LINC00312/YBX1 Axis Regulates Myofibroblast Activities in Oral Submucous Fibrosis
Abstract
:1. Introduction
2. Results
2.1. LINC00312 is Upregulated in OSF Tissues and Associated with Fibrosis Markers
2.2. Suppression of LINC00312 in fBMFs Downregulates the Myofibroblast Features
2.3. YBX1 is a Putative Target of LINC00312 and Increased in the OSF Specimens
2.4. Inhibition of YBX1 ameliorates the characteristics of fBMFs
2.5. LINC00312 Enhances the Myofibroblast Activities via YBX1
3. Discussion
4. Materials and Methods
4.1. Cell Culture for Buccal Mucosa Fibroblasts (BMFs) and Fibrotic Buccal Mucosa Fibroblasts (fBMFs)
4.2. Tissues Acquirements
4.3. Quantitative Real-Time PCR (qRT-PCR)
4.4. RNA Sequencing
4.5. Lentiviral-Mediated Silencing of LINC00312 and YBX1
4.6. Collagen Gel Contraction Assay
4.7. Western Blot Analysis
4.8. Transwell Migration and Invasion Assay
4.9. Wound Healing Assay
4.10. Overexpression of LINC00312
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Yu, C.-H.; Fang, C.-Y.; Yu, C.-C.; Hsieh, P.-L.; Liao, Y.-W.; Tsai, L.-L.; Chu, P.-M. LINC00312/YBX1 Axis Regulates Myofibroblast Activities in Oral Submucous Fibrosis. Int. J. Mol. Sci. 2020, 21, 2979. https://doi.org/10.3390/ijms21082979
Yu C-H, Fang C-Y, Yu C-C, Hsieh P-L, Liao Y-W, Tsai L-L, Chu P-M. LINC00312/YBX1 Axis Regulates Myofibroblast Activities in Oral Submucous Fibrosis. International Journal of Molecular Sciences. 2020; 21(8):2979. https://doi.org/10.3390/ijms21082979
Chicago/Turabian StyleYu, Chuan-Hang, Chih-Yuan Fang, Cheng-Chia Yu, Pei-Ling Hsieh, Yi-Wen Liao, Lo-Lin Tsai, and Pei-Ming Chu. 2020. "LINC00312/YBX1 Axis Regulates Myofibroblast Activities in Oral Submucous Fibrosis" International Journal of Molecular Sciences 21, no. 8: 2979. https://doi.org/10.3390/ijms21082979