Upregulation of Thr/Tyr kinase Increases the Cancer Progression by Neurotensin and Dihydropyrimidinase-Like 3 in Lung Cancer
Abstract
:1. Introduction
2. Results
2.1. Elevated TTK Expression Conferred Poor Prognosis in Lung Cancer Patients
2.2. TTK Decreased Cancer Growth by Interfering Cell Cycle Progression in Lung Cancer
2.3. TTK Regulated Metastatic Behaviors and EMT in Lung Cancer
2.4. NTS and DYPSL3 Act as Downstream Regulators of TTK
2.5. NTS Is Involved in TTK- Related Cell Proliferation Regulation
2.6. DPYSL3 Contributes the Enhancement of Cell Migration and EMT
2.7. Inhibition of TTK Suppressed Cancer Growth and Lung Metastasis In Vivo
3. Discussion
4. Materials and Methods
4.1. Cell Lines
4.2. Bioinformatics
4.3. NGS and Microarray
4.4. RNA-Sequencing and Quantitative Real-Time Polymerase Chain Reaction (Qrt-PCR)
4.5. Immunoblot and NTS Determination
4.6. TTK and DPYSL3 Knockdown
4.7. Cell Proliferation, Colony Formation and 5-Bromo-2-Deoxyuridine (Brdu) Incorporation
4.8. Cell Migration and Invasion
4.9. Cell Cycle Analysis
4.10. Animal Models
4.11. Immunohistochemistry (IHC)
4.12. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
DPYSL3 | dihydropyrimidinase-like 3 |
EMT | epithelial–mesenchymal transition |
Mps1 | monopolar spindle 1 |
NGS | next-generation sequencing |
NTS | neurotensin |
SAC | spindle assembly checkpoint |
TTK | Thr/Tyr kinase |
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Tsai, Y.-M.; Wu, K.-L.; Chang, Y.-Y.; Hung, J.-Y.; Chang, W.-A.; Chang, C.-Y.; Jian, S.-F.; Tsai, P.-H.; Huang, Y.-C.; Chong, I.-W.; et al. Upregulation of Thr/Tyr kinase Increases the Cancer Progression by Neurotensin and Dihydropyrimidinase-Like 3 in Lung Cancer. Int. J. Mol. Sci. 2020, 21, 1640. https://doi.org/10.3390/ijms21051640
Tsai Y-M, Wu K-L, Chang Y-Y, Hung J-Y, Chang W-A, Chang C-Y, Jian S-F, Tsai P-H, Huang Y-C, Chong I-W, et al. Upregulation of Thr/Tyr kinase Increases the Cancer Progression by Neurotensin and Dihydropyrimidinase-Like 3 in Lung Cancer. International Journal of Molecular Sciences. 2020; 21(5):1640. https://doi.org/10.3390/ijms21051640
Chicago/Turabian StyleTsai, Ying-Ming, Kuan-Li Wu, Yung-Yun Chang, Jen-Yu Hung, Wei-An Chang, Chao-Yuan Chang, Shu-Fang Jian, Pei-Hsun Tsai, Yung-Chi Huang, Inn-Wen Chong, and et al. 2020. "Upregulation of Thr/Tyr kinase Increases the Cancer Progression by Neurotensin and Dihydropyrimidinase-Like 3 in Lung Cancer" International Journal of Molecular Sciences 21, no. 5: 1640. https://doi.org/10.3390/ijms21051640