HSP70-Homolog DnaK of Pseudomonas aeruginosa Increases the Production of IL-27 through Expression of EBI3 via TLR4-Dependent NF-κB and TLR4-Independent Akt Signaling
Abstract
:1. Introduction
2. Results
2.1. P. aeruginosa-Mediated Induction of EBI3 Expression
2.2. P. aeruginosa DnaK Induces Expression of EBI3
2.3. DnaK-Induced EBI3 Is Involved in the Formation of IL-27
2.4. IL-27 Induced by DnaK Promotes Expression of IL10
2.5. DnaK-Induced Expression of EBI3 Is under the Control of the NF-κB and Akt Signaling Pathways
2.6. DnaK-Mediated Induction of EBI3 Expression Is Partly under the Control of TLR4
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains and Culture Conditions
4.2. Cell Culture
4.3. Construction and Purification of rDnaK
4.4. Quantitative Real-Time PCR (qRT-PCR)
4.5. Immunoblotting Analysis
4.6. Enzyme-Linked Immunosorbent Assay (ELISA)
4.7. Transfection of siRNA
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Jeon, J.; Lee, Y.; Yu, H.; Ha, U.-H. HSP70-Homolog DnaK of Pseudomonas aeruginosa Increases the Production of IL-27 through Expression of EBI3 via TLR4-Dependent NF-κB and TLR4-Independent Akt Signaling. Int. J. Mol. Sci. 2020, 21, 9194. https://doi.org/10.3390/ijms21239194
Jeon J, Lee Y, Yu H, Ha U-H. HSP70-Homolog DnaK of Pseudomonas aeruginosa Increases the Production of IL-27 through Expression of EBI3 via TLR4-Dependent NF-κB and TLR4-Independent Akt Signaling. International Journal of Molecular Sciences. 2020; 21(23):9194. https://doi.org/10.3390/ijms21239194
Chicago/Turabian StyleJeon, Jisu, Yeji Lee, Hyeonseung Yu, and Un-Hwan Ha. 2020. "HSP70-Homolog DnaK of Pseudomonas aeruginosa Increases the Production of IL-27 through Expression of EBI3 via TLR4-Dependent NF-κB and TLR4-Independent Akt Signaling" International Journal of Molecular Sciences 21, no. 23: 9194. https://doi.org/10.3390/ijms21239194