DNA Double-Strand Breaks Are a Critical Regulator of Fear Memory Reconsolidation
Abstract
:1. Introduction
2. Results
2.1. Gene-Specific Increases in H2A.XpS139 Levels in the Dorsal Hippocampus Following Memory Retrieval
2.2. Gene-Specific H3K4me3 Levels Increase Simultaneously with H2A.XpS139 in the Dorsal Hippocampus Following Retrieval
2.3. Knockdown of TopIIβ in the Dorsal Hippocampus Abolishes Retrieval-Induced Increases in Gene-Specific H2A.XpS139 and H3K4me3
2.4. Knockdown of TopIIβ in the Dorsal Hippocampus Prior to Retrieval Impairs Memory Reconsolidation
3. Discussion
4. Materials and Methods
4.1. Animals (Subjects)
4.2. Surgery and siRNA Delivery
4.3. Cell Culture
4.4. cDNA Synthesis and Quantitative Real-Time PCR
4.5. Apparatus
4.6. Behavioral Procedures
4.7. Tissue Collection and Protein Extraction
4.8. Histone Extraction
4.9. Antibodies
4.10. Western Blotting
4.11. Chromatin Immunoprecipitation (ChIP)
4.12. Statistical Analyses
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Navabpour, S.; Rogers, J.; McFadden, T.; Jarome, T.J. DNA Double-Strand Breaks Are a Critical Regulator of Fear Memory Reconsolidation. Int. J. Mol. Sci. 2020, 21, 8995. https://doi.org/10.3390/ijms21238995
Navabpour S, Rogers J, McFadden T, Jarome TJ. DNA Double-Strand Breaks Are a Critical Regulator of Fear Memory Reconsolidation. International Journal of Molecular Sciences. 2020; 21(23):8995. https://doi.org/10.3390/ijms21238995
Chicago/Turabian StyleNavabpour, Shaghayegh, Jessie Rogers, Taylor McFadden, and Timothy J. Jarome. 2020. "DNA Double-Strand Breaks Are a Critical Regulator of Fear Memory Reconsolidation" International Journal of Molecular Sciences 21, no. 23: 8995. https://doi.org/10.3390/ijms21238995