Does the Pre-Ovulatory Pig Oviduct Rule Sperm Capacitation In Vivo Mediating Transcriptomics of Catsper Channels?
Abstract
:1. Introduction
2. Results
2.1. Gene Expression is Altered in the Pre-Ovulatory Oviduct after Semen or Sperm-Free SP Exposure
2.2. Commonly Altered Genes between Pre-Ovulatory Oviduct Segments after Statistical Restrictive Analysis within Each Experimental Group
2.3. Analysis of Functional Categories: Enriched Tubal Genes During Pre-Ovulation are Differentially Associated with Sperm Motility, Acrosome Reaction, Single Fertilization, and Regulation of Signal Transduction
2.4. Antagonistic Influences of Sperm- or Seminal Plasma on Pre-Ovulatory Oviductal Gene Expression for Sperm Capacitation Genes
3. Discussion
4. Materials and Methods
4.1. Experimental Design
4.2. Animal Management Including Ethics Statement
4.3. Semen Collection and SP Harvesting
4.4. Handling of Sows
4.5. Collection of Oviductal Samples
4.6. Transcriptome Analysis
4.7. Analysis of Microarray Data and Bioinformatics
4.8. Enrichment Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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MATING | |||||||
UTJ | ISTH | ||||||
Gene ID | Fold Change | p-value | Description | Gene ID | Fold Change | p-value | Description |
ABHD2 | −1.66 | 0.004 | Monoacylglycerol lipase protease | ABHD2 | −2.6 | 0.0003 | Monoacylglycerol lipase protease |
CATSPER2 | 1.53 | 0.01 | Cation channelsperm-associated protein 2 | CATSPERγ | 1.3 | 0.03 | Cation channelsperm-associated subunit gamma |
CATSPERγ | 1.56 | 0.006 | Cation channelsperm-associated subunit gamma | ING2 | 1.25 | 0.003 | Inhibitor of growth protein 2 |
GAS8 | 1.37 | 0.002 | Dynein regulatory complex subunit 8 | AAAS | −1.38 | 0.03 | Aladin WD repeat nucleoporin |
RLN2 | −1.16 | 0.02 | Prorelaxin precursor | B4GALT1 | −1.89 | 0.0005 | Beta-1,4-galactosyltransferase |
TEKT2 | 1.62 | 0.03 | Tektin-2 non-motor microtubule binding protein | BCL2L1 | 1.28 | 0.009 | Anti-apoptotic signaling molecule |
TEKT3 | 1.45 | 0.04 | Tektin-3 non-motor microtubule binding protein | CCT3 | −1.19 | 0.04 | T-complex protein 1 subunit gamma |
B4GALT1 | −1.69 | 0.0009 | Beta-1,4-galactosyltransferase | EHMT2 | 1.18 | 0.02 | Histone-Lysine N-methyltransferase |
BCL2L1 | 1.37 | 0.007 | Anti-apoptotic signaling molecule | MFGE8 | −4.37 | <0.0001 | Lactadherin membrane-bound signaling molecule |
MFGE8 | −2.23 | 0.004 | Lactadherin membrane-bound signaling molecule | NR2F2 | 1.73 | 0.001 | C4 Zinc finger nuclear binding receptor |
RNASE10 | −1.15 | 0.03 | Inactive ribonuclease-like protein 10 | SPINK2 | 1.33 | 0.03 | Serine protease inhibitor Kazal type 2 |
SPAM1 | −1.21 | 0.04 | Sperm adhesión member | TARBP2 | −1.26 | 0.004 | RISC-loading complex subunit 2 |
SPEF2 | 1.61 | 0.03 | Sperm flagelar protein 2 | ZP4 | −4.09 | <0.0001 | Zona pellucida sperm-binding protein 4 |
TARBP2 | −1.47 | 0.001 | RISC-loading complex subunit 2 | AKAP11 | 1.18 | 0.04 | A-Kinase Anchor protein 11 |
TRPC3 | 1.71 | 0.003 | Short transitient receptor potential channel 3 | AKAP12 | 1.55 | 0.02 | A-Kinase Anchor protein 12 |
ZP4 | −1.76 | 0.03 | Zona pellucida sperm-binding protein 4 | PKIA | −3.4 | 0.0002 | cAMP-dependent kinase inhibitor alpha |
AKAP11 | 1.43 | 0.007 | A-Kinase Anchor protein 11 | CABYR | 1.22 | 0.01 | Calcium-binding tyrosine phosphorylation-regulated protein |
AKAP13 | 1.37 | 0.007 | A-Kinase Anchor protein 13 | ||||
PKIA | −2.33 | 0.02 | cAMP-dependent kinase inhibitor alpha | ||||
CABYR | 1.33 | 0.007 | Calcium-binding tyrosine phosphorylation-regulated protein | ||||
P1-AI | |||||||
UTJ | ISTH | ||||||
CACNA1I | 1.56 | 0.006 | Voltage-dependent L-type calcium channel | ACRBP | 1.38 | 0.04 | Acrosin-binding protein |
CFTR | −2.38 | 0.009 | ATP-binding cassette transporter | CATSPERγ | 1.42 | 0.008 | Cation channelsperm-associated subunit gamma |
DPCD | −1.49 | 0.04 | Deleted in primary ciliary dyskinesia | ING2 | 1.17 | 0.03 | Inhibitor of growth protein 2 |
SPAG6 | −2.58 | 0.03 | Sperm-associated antigen 6 | AQN-1 | −1.336 | 0.04 | Carbohydrate-binding protein |
HOXD9 | 1.52 | 0.02 | Homeobox protein 9 | PRDM14 | 1.19 | 0.04 | PR domain Zinc finger protein 14 |
HOXD10 | 2.42 | 0.04 | Homeobox protein 10 | TARBP2 | −1.18 | 0.01 | RISC-loading complex subunit 2 |
SPA17 | −1.7 | 0.03 | Sperm Surface protein | ZP4 | −3.01 | 0.0007 | Zona pellucida sperm-binding protein |
TARBP2 | −1.25 | 0.02 | RISC-loading complex subunit 2 | ||||
TRPC3 | 1.65 | 0.01 | Short transient receptor potential cannel 3 | ||||
AKAP11 | 1.57 | 0.009 | A-Kinase Anchor protein 11 | ||||
AKAP13 | 1.4 | 0.04 | A-Kinase Anchor protein 13 | ||||
PKIB | −1.81 | 0.01 | cAMP-dependent kinase inhibitor beta |
SP-P1 | |||||||
UTJ | ISTH | ||||||
Gene ID | Fold Change | p-value | Description | Gene ID | Fold Change | p-value | Description |
INSL6 | 1.39 | 0.0007 | Insulin-like 6 peptide | CACNA1D | 1.65 | 0.0008 | Voltage-dependent L-type calcium channel |
RNASE10 | −1.21 | 0.02 | Inactive ribonuclease-like protein 10 | TEKT3 | −1.14 | 0.04 | Tektin-3 non-motor microtubule binding protein |
SLC26A8 | 1.28 | 0.02 | Anion transporter 1 | EHHMT2 | 1.16 | 0.04 | Histone-Lysine N-methyltransferase |
CRISP1 | 1.3 | 0.04 | Custein-rich secretory protein 1 | HOXA11 | −1.31 | 0.02 | Homeobox protein A-11 |
PLCZ1 | 1.17 | 0.02 | Calcium-binding protein phospholipase signaling molecule | SYCP2 | 1.92 | 0.01 | Synaptonemal complexprotein 2 |
TRPC3 | 1.45 | 0.04 | Short transient receptor potential cannel 3 | TARBP2 | −1.33 | 0.0007 | RISC-loading complex subunit 2 |
SP-Ejac | |||||||
UTJ | ISTH | ||||||
CATSPER1 | −1.37 | 0.01 | Cation channelsperm-associated protein 1 | AAAS | 1.16 | 0.04 | Aladin WD repeat nucleoporin |
RLN2 | −1.22 | 0.04 | Prorelaxin precursor | ||||
RNASE10 | −1.16 | 0.03 | Inactive ribonuclease-like protein 10 |
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Martinez, C.A.; Alvarez-Rodriguez, M.; Wright, D.; Rodriguez-Martinez, H. Does the Pre-Ovulatory Pig Oviduct Rule Sperm Capacitation In Vivo Mediating Transcriptomics of Catsper Channels? Int. J. Mol. Sci. 2020, 21, 1840. https://doi.org/10.3390/ijms21051840
Martinez CA, Alvarez-Rodriguez M, Wright D, Rodriguez-Martinez H. Does the Pre-Ovulatory Pig Oviduct Rule Sperm Capacitation In Vivo Mediating Transcriptomics of Catsper Channels? International Journal of Molecular Sciences. 2020; 21(5):1840. https://doi.org/10.3390/ijms21051840
Chicago/Turabian StyleMartinez, Cristina A., Manuel Alvarez-Rodriguez, Dominic Wright, and Heriberto Rodriguez-Martinez. 2020. "Does the Pre-Ovulatory Pig Oviduct Rule Sperm Capacitation In Vivo Mediating Transcriptomics of Catsper Channels?" International Journal of Molecular Sciences 21, no. 5: 1840. https://doi.org/10.3390/ijms21051840