Rice OsHSFA3 Gene Improves Drought Tolerance by Modulating Polyamine Biosynthesis Depending on Abscisic Acid and ROS Levels
Abstract
:1. Introduction
2. Results
2.1. OsHSFA3 Is a Drought-Responsive Gene and Highly Accumulated in Shoot
2.2. Structural Features of OsHSFA3 and Evolutionary Relationship
2.3. OsHSFA3 Localized in Nucleus
2.4. Overexpression of OsHSFA3 Increases Osmotic and Drought Stress Tolerance in Transgenic Arabidopsis
2.5. Overexpression of OsHSFA3 Increases Drought Tolerance by Decreasing H2O2 Accumulation and Increasing ABA Levels
2.6. OsHSFA3 Regulates Polyamines Biosynthesis under Drought Stress
2.7. Expression of Polyamine Biosynthesis Genes is Induced under Drought Stress
2.8. OsHSFA3 Interacts with OsADC1 and AtADC1 Promoter
3. Discussion
4. Materials and Methods
4.1. Plant Growth Conditions and Stress Treatments
4.2. Total RNA Isolation and qRT-PCR
4.3. Gene structure Prediction and Phylogenetic Analysis
4.4. Vector Construction and Transformation
4.5. Subcellular Localization
4.6. Measurement of ROS, MDA and Antioxidant Activities
4.7. Analysis of Polyamines and ABA
4.8. Yeast One Hybrid Assay
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Zhu, M.-D.; Zhang, M.; Gao, D.-J.; Zhou, K.; Tang, S.-J.; Zhou, B.; Lv, Y.-M. Rice OsHSFA3 Gene Improves Drought Tolerance by Modulating Polyamine Biosynthesis Depending on Abscisic Acid and ROS Levels. Int. J. Mol. Sci. 2020, 21, 1857. https://doi.org/10.3390/ijms21051857
Zhu M-D, Zhang M, Gao D-J, Zhou K, Tang S-J, Zhou B, Lv Y-M. Rice OsHSFA3 Gene Improves Drought Tolerance by Modulating Polyamine Biosynthesis Depending on Abscisic Acid and ROS Levels. International Journal of Molecular Sciences. 2020; 21(5):1857. https://doi.org/10.3390/ijms21051857
Chicago/Turabian StyleZhu, Ming-Dong, Meng Zhang, Du-Juan Gao, Kun Zhou, Shan-Jun Tang, Bin Zhou, and Yan-Mei Lv. 2020. "Rice OsHSFA3 Gene Improves Drought Tolerance by Modulating Polyamine Biosynthesis Depending on Abscisic Acid and ROS Levels" International Journal of Molecular Sciences 21, no. 5: 1857. https://doi.org/10.3390/ijms21051857