Serenoa repens and Urtica dioica Fixed Combination: In-Vitro Validation of a Therapy for Benign Prostatic Hyperplasia (BPH)
Abstract
:1. Introduction
2. Results
2.1. Activity of SR and SR/UD in Cell Proliferation
2.2. Antioxidant Effect of SR and SR/UD
2.3. Anti-Inflammatory Effect of SR and SR/UD on BPH-1 Cells
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Cell Cultures
5.2. SR Composition
5.3. SR/UD Composition
- lipophilic extract from fruits of S. repens [160 mg WS® 1473; drug–extract ratio 10.0–14.3:1; extraction solvent: 90% ethanol (m/m)]
- aqueous ethanolic extract from roots of U. dioica [120mg WS® 1031, drug–extract ratio 7.6–12.5:1; extraction solvent: 60% ethanol (m/m)].
5.4. Cells Viability Assay
5.5. ROS Assay
5.6. Nuclear Factor-kappa B Translocation Assay
5.7. RNA Expression/Quantitative Real-Time PCR
5.8. IL-6 and IL-8 ELISA
5.9. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
Appendix A
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Saponaro, M.; Giacomini, I.; Morandin, G.; Cocetta, V.; Ragazzi, E.; Orso, G.; Carnevali, I.; Berretta, M.; Mancini, M.; Pagano, F.; et al. Serenoa repens and Urtica dioica Fixed Combination: In-Vitro Validation of a Therapy for Benign Prostatic Hyperplasia (BPH). Int. J. Mol. Sci. 2020, 21, 9178. https://doi.org/10.3390/ijms21239178
Saponaro M, Giacomini I, Morandin G, Cocetta V, Ragazzi E, Orso G, Carnevali I, Berretta M, Mancini M, Pagano F, et al. Serenoa repens and Urtica dioica Fixed Combination: In-Vitro Validation of a Therapy for Benign Prostatic Hyperplasia (BPH). International Journal of Molecular Sciences. 2020; 21(23):9178. https://doi.org/10.3390/ijms21239178
Chicago/Turabian StyleSaponaro, Miriam, Isabella Giacomini, Giulia Morandin, Veronica Cocetta, Eugenio Ragazzi, Genny Orso, Ilaria Carnevali, Massimiliano Berretta, Mariangela Mancini, Francesco Pagano, and et al. 2020. "Serenoa repens and Urtica dioica Fixed Combination: In-Vitro Validation of a Therapy for Benign Prostatic Hyperplasia (BPH)" International Journal of Molecular Sciences 21, no. 23: 9178. https://doi.org/10.3390/ijms21239178