Yeast Translation Elongation Factor eIF5A Expression Is Regulated by Nutrient Availability through Different Signalling Pathways
Abstract
:1. Introduction
2. Results
2.1. The Tif51A Isoform of Yeast eIF5A Is Required for Respiration and Growth with Non-Fermentative Carbon Sources
2.2. Snf1 and Hap1 Signalling Pathways Are Involved in TIF51A Induction under Respiratory Conditions
2.3. TIF51A Expression Drops during the Diauxic Shift But Subsequently Increases in a Hap1-Dependent Manner
2.4. Glucose Availability and TORC1 Regulate eIF5A Expression
2.5. An Increase in the Metabolic Flux in the TCA Cycle and at Heme Cellular Levels Up-Regulates TIF51A in a Hap1-Dependent Manner
2.6. TIF51A Is Regulated under Iron Deficiency in a Hap1-Dependent Manner
3. Discussions
4. Materials and Methods
4.1. Yeast Strains and Growth Conditions
4.2. Western Blot Analysis
4.3. RT-qPCR Analysis
4.4. Oxygen Consumption Assays
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
eIF2A | Eukaryotic translation initiation factor 2A |
eIF5A | Eukaryotic translation initiation factor 5A |
ETC | Electron transport chain |
OXPHOS | Oxidative phosphorylation |
PKA | Protein kinase A |
ROS | Reactive oxygen species |
SDH | Succinate dehydrogenase |
TCA | Tricarboxylic acid |
TORC1 | Target of rapamycin complex I |
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Barba-Aliaga, M.; Villarroel-Vicente, C.; Stanciu, A.; Corman, A.; Martínez-Pastor, M.T.; Alepuz, P. Yeast Translation Elongation Factor eIF5A Expression Is Regulated by Nutrient Availability through Different Signalling Pathways. Int. J. Mol. Sci. 2021, 22, 219. https://doi.org/10.3390/ijms22010219
Barba-Aliaga M, Villarroel-Vicente C, Stanciu A, Corman A, Martínez-Pastor MT, Alepuz P. Yeast Translation Elongation Factor eIF5A Expression Is Regulated by Nutrient Availability through Different Signalling Pathways. International Journal of Molecular Sciences. 2021; 22(1):219. https://doi.org/10.3390/ijms22010219
Chicago/Turabian StyleBarba-Aliaga, Marina, Carlos Villarroel-Vicente, Alice Stanciu, Alba Corman, María Teresa Martínez-Pastor, and Paula Alepuz. 2021. "Yeast Translation Elongation Factor eIF5A Expression Is Regulated by Nutrient Availability through Different Signalling Pathways" International Journal of Molecular Sciences 22, no. 1: 219. https://doi.org/10.3390/ijms22010219