The 3D Pattern of the Rainbow Trout (Oncorhynchus mykiss) Enterocytes and Intestinal Stem Cells
Abstract
:1. Introduction
2. Results
2.1. Distribution of Functional Brush Borders Proteins along the Mid-Intestine
2.2. The Intestinal Stem-Cell Niche Morphological and Molecular Architecture
2.2.1. Lgr5
2.2.2. Hopx
2.2.3. Sox9
2.2.4. Notch1
2.2.5. Dll1
2.2.6. Wnt3a
3. Discussion
4. Materials and Methods
4.1. Sample Collection and Processing
4.2. Histology and Immunohistochemistry
4.3. Target Probe Design and Fluoresce in Situ Hybridization (FISH)
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
RT | Rainbow trout |
PEPT1 | Peptide transporter 1 |
SGLT1 | Sodium–glucose/galactose transporter 1 |
FABP2 | Fatty-acid-binding protein 2 |
ISCs | Intestinal stem cells |
LGR5 | Leucine-rich-repeat-containing G-protein-coupled receptor 5 |
HOPX | Homeodomain-only protein |
SOX9 | SRY-box 9 |
NOTCH1 | Notch receptor 1 |
DLL1 | Delta-like protein 1 |
WNT3A | Wnt family member 3A |
CBCs | Crypt base columnar cells |
GI | Gastrointestinal |
HRP | Horseradish peroxidase |
DAB | 3,3′-Diaminobenzidine |
DAPIFISH | 4′,6-diamidino-2-phenylindoleFluorescence in situ hybridization |
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mRNA Target | Channel | Cat.N. |
---|---|---|
lgr5 | Channel 1 | 847731 |
hopx | Channel 2 | 847761-C2 |
wnt3a | Channel 2 | 847771-C2 |
sox9 | Channel 3 | 847751-C3 |
notch | Channel 3 | 847741-C3 |
dll1 | Channel 3 | 853451-C3 |
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Verdile, N.; Pasquariello, R.; Brevini, T.A.L.; Gandolfi, F. The 3D Pattern of the Rainbow Trout (Oncorhynchus mykiss) Enterocytes and Intestinal Stem Cells. Int. J. Mol. Sci. 2020, 21, 9192. https://doi.org/10.3390/ijms21239192
Verdile N, Pasquariello R, Brevini TAL, Gandolfi F. The 3D Pattern of the Rainbow Trout (Oncorhynchus mykiss) Enterocytes and Intestinal Stem Cells. International Journal of Molecular Sciences. 2020; 21(23):9192. https://doi.org/10.3390/ijms21239192
Chicago/Turabian StyleVerdile, Nicole, Rolando Pasquariello, Tiziana A. L. Brevini, and Fulvio Gandolfi. 2020. "The 3D Pattern of the Rainbow Trout (Oncorhynchus mykiss) Enterocytes and Intestinal Stem Cells" International Journal of Molecular Sciences 21, no. 23: 9192. https://doi.org/10.3390/ijms21239192