Antiphotoaging Effect of 3,5-Dicaffeoyl-epi-quinic Acid against UVA-Induced Skin Damage by Protecting Human Dermal Fibroblasts In Vitro
Abstract
:1. Introduction
2. Results
2.1. Cytotoxicity of DEQA Treatment and UVA Irradiation in HDFs
2.2. Effect of DEQA on the Release of MMP-1 and Collagen I in UVA-Irradiated HDFs
2.3. Effect of DEQA on MMPs and Type I Procollagen Expressions in UVA-Irradiated HDFs
2.4. Effect of DEQA on MAPK/AP-1 Signaling in UVA-Irradiated HDFs
2.5. Effect of DEQA on Collagen Production in UVA-Irradiated HDFs
2.6. Effect of DEQA on TGF-β/Smad Signaling in UVA-Irradiated HDFs
3. Discussion
4. Materials and Methods
4.1. Cell Culture and UVA Irradiation
4.2. Cytotoxicity Assay
4.3. Enzyme-Linked Immunosorbent Assay (ELISA)
4.4. RNA Extraction and Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) Analysis
4.5. Western Blot Analysis
4.6. Immunohistochemistry (IHC)
4.7. MAPK Activation Assay
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Oh, J.H.; Karadeniz, F.; Kong, C.-S.; Seo, Y. Antiphotoaging Effect of 3,5-Dicaffeoyl-epi-quinic Acid against UVA-Induced Skin Damage by Protecting Human Dermal Fibroblasts In Vitro. Int. J. Mol. Sci. 2020, 21, 7756. https://doi.org/10.3390/ijms21207756
Oh JH, Karadeniz F, Kong C-S, Seo Y. Antiphotoaging Effect of 3,5-Dicaffeoyl-epi-quinic Acid against UVA-Induced Skin Damage by Protecting Human Dermal Fibroblasts In Vitro. International Journal of Molecular Sciences. 2020; 21(20):7756. https://doi.org/10.3390/ijms21207756
Chicago/Turabian StyleOh, Jung Hwan, Fatih Karadeniz, Chang-Suk Kong, and Youngwan Seo. 2020. "Antiphotoaging Effect of 3,5-Dicaffeoyl-epi-quinic Acid against UVA-Induced Skin Damage by Protecting Human Dermal Fibroblasts In Vitro" International Journal of Molecular Sciences 21, no. 20: 7756. https://doi.org/10.3390/ijms21207756