Perinuclear Lamin A and Nucleoplasmic Lamin B2 Characterize Two Types of Hippocampal Neurons through Alzheimer’s Disease Progression
Abstract
:1. Introduction
2. Results
2.1. Phosphorylated Tau Shifts from Nucleus to Cytosol through AD Progression
2.2. Hippocampal Neurons Express Lamin A from Early to Late AD Stages
2.3. Lamin B2 Redistributes in the Nucleoplasm along AD Stages
2.4. Nuclear Lamin and Nuclear Tau Changes are associated with Constitutive Heterochromatin Modifications
2.5. Lamin A Expression in Cells with Perinuclear Lamin B2
3. Discussion
3.1. Neurons Express Lamin A from Early AD Stages on
3.2. Lamin B2 in the Nucleoplasm of AD Neurons
3.3. Lamin A Associated Epigenetic Changes at Early AD Stages
3.4. Nuclear AT8 in Aging Cycling Neurons
3.5. Lamin A and Tau Oligomers in Neurons with NFTs
3.6. Methodology
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Antibody/Supplier/Catalog Number/Manufacture | Species | Dilution |
---|---|---|
AT100 (antiphospho Tau S212-T214)/abcam/Thermo Fisher/MN1060/Waltham, MA, USA | mouse monoclonal | 1:100 |
AT8 (antiphospho Tau S205-T205)/Thermo Fisher/MN1020/Waltham, MA, USA | mouse monoclonal | 1:20 |
AH36 (antiphospho Tau S205-T205) Stress Marq/SMC-601/Victoria, B.C., Canada | rabbit monoclonal | 1:100 |
Anti-Lamin A/abcam/ab26300/Cambridge, UK | rabbit polyclonal | 1:500 |
Anti-Lamin B1/abcam/ab133741/Cambridge, UK | rabbit monoclonal | 1:200 |
Anti-Lamin B2/abcam/ab151735/Cambridge, UK | rabbit monoclonal | 1:200 |
Anti-Lamin C/abcam/ab125679/Cambridge, UK | rabbit polyclonal | 1:20 |
B23 (anti-nucleophosmin)/abcam/ab10530/Cambridge, UK | mouse monoclonal | 1:50 |
Anti- H4K20me3/abcam/ab9053/Cambridge, UK | rabbit monoclonal | 1:100 |
Alexa –Fluor 488 secondary antibody/Life Technologies/CA, USA | goat anti-rabbit | 1:300 |
Alexa –Fluor 633 secondary antibody/Life Technologies/Carlsbad, CA, USA | goat anti-mouse | 1:200 |
Sytox Orange nucleic acid stain/Molecular Probes/Eugene, OR, USA | 1:1000 |
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Gil, L.; Niño, S.A.; Chi-Ahumada, E.; Rodríguez-Leyva, I.; Guerrero, C.; Rebolledo, A.B.; Arias, J.A.; Jiménez-Capdeville, M.E. Perinuclear Lamin A and Nucleoplasmic Lamin B2 Characterize Two Types of Hippocampal Neurons through Alzheimer’s Disease Progression. Int. J. Mol. Sci. 2020, 21, 1841. https://doi.org/10.3390/ijms21051841
Gil L, Niño SA, Chi-Ahumada E, Rodríguez-Leyva I, Guerrero C, Rebolledo AB, Arias JA, Jiménez-Capdeville ME. Perinuclear Lamin A and Nucleoplasmic Lamin B2 Characterize Two Types of Hippocampal Neurons through Alzheimer’s Disease Progression. International Journal of Molecular Sciences. 2020; 21(5):1841. https://doi.org/10.3390/ijms21051841
Chicago/Turabian StyleGil, Laura, Sandra A. Niño, Erika Chi-Ahumada, Ildelfonso Rodríguez-Leyva, Carmen Guerrero, Ana Belén Rebolledo, José A. Arias, and María E. Jiménez-Capdeville. 2020. "Perinuclear Lamin A and Nucleoplasmic Lamin B2 Characterize Two Types of Hippocampal Neurons through Alzheimer’s Disease Progression" International Journal of Molecular Sciences 21, no. 5: 1841. https://doi.org/10.3390/ijms21051841
APA StyleGil, L., Niño, S. A., Chi-Ahumada, E., Rodríguez-Leyva, I., Guerrero, C., Rebolledo, A. B., Arias, J. A., & Jiménez-Capdeville, M. E. (2020). Perinuclear Lamin A and Nucleoplasmic Lamin B2 Characterize Two Types of Hippocampal Neurons through Alzheimer’s Disease Progression. International Journal of Molecular Sciences, 21(5), 1841. https://doi.org/10.3390/ijms21051841