN PROTEIN; InsP5, Inositol pentakisphosphate; NINJA, NOVEL INTERACTOR OF JAZ; RIN4, RESISTANCE TO P. syringae pv. maculicola 1 (RPM1)-INTERACTING 4; TCP14, TEOSINTE BRANCHED, CYCLOIDEA AND PROLIFERATING CELL FACTORS 14; TPL, TOPLESS; TPR, TPL-RELATED protein.
Ijms 21 07482 g004

Share and Cite

MDPI and ACS Style

Gupta, A.; Bhardwaj, M.; Tran, L.-S.P. Jasmonic Acid at the Crossroads of Plant Immunity and Pseudomonas syringae Virulence. Int. J. Mol. Sci. 2020, 21, 7482. https://doi.org/10.3390/ijms21207482

AMA Style

Gupta A, Bhardwaj M, Tran L-SP. Jasmonic Acid at the Crossroads of Plant Immunity and Pseudomonas syringae Virulence. International Journal of Molecular Sciences. 2020; 21(20):7482. https://doi.org/10.3390/ijms21207482

Chicago/Turabian Style

Gupta, Aarti, Mamta Bhardwaj, and Lam-Son Phan Tran. 2020. "Jasmonic Acid at the Crossroads of Plant Immunity and Pseudomonas syringae Virulence" International Journal of Molecular Sciences 21, no. 20: 7482. https://doi.org/10.3390/ijms21207482

APA Style

Gupta, A., Bhardwaj, M., & Tran, L. -S. P. (2020). Jasmonic Acid at the Crossroads of Plant Immunity and Pseudomonas syringae Virulence. International Journal of Molecular Sciences, 21(20), 7482. https://doi.org/10.3390/ijms21207482

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop