TGF-β Induced CTGF Expression in Human Lung Epithelial Cells through ERK, ADAM17, RSK1, and C/EBPβ Pathways
Abstract
:1. Introduction
2. Results
2.1. TGF-β Induced CTGF Expression in Human Lung Epithelial Cells
2.2. Involvement of ADAM17 Activation in TGF-β Induced CTGF Expression in A549 Cells
2.3. Mediating Effects of ERK on TGF-β-Induced CTGF Expression and ADAM17 Phosphorylation
2.4. RSK1-Mediated TGF-β-Induced CTGF Expression
2.5. Role of C/EBPβ in TGF-β-Induced CTGF
2.6. Activation of C/EBPβ by TGF-β through ERK, ADAM17, and RSK1
2.7. TGF-β-Induced EMT Process in Human Lung Epithelial Cells
2.8. Involvement of ADAM17 and CTGF in TGF-β-Induced FN Expression
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Culture
4.3. siRNAs Transfection
4.4. Western Blotting
4.5. Chromatin Immunoprecipitation Assay
4.6. RNA Isolation and RT-PCR
4.7. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ADAM17 | A disintegrin and metalloproteinase 17 |
ANOVA | Analysis of variance |
C/EBPβ | CCAAT/enhancer-binding protein β |
ChIP | Chromatin immunoprecipitation |
CTGF | Connective tissue growth factor |
ECM | Extracellular matrix |
EMT | Epithelial–mesenchymal transition |
ERK | Extracellular signal-regulated kinase |
FN | Fibronectin |
IPF | Idiopathic pulmonary fibrosis |
RSK | Ribosomal S6 kinases 1 |
siRNA | Small interfering RNA |
TGF-β | Transforming growth factor-β |
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Ou, S.-C.; Bai, K.-J.; Cheng, W.-H.; Chen, J.-Y.; Lin, C.-H.; Wen, H.-C.; Chen, B.-C. TGF-β Induced CTGF Expression in Human Lung Epithelial Cells through ERK, ADAM17, RSK1, and C/EBPβ Pathways. Int. J. Mol. Sci. 2020, 21, 9084. https://doi.org/10.3390/ijms21239084
Ou S-C, Bai K-J, Cheng W-H, Chen J-Y, Lin C-H, Wen H-C, Chen B-C. TGF-β Induced CTGF Expression in Human Lung Epithelial Cells through ERK, ADAM17, RSK1, and C/EBPβ Pathways. International Journal of Molecular Sciences. 2020; 21(23):9084. https://doi.org/10.3390/ijms21239084
Chicago/Turabian StyleOu, Shu-Ching, Kuan-Jen Bai, Wun-Hao Cheng, Jing-Yun Chen, Chien-Huang Lin, Heng-Ching Wen, and Bing-Chang Chen. 2020. "TGF-β Induced CTGF Expression in Human Lung Epithelial Cells through ERK, ADAM17, RSK1, and C/EBPβ Pathways" International Journal of Molecular Sciences 21, no. 23: 9084. https://doi.org/10.3390/ijms21239084