Specificity of Interactions between Components of Two Zinc ABC Transporters in Paracoccus denitrificans
Abstract
:1. Introduction
2. Results
2.1. Zinc Transport in Hybrid znu and azt Transporter Systems
2.2. Flexible Loop Structures Are Not Essential for Zinc Transfer
2.3. Cluster A-I SBP Homology
2.4. The ZrgA-Like Proteins
3. Discussion
4. Materials and Methods
4.1. Strains and Media
4.2. Construction of P. denitrificans Deletion Mutant Strains
4.3. Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)
4.4. Cell Growth Determination
4.5. Colony Forming Units Assay
4.6. Sequence Alignments
4.7. Phylogenetic Analysis of ZrgA
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
SBP | Substrate binding proteins |
ABC | ATP binding cassette |
CFU | Colony Forming Units |
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Meléndez, A.B.; Valencia, D.; Yukl, E.T. Specificity of Interactions between Components of Two Zinc ABC Transporters in Paracoccus denitrificans. Int. J. Mol. Sci. 2020, 21, 9098. https://doi.org/10.3390/ijms21239098
Meléndez AB, Valencia D, Yukl ET. Specificity of Interactions between Components of Two Zinc ABC Transporters in Paracoccus denitrificans. International Journal of Molecular Sciences. 2020; 21(23):9098. https://doi.org/10.3390/ijms21239098
Chicago/Turabian StyleMeléndez, Ady Berenice, Daniel Valencia, and Erik Thomas Yukl. 2020. "Specificity of Interactions between Components of Two Zinc ABC Transporters in Paracoccus denitrificans" International Journal of Molecular Sciences 21, no. 23: 9098. https://doi.org/10.3390/ijms21239098