The Rho/Rac Guanine Nucleotide Exchange Factor Vav1 Regulates Hif-1α and Glut-1 Expression and Glucose Uptake in the Brain
Abstract
:1. Introduction
2. Results
2.1. Vav1 Regulates HIF-1α and GLUT-1 in Endothelial Cells
2.2. Vav1 Regulates Glucose Uptake in Endothelial Cells
2.3. Vav1 Deficiency Delays Glucose Uptake In Vivo
2.4. Vav1 Deficiency Downregulates Glucose Distribution in the Brain
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Transfection and Lentiviral Transduction
4.3. Glucose Uptake Assay
4.4. Western Blot
4.5. RT-qPCR
4.6. Experimental Animals
4.7. Fear Conditioning
4.8. PET/CT Scanning
4.9. Histology
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Hong, J.; Kim, Y.; Yanpallewar, S.; Lin, P.C. The Rho/Rac Guanine Nucleotide Exchange Factor Vav1 Regulates Hif-1α and Glut-1 Expression and Glucose Uptake in the Brain. Int. J. Mol. Sci. 2020, 21, 1341. https://doi.org/10.3390/ijms21041341
Hong J, Kim Y, Yanpallewar S, Lin PC. The Rho/Rac Guanine Nucleotide Exchange Factor Vav1 Regulates Hif-1α and Glut-1 Expression and Glucose Uptake in the Brain. International Journal of Molecular Sciences. 2020; 21(4):1341. https://doi.org/10.3390/ijms21041341
Chicago/Turabian StyleHong, Jaewoo, Yurim Kim, Sudhirkumar Yanpallewar, and P. Charles Lin. 2020. "The Rho/Rac Guanine Nucleotide Exchange Factor Vav1 Regulates Hif-1α and Glut-1 Expression and Glucose Uptake in the Brain" International Journal of Molecular Sciences 21, no. 4: 1341. https://doi.org/10.3390/ijms21041341