Suppression of Pax3–MITF-M Axis Protects from UVB-Induced Skin Pigmentation by Tetrahydroquinoline Carboxamide
Abstract
:1. Introduction
2. Results
2.1. Topical Treatment with THQC Mitigated UVB-Induced Skin Pigmentation
2.2. THQC Inhibited Melanin Production in cAMP-Elevated B16-F0 Culture
2.3. THQC Downregulated α-MSH-Induced Tyro Expression
2.4. THQC Inhibited α-MSH-Induced Promoter Activity of the MITF-M Gene
2.5. THQC Decreased Pax3 Level for Inhibiting Facultative Melanin Pigmentation
3. Discussion
4. Materials and Methods
4.1. Chemicals and Cell Culture
4.2. UVB-Induced Skin Pigmentation
4.3. Western Blot Analysis
4.4. RT-PCR Analysis
4.5. Melanin Quantification
4.6. Cell Viability Assay
4.7. Dopa Oxidation Assay
4.8. Luciferase Reporter Assay
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
CREB | cAMP responsive element-binding protein |
db-cAMP | dibutyryl cyclic adenosine monophosphate |
GAPDH | glyceraldehyde 3-phosphate dehydrogenase |
MITF-M | microphthalmia-associated transcription factor isoform M |
MSH | melanocyte-stimulating hormone |
Pax3 | paired box gene 3 |
SOX10 | SRY-related HMG-box 10 |
THQC | tetrahydroquinoline carboxamide |
Tyro | tyrosinase |
UVB | ultraviolet B |
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Choi, Y.-P.; Kim, G.H.; Kim, S.-H.; Maeng, J.; Lee, H.; Han, S.-B.; Kim, K.H.; Kim, Y. Suppression of Pax3–MITF-M Axis Protects from UVB-Induced Skin Pigmentation by Tetrahydroquinoline Carboxamide. Int. J. Mol. Sci. 2020, 21, 9631. https://doi.org/10.3390/ijms21249631
Choi Y-P, Kim GH, Kim S-H, Maeng J, Lee H, Han S-B, Kim KH, Kim Y. Suppression of Pax3–MITF-M Axis Protects from UVB-Induced Skin Pigmentation by Tetrahydroquinoline Carboxamide. International Journal of Molecular Sciences. 2020; 21(24):9631. https://doi.org/10.3390/ijms21249631
Chicago/Turabian StyleChoi, Yong-Pyo, Ga Hyun Kim, Song-Hee Kim, Jongseo Maeng, Heesoon Lee, Sang-Bae Han, Ki Ho Kim, and Youngsoo Kim. 2020. "Suppression of Pax3–MITF-M Axis Protects from UVB-Induced Skin Pigmentation by Tetrahydroquinoline Carboxamide" International Journal of Molecular Sciences 21, no. 24: 9631. https://doi.org/10.3390/ijms21249631