The Topographical Optimization of 3D Microgroove Pattern Intervals for Ligamentous Cell Orientations: In Vitro
Abstract
:1. Introduction
2. Results
2.1. Morphological Analyses of PDL-Guiding Architectures
2.2. Quantification of Collective Cell Orientations for Microgroove Optimizations
2.3. Quantification Assessments of Nuclear Deformation
3. Discussion
4. Materials and Methods
4.1. PDL-Guiding Architecture Design and Biopolymeric Scaffold Fabrication
4.2. Surface Characterizations of Microgroove Patterns on PDL-Guiding Architectures
4.3. Cell Orientation and Nuclear Deformation Analyses in In Vitro
4.4. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
PDL | Periodontal ligament |
3D | Three dimensional |
μG-25 | Microgroove pattern interval with 25.40 μm |
μG-19 | Microgroove pattern interval with 19.05 μm |
μG-12 | Microgroove pattern interval with 12.70 μm |
μG-6 | Microgroove pattern interval with 6.35 μm |
PCL | poly-ε-caprolactone |
SEM | Scanning electron microscope |
DAPI | 4′,6-diamidino-2-phenylindole |
NAR | Nuclear aspect ratio |
NSI | Nuclear shape index |
CAD | Computer-aided design |
α-MEM | Minimum essential medium alpha |
FBS | Fetal bovine serum |
ANOVA | Analysis of variance |
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Kim, M.G.; Park, C.H. The Topographical Optimization of 3D Microgroove Pattern Intervals for Ligamentous Cell Orientations: In Vitro. Int. J. Mol. Sci. 2020, 21, 9358. https://doi.org/10.3390/ijms21249358
Kim MG, Park CH. The Topographical Optimization of 3D Microgroove Pattern Intervals for Ligamentous Cell Orientations: In Vitro. International Journal of Molecular Sciences. 2020; 21(24):9358. https://doi.org/10.3390/ijms21249358
Chicago/Turabian StyleKim, Min Guk, and Chan Ho Park. 2020. "The Topographical Optimization of 3D Microgroove Pattern Intervals for Ligamentous Cell Orientations: In Vitro" International Journal of Molecular Sciences 21, no. 24: 9358. https://doi.org/10.3390/ijms21249358