Functional Analysis Reveals the Regulatory Role of PpTST1 Encoding Tonoplast Sugar Transporter in Sugar Accumulation of Peach Fruit
Abstract
:1. Introduction
2. Results
2.1. The Expression Profile of PpTST1 Showed a Consistency with the Accumulation Pattern of Soluble Sugars in Peach Fruits
2.2. The Impact of PpTST1 on Fruit Sugar Accumulation
2.3. Assay for Subcelluar Localization of PpTST1 in Tobacco Leaves
2.4. Transient Silencing of PpTST1 Inhibits Sugar Accumulation in Peach Fruit
2.5. Multiple Cis-Elements Are Present in the Promoter Region of PpTST1
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. DNA Extraction and dCAPS Marker Development
4.3. RNA Isolation and RT-qPCR Analysis
4.4. Measurement of Sugar Content and Composition in Peach Fruit
4.5. Subcellular Localization Analysis of PpTST1 in Tobacco Leaves
4.6. Functional Analysis of PpTST1 Using the VIGS System in Peach Fruit
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Peng, Q.; Wang, L.; Ogutu, C.; Liu, J.; Liu, L.; Mollah, M.D.A.; Han, Y. Functional Analysis Reveals the Regulatory Role of PpTST1 Encoding Tonoplast Sugar Transporter in Sugar Accumulation of Peach Fruit. Int. J. Mol. Sci. 2020, 21, 1112. https://doi.org/10.3390/ijms21031112
Peng Q, Wang L, Ogutu C, Liu J, Liu L, Mollah MDA, Han Y. Functional Analysis Reveals the Regulatory Role of PpTST1 Encoding Tonoplast Sugar Transporter in Sugar Accumulation of Peach Fruit. International Journal of Molecular Sciences. 2020; 21(3):1112. https://doi.org/10.3390/ijms21031112
Chicago/Turabian StylePeng, Qian, Lu Wang, Collins Ogutu, Jingjing Liu, Li Liu, Md. Dulal Ali Mollah, and Yuepeng Han. 2020. "Functional Analysis Reveals the Regulatory Role of PpTST1 Encoding Tonoplast Sugar Transporter in Sugar Accumulation of Peach Fruit" International Journal of Molecular Sciences 21, no. 3: 1112. https://doi.org/10.3390/ijms21031112