Lipofundin MCT/LCT Inhibits Levcromakalim-Induced Vasodilation by Inhibiting Endothelial Nitric Oxide Release
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Preparation of Isolated Rat Aorta and Isometric Tension Measurement
4.2. Experimental Protocol
4.3. Cell Culture
4.4. Measuring Membrane Potential
4.5. Western Blot
4.6. cGMP Measurement
4.7. Materials
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
KATP channel | ATP-sensitive potassium channel |
VSMC | Vascular smooth muscle cell |
NO | Nitric oxide |
PKC | Protein kinase C |
HUVEC | Human umbilical vein endothelial cell |
cGMP | Cyclic guanosine monophosphate |
L-NAME | NW-nitro-L-arginine methyl ester |
References
- Ok, S.H.; Hong, J.M.; Lee, S.H.; Sohn, J.T. Lipid emulsion for treating local anesthetic systemic toxicity. Int. J. Med. Sci. 2018, 15, 713–722. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Wanten, G.J.; Calder, P.C. Immune modulation by parenteral lipid emulsions. Am. J. Clin. Nutr. 2007, 85, 1171–1184. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Fettiplace, M.R.; Weinberg, G. The mechanisms underlying lipid resuscitation therapy. Reg. Anesth. Pain. Med. 2018, 43, 138–149. [Google Scholar] [CrossRef]
- Armstead, W.M. Role of ATP-sensitive K+ channels in cGMP-mediated pial artery vasodilation. Am. J. Physiol. 1996, 270, H423–H426. [Google Scholar] [CrossRef] [PubMed]
- Murphy, M.E.; Brayden, J.E. Nitric oxide hyperpolarizes rabbit mesenteric arteries via ATP-sensitive potassium channels. J. Physiol. 1995, 486, 47–58. [Google Scholar] [CrossRef]
- Kinoshita, H.; Iwahashi, S.; Kakutani, T.; Mizumoto, K.; Iranami, H.; Hatano, Y. The role of endothelium-derived nitric oxide in relaxations to levcromakalim in the rat aorta. Jpn. J. Pharmacol. 1999, 81, 362–366. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Flagg, T.P.; Enkvetchakul, D.; Koster, J.C.; Nichols, C.G. Muscle KATP channels: Recent insights to energy sensing and myoprotection. Physiol. Rev. 2010, 90, 799–829. [Google Scholar] [CrossRef] [Green Version]
- Umpierrez, G.E.; Smiley, D.; Robalino, G.; Peng, L.; Kitabchi, A.E.; Khan, B.; Le, A.; Quyyumi, A.; Brown, V.; Phillips, L.S. Intravenous intralipid-induced blood pressure elevation and endothelial dysfunction in obese African-Americans with type 2 diabetes. J. Clin. Endocrinol. Metab. 2009, 94, 609–614. [Google Scholar] [CrossRef] [Green Version]
- Gosmanov, A.R.; Smiley, D.D.; Peng, L.; Siquiera, J.; Robalino, G.; Newton, C.; Umpierrez, G.E. Vascular effects of intravenous intralipid and dextrose infusions in obese subjects. Metabolism 2012, 61, 1370–1376. [Google Scholar] [CrossRef] [Green Version]
- Stojiljkovic, M.P.; Zhang, D.; Lopes, H.F.; Lee, C.G.; Goodfriend, T.L.; Egan, B.M. Hemodynamic effects of lipids in humans. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2001, 280, R1674–R1679. [Google Scholar] [CrossRef]
- Ok, S.H.; Lee, S.H.; Yu, J.; Park, J.; Shin, I.W.; Lee, Y.; Cho, H.; Choi, M.J.; Baik, J.; Hong, J.M.; et al. Lipid emulsion attenuates acetylcholine-induced relaxation in isolated rat aorta. Biomed. Res. Int. 2015, 2015, 871545. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Brayden, J.E. Functional roles of KATP channels in vascular smooth muscle. Clin. Exp. Pharmacol. Physiol. 2002, 29, 312–316. [Google Scholar] [CrossRef] [PubMed]
- Neal, J.M.; Barrington, M.J.; Fettiplace, M.R.; Gitman, M.; Memtsoudis, S.G.; Mörwald, E.E.; Rubin, D.S.; Weinberg, G. The third american society of regional anesthesia and pain medicine practice advisory on local anesthetic systemic toxicity: Executive summary 2017. Reg. Anesth. Pain. Med. 2018, 43, 113–123. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Van de Velde, M.; Wouters, P.F.; Rolf, N.; Van Aken, H.; Vandermeersch, E. Comparative hemodynamic effects of three different parenterally administered lipid emulsions in conscious dogs. Crit. Care. Med. 1998, 26, 132–137. [Google Scholar] [CrossRef]
- Best, L.; Jarman, E.; Brown, P.D. A dual action of saturated fatty acids on electrical activity in rat pancreatic β-cells. Role of volume-regulated anion channel and KATP channel currents. J. Physiol. 2011, 589, 1307–1316. [Google Scholar] [CrossRef]
- Cole, W.C.; Malcolm, T.; Walsh, M.P.; Light, P.E. Inhibition by protein kinase C of the K(NDP) subtype of vascular smooth muscle ATP-sensitive potassium channel. Circ. Res. 2000, 87, 112–117. [Google Scholar] [CrossRef] [Green Version]
- Tousoulis, D.; Kampoli, A.M.; Tentolouris, C.; Papageorgiou, N.; Stefanadis, C. The role of nitric oxide on endothelial function. Curr. Vasc. Pharmacol. 2012, 10, 4–18. [Google Scholar] [CrossRef]
- Lee, L.; Webb, R.C. Endothelium-dependent relaxation and L-arginine metabolism in genetic hypertension. Hypertension 1992, 19, 435–441. [Google Scholar] [CrossRef] [Green Version]
- Hoegberg, L.C.; Bania, T.C.; Lavergne, V.; Bailey, B.; Turgeon, A.F.; Thomas, S.H.; Morris, M.; Miller-Nesbitt, A.; Mégarbane, B.; Magder, S.; et al. Lipid emulsion workgroup. Systematic review of the effect of intravenous lipid emulsion therapy for local anesthetic toxicity. Clin. Toxicol. (Phila) 2016, 54, 167–193. [Google Scholar] [CrossRef] [Green Version]
- Charbonneau, H.; Marcou, T.A.; Mazoit, J.X.; Zetlaoui, P.J.; Benhamou, D. Early use of lipid emulsion to treat incipient mepivacaine intoxication. Reg. Anesth. Pain. Med. 2009, 34, 277–278. [Google Scholar] [CrossRef]
- Zimmer, C.; Piepenbrink, K.; Riest, G.; Peters, J. Cardiotoxic and neurotoxic effects after accidental intravascular bupivacaine administration. Therapy with lidocaine propofol and lipid emulsion. Anaesthesist 2007, 56, 449–453. [Google Scholar] [CrossRef] [PubMed]
- Diaz, J.; Bernasinski, M.; Malinovsky, J.M. Reversal of neurologic symptoms related to lidocaine toxicity with a lipid emulsion administration. Ann. Fr. Anesth. Reanim. 2012, 31, 647. [Google Scholar] [CrossRef] [PubMed]
- Ludot, H.; Tharin, J.Y.; Belouadah, M.; Mazoit, J.X.; Malinovsky, J.M. Successful resuscitation after ropivacaine and lidocaine-induced ventricular arrhythmia following posterior lumbar plexus block in a child. Anesth. Analg. 2008, 106, 1572–1574. [Google Scholar] [CrossRef] [PubMed]
- Schellhammer, F.; Milde, A. Toxic lipid rescue therapy effect of local anesthesia in interventional radiology. Rofo 2011, 183, 73–74. [Google Scholar] [CrossRef]
- Lee, S.H.; Kang, D.; Ok, S.H.; Kwon, S.C.; Kim, H.J.; Kim, E.J.; Hong, J.M.; Kim, J.Y.; Bae, S.I.; An, S.; et al. Linoleic acid attenuates the toxic dose of bupivacaine-mediated reduction of vasodilation evoked by the activation of adenosine triphosphate-sensitive potassium channels. Int. J. Mol. Sci. 2018, 19, E1876. [Google Scholar] [CrossRef] [Green Version]
- Clifford, P.S. Local control of blood flow. Adv. Physiol. Educ. 2011, 35, 5–15. [Google Scholar] [CrossRef]
- Shin, I.W.; Hah, Y.S.; Kim, C.; Park, J.; Shin, H.; Park, K.E.; Ok, S.H.; Lee, H.K.; Chung, Y.K.; Shim, H.S.; et al. Systemic blockage of nitric oxide synthase by L-NAME increases left ventricular systolic pressure, which is not augmented further by Intralipid®. Int. J. Biol. Sci. 2014, 10, 367–376. [Google Scholar] [CrossRef] [Green Version]
- Lee, S.H.; Ok, S.H.; Kim, J.Y.; Subbarao, R.B.; Bae, S.I.; Hwang, Y.; Park, K.E.; Kim, J.W.; Sohn, J.T. Linolenic acid attenuates the vasodilation induced by acetylcholine in isolated rat aortae. Dose Response 2019, 17, 1559325819894148. [Google Scholar] [CrossRef] [Green Version]
- Klöss, S.; Bouloumié, A.; Mülsch, A. Aging and chronic hypertension decrease expression of rat aortic soluble guanylyl cyclase. Hypertension 2000, 35, 43–47. [Google Scholar] [CrossRef] [Green Version]
- Ogata, R.; Kitamura, K.; Ito, Y.; Nakano, H. Inhibitory effects of genistein on ATP-sensitive K+ channels in rabbit portal vein smooth muscle. Br. J. Pharmacol. 1997, 122, 1395–1404. [Google Scholar] [CrossRef] [Green Version]
- Lee, S.H.; Kwon, S.C.; Ok, S.H.; Hong, J.M.; Kim, J.Y.; Ahn, S.H.; Bae, S.I.; Shin, Y.; Sohn, J.T. Levobupivacaine-induced vasoconstriction involves caldesmon phosphorylation mediated by tyrosine kinase-induced ERK phosphorylation. Eur. J. Pharmacol. 2019, 842, 167–176. [Google Scholar] [CrossRef] [PubMed]
- Lavergne, C.; Martinez, M.; Trottier, C. Empirical model selection in generalized linear mixed effects models. Comput. Stat. 2008, 23, 99–109. [Google Scholar] [CrossRef]
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Share and Cite
Lee, S.H.; Kang, D.; Ok, S.-H.; Kim, J.-Y.; Bae, S.I.; Hwang, Y.; Park, K.-E.; Kim, J.W.; Sohn, J.-T. Lipofundin MCT/LCT Inhibits Levcromakalim-Induced Vasodilation by Inhibiting Endothelial Nitric Oxide Release. Int. J. Mol. Sci. 2020, 21, 1763. https://doi.org/10.3390/ijms21051763
Lee SH, Kang D, Ok S-H, Kim J-Y, Bae SI, Hwang Y, Park K-E, Kim JW, Sohn J-T. Lipofundin MCT/LCT Inhibits Levcromakalim-Induced Vasodilation by Inhibiting Endothelial Nitric Oxide Release. International Journal of Molecular Sciences. 2020; 21(5):1763. https://doi.org/10.3390/ijms21051763
Chicago/Turabian StyleLee, Soo Hee, Dawon Kang, Seong-Ho Ok, Ji-Yoon Kim, Sung Il Bae, Yeran Hwang, Kyeong-Eon Park, Jong Won Kim, and Ju-Tae Sohn. 2020. "Lipofundin MCT/LCT Inhibits Levcromakalim-Induced Vasodilation by Inhibiting Endothelial Nitric Oxide Release" International Journal of Molecular Sciences 21, no. 5: 1763. https://doi.org/10.3390/ijms21051763