Oxytocin-Dependent Regulation of TRPs Expression in Trigeminal Ganglion Neurons Attenuates Orofacial Neuropathic Pain following Infraorbital Nerve Injury in Rats
Abstract
:1. Introduction
2. Results
2.1. Effect of Local OXT Administration on Post-IONI Orofacial Pain Hypersensitivity
2.2. OXT Receptor Expression in the TG and ION
2.3. Effect of OXT Administration on the TG Neuronal TRPV1 and TRPV4 Expression following IONI
2.4. The Inhibitory Effect of TRP Antagonism on IONI-Induced Orofacial Pain Hypersensitivity
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Infraorbital Nerve Injury
4.3. Assessment of Orofacial Nociception
4.3.1. Mechanical Sensitivity
4.3.2. Heat Sensitivity
4.4. Drug Administration
4.5. Immunohistochemistry
4.6. Western Blotting
4.7. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
Abbreviations
IONI | Infraorbital nerve injury |
OXT | Oxytocin |
Ato | Atosiban |
TG | Trigeminal ganglion |
MHWT | Mechanical head-withdrawal threshold |
HHRT | Heat head-withdrawal threshold |
FG | FluoroGold |
TRPV | Transient receptor potential vanilloid |
PGP | Protein gene product |
IB4 | Isolectin B4 |
CGRP | Calcitonin gene-related peptide |
ASICs | Acid-sensing ion channels |
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Ando, M.; Hayashi, Y.; Hitomi, S.; Shibuta, I.; Furukawa, A.; Oto, T.; Inada, T.; Matsui, T.; Fukaya, C.; Noma, N.; et al. Oxytocin-Dependent Regulation of TRPs Expression in Trigeminal Ganglion Neurons Attenuates Orofacial Neuropathic Pain following Infraorbital Nerve Injury in Rats. Int. J. Mol. Sci. 2020, 21, 9173. https://doi.org/10.3390/ijms21239173
Ando M, Hayashi Y, Hitomi S, Shibuta I, Furukawa A, Oto T, Inada T, Matsui T, Fukaya C, Noma N, et al. Oxytocin-Dependent Regulation of TRPs Expression in Trigeminal Ganglion Neurons Attenuates Orofacial Neuropathic Pain following Infraorbital Nerve Injury in Rats. International Journal of Molecular Sciences. 2020; 21(23):9173. https://doi.org/10.3390/ijms21239173
Chicago/Turabian StyleAndo, Masatoshi, Yoshinori Hayashi, Suzuro Hitomi, Ikuko Shibuta, Akihiko Furukawa, Tatsuki Oto, Takanobu Inada, Tomoyuki Matsui, Chikashi Fukaya, Noboru Noma, and et al. 2020. "Oxytocin-Dependent Regulation of TRPs Expression in Trigeminal Ganglion Neurons Attenuates Orofacial Neuropathic Pain following Infraorbital Nerve Injury in Rats" International Journal of Molecular Sciences 21, no. 23: 9173. https://doi.org/10.3390/ijms21239173