Transposon Insertion Mutagenesis in Mice for Modeling Human Cancers: Critical Insights Gained and New Opportunities

Beckmann, Pauline J.; Largaespada, David A.

doi:10.3390/ijms21031172

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Transposon Insertion Mutagenesis in Mice for Modeling Human Cancers: Critical Insights Gained and New Opportunities

by

Pauline J. Beckmann

¹

and

David A. Largaespada

^1,2,3,4,*

¹

Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USA

²

Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA

³

Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA

⁴

Center for Genome Engineering, University of Minnesota, Minneapolis, MN 55455, USA

^*

Author to whom correspondence should be addressed.

Int. J. Mol. Sci. 2020, 21(3), 1172; https://doi.org/10.3390/ijms21031172

Submission received: 3 January 2020 / Revised: 30 January 2020 / Accepted: 3 February 2020 / Published: 10 February 2020

(This article belongs to the Special Issue Genetically Engineered Mice to Study Cancer)

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Abstract

Transposon mutagenesis has been used to model many types of human cancer in mice, leading to the discovery of novel cancer genes and insights into the mechanism of tumorigenesis. For this review, we identified over twenty types of human cancer that have been modeled in the mouse using Sleeping Beauty and piggyBac transposon insertion mutagenesis. We examine several specific biological insights that have been gained and describe opportunities for continued research. Specifically, we review studies with a focus on understanding metastasis, therapy resistance, and tumor cell of origin. Additionally, we propose further uses of transposon-based models to identify rarely mutated driver genes across many cancers, understand additional mechanisms of drug resistance and metastasis, and define personalized therapies for cancer patients with obesity as a comorbidity.

Keywords: animal modeling; cancer; transposon screen

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MDPI and ACS Style

Beckmann, P.J.; Largaespada, D.A. Transposon Insertion Mutagenesis in Mice for Modeling Human Cancers: Critical Insights Gained and New Opportunities. Int. J. Mol. Sci. 2020, 21, 1172. https://doi.org/10.3390/ijms21031172

AMA Style

Beckmann PJ, Largaespada DA. Transposon Insertion Mutagenesis in Mice for Modeling Human Cancers: Critical Insights Gained and New Opportunities. International Journal of Molecular Sciences. 2020; 21(3):1172. https://doi.org/10.3390/ijms21031172

Chicago/Turabian Style

Beckmann, Pauline J., and David A. Largaespada. 2020. "Transposon Insertion Mutagenesis in Mice for Modeling Human Cancers: Critical Insights Gained and New Opportunities" International Journal of Molecular Sciences 21, no. 3: 1172. https://doi.org/10.3390/ijms21031172

APA Style

Beckmann, P. J., & Largaespada, D. A. (2020). Transposon Insertion Mutagenesis in Mice for Modeling Human Cancers: Critical Insights Gained and New Opportunities. International Journal of Molecular Sciences, 21(3), 1172. https://doi.org/10.3390/ijms21031172

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Transposon Insertion Mutagenesis in Mice for Modeling Human Cancers: Critical Insights Gained and New Opportunities

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