Neonatal HDL Counteracts Placental Vascular Inflammation via S1P–S1PR1 Axis
Abstract
:1. Introduction
2. Results
nHDL-S1P Complex Attenuates Inflammation and Dysfunction of the Feto-Placental Endothelium
3. Discussion
4. Materials and Methods
4.1. Study Population
4.2. Isolation of Cord Blood-Derived Neonatal HDL and S1P Quantification
4.3. Isolation and Treatment of Primary Fetal Placental Arterial Endothelial Cells (fPAEC)
4.4. Quantitative Real-Time PCR (qPCR) and PrimePCR of fPAEC
4.5. Western Blot
4.6. FACS of fPAECs
4.7. Reactive Oxygen Species (ROS) Assay
4.8. Shotgun Proteomics by LC-MS/MS
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
Abbreviations
ApoM | Apolipoprotein M |
GPCR | G protein-coupled receptors |
S1PR | Sphingosine-1-phosphate receptor |
HSA | Human serum albumin |
TNFα | Tumor necrosis factor alpha |
AngI | Angiotensin II |
fPAEC | Human primary fetal placental endothelial cell |
ROS | Reactive oxygen species |
Nox1 | NADPH oxidase 1 |
ICAM | Intracellular adhesion molecule |
VCAM | Vascular cell adhesion molecule |
E-sele | Endothelial-leukocyte adhesion molecule |
IL-8 | Interleukin 8 |
MCP-1 | Monocyte chemoattractant protein 1 |
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Del Gaudio, I.; Hendrix, S.; Christoffersen, C.; Wadsack, C. Neonatal HDL Counteracts Placental Vascular Inflammation via S1P–S1PR1 Axis. Int. J. Mol. Sci. 2020, 21, 789. https://doi.org/10.3390/ijms21030789
Del Gaudio I, Hendrix S, Christoffersen C, Wadsack C. Neonatal HDL Counteracts Placental Vascular Inflammation via S1P–S1PR1 Axis. International Journal of Molecular Sciences. 2020; 21(3):789. https://doi.org/10.3390/ijms21030789
Chicago/Turabian StyleDel Gaudio, Ilaria, Sebastian Hendrix, Christina Christoffersen, and Christian Wadsack. 2020. "Neonatal HDL Counteracts Placental Vascular Inflammation via S1P–S1PR1 Axis" International Journal of Molecular Sciences 21, no. 3: 789. https://doi.org/10.3390/ijms21030789