Deletion of KLF10 Leads to Stress-Induced Liver Fibrosis upon High Sucrose Feeding
Abstract
:1. Introduction
2. Results
2.1. Klf10 Deletion Induces High-Sucrose Diet-Mediated Liver Injury
2.2. Klf10 Deletion Impairs Triglyceride Packaging and Secretion upon HSD Feeding
2.3. Klf10 Deletion Induces Oxidative Stress and ER Stress upon HSD Feeding
2.4. Klf10 Deletion Increases Stress-Mediated Hepatic Cell Death upon HSD Feeding
2.5. Klf10 Deletion Induces Liver Fibrosis via the Activation of the TGF-β- Smad3 Pathway
3. Discussion
4. Materials and Methods
4.1. Mice and Diets
4.2. Plasma and Tissue Biochemical Analysis
4.3. Histological Evaluation
4.4. In Vivo VLDL Secretion
4.5. RNA Isolation and Quantitative Real-Time Polymerase Chain Reaction
4.6. Preparation of Nuclear and Cytoplasmic Proteins from Cells and Tissues
4.7. Western Blotting
4.8. Preparation of Mouse Hepatocytes and Cell Viability Assay
4.9. TUNEL Assay
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
CD | Control chow diet |
CHOP | C/EBP homologous protein |
eIF2α | Eukaryotic translation initiation factor 2α |
ER | Endoplasmic reticulum |
HSD | High-sucrose diet |
JNK | c-Jun-N-terminal kinase |
KLF10 | Krüppel-like factor 10 |
KO | Knockout |
MTTP | Microsomal triglyceride transfer protein |
NAFLD | Nonalcoholic fatty liver diseases |
NASH | Nonalcoholic steatohepatitis |
PERK | Protein kinase R-like ER kinase |
TGF-β | Transforming growth factor-beta |
VLDL | Very-low-density lipoprotein |
WT | Wild type |
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Lee, J.; Oh, A.-R.; Lee, H.-Y.; Moon, Y.-A.; Lee, H.-J.; Cha, J.-Y. Deletion of KLF10 Leads to Stress-Induced Liver Fibrosis upon High Sucrose Feeding. Int. J. Mol. Sci. 2021, 22, 331. https://doi.org/10.3390/ijms22010331
Lee J, Oh A-R, Lee H-Y, Moon Y-A, Lee H-J, Cha J-Y. Deletion of KLF10 Leads to Stress-Induced Liver Fibrosis upon High Sucrose Feeding. International Journal of Molecular Sciences. 2021; 22(1):331. https://doi.org/10.3390/ijms22010331
Chicago/Turabian StyleLee, Junghoon, Ah-Reum Oh, Hui-Young Lee, Young-Ah Moon, Ho-Jae Lee, and Ji-Young Cha. 2021. "Deletion of KLF10 Leads to Stress-Induced Liver Fibrosis upon High Sucrose Feeding" International Journal of Molecular Sciences 22, no. 1: 331. https://doi.org/10.3390/ijms22010331