Effects of Liposome and Cardiolipin on Folding and Function of Mitochondrial Erv1
Abstract
:1. Introduction
2. Results
2.1. Preparation and Characterization of Liposomes
2.2. Effects of Liposomes on the Oxidase Activity of Erv1
2.3. Effects of Liposomes on Cytochrome c Reductase Activity of Erv1
3. Discussion
4. Materials and Methods
4.1. Protein Expression and Purification
4.2. Liposome Preparation
4.3. UV-Visible Spectroscopy
4.4. Oxygen Consumption Assays
4.5. Cytochrome c Reduction Assay
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ALR | Augmenter of liver regeneration |
DTT | Dithiothreitol |
DOPC | 1,2-dioleoyl-sn-glycero-3-phosphocholine |
DOPCE | 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine |
Erv | Essential for respiration and viability |
FAD | Flavin adenine dinucleotide |
IMS | Intermembrane space |
IM | Inner membrane |
MIA | Mitochondrial import and assembly |
OM | Outer membrane |
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Substrate | Liposomes | kcat (s−1) | Km (µM) | kcat/Km (M−1 s−1) |
---|---|---|---|---|
Oxygen | Control (No liposomes) | 1.0 ± 0.1 | 50 ± 10 | 2.0 × 104 (100%) |
CL0 | 0.82 ± 0.02 | 60.0 ± 6 | 1.4 × 104 (70%) | |
CL5 | 0.74 ± 0.01 | 58.8 ± 6 | 1.2 × 104 (60%) | |
CL15 | 0.76 ± 0.02 | 53.2 ± 5 | 1.4 × 104 (70%) | |
Cytochrome c | Control (No liposomes) | 0.82 ± 0.07 | 23 ± 4 | 3.6 × 104 (100%) |
CL0 | 0.75 ± 0.03 | 52 ± 4 | 1.4 × 104 (39%) | |
CL5 | 0.61 ± 0.04 | 74 ± 3 | 8.2 × 103 (23%) | |
CL15 | 0.48 ± 0.02 | 105 ± 7 | 4.6 × 103 (13%) |
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Tang, X.; Harris, L.K.; Lu, H. Effects of Liposome and Cardiolipin on Folding and Function of Mitochondrial Erv1. Int. J. Mol. Sci. 2020, 21, 9402. https://doi.org/10.3390/ijms21249402
Tang X, Harris LK, Lu H. Effects of Liposome and Cardiolipin on Folding and Function of Mitochondrial Erv1. International Journal of Molecular Sciences. 2020; 21(24):9402. https://doi.org/10.3390/ijms21249402
Chicago/Turabian StyleTang, Xiaofan, Lynda K Harris, and Hui Lu. 2020. "Effects of Liposome and Cardiolipin on Folding and Function of Mitochondrial Erv1" International Journal of Molecular Sciences 21, no. 24: 9402. https://doi.org/10.3390/ijms21249402