Perinatal Gram-Positive Bacteria Exposure Elicits Distinct Cytokine Responses In Vitro
Abstract
:1. Introduction
2. Results
2.1. Bacterial Lysates Trigger the Secretion of Cytokines and Chemokines by Umbilical Cord Blood Mononuclear Cells
2.2. The Bacterial Lysates Elicit Distinct Patterns of Cytokines Responses
2.3. Umbilical Blood Mononuclear Cells from Female Infants Respond More Pronouncedly to Bacterial Lysates than Those from Male Infants
2.4. IL-8 Levels in Cord Blood of Preterm Newborn Infants is a Stable Indicator of Perinatal Infections
3. Discussion
4. Materials and Methods
4.1. Umbilical Cord Blood Source
4.2. Isolation of Umbilical Cord Blood Mononuclear Cells (UBMC)
4.3. Preparation of Bacterial Lysates
4.4. Stimulation of UBMC with Gram-Positive Bacterial Lysates
4.5. Determination of Cytokine and Chemokine Concentrations in UBMC Supernatants
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Subject Number | Maternal Age (Years) | Parity | Gestational Age (Weeks + Days) | Maternal BMI Before Pregnancy | Neonatal Birth Weight (g) | Neonatalsex |
---|---|---|---|---|---|---|
1 | 31 | 6 | 40 + 3 | 25.9 | 3810 | Male |
2 | 29 | 2 | 41 + 0 | 24.1 | 3540 | Female |
3 | 33 | 1 | 39 + 4 | 31.9 | 4150 | Female |
4 | 29 | 2 | 40 + 6 | 30.8 | 3740 | Male |
5 | 27 | 1 | 40 + 1 | 22.1 | 3150 | Female |
6 | 34 | 1 | 40 + 5 | 35.0 | 3300 | Male |
7 | 25 | 2 | 38 + 5 | 32.4 | 3800 | Male |
8 | 27 | 1 | 39 + 4 | 22.5 | 3620 | Male |
9 | 30 | 2 | 37 + 1 | 21.5 | 2830 | Female |
10 | 27 | 1 | 37 + 0 | 22.9 | 2570 | Female |
Median | 29 | 1.5 | 39 + 3 | 25.0 | 3580 | |
Range | 25–31 | 1–6 | 37–41 | 21.5–35.0 | 2570–4150 |
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Reuschel, E.; Toelge, M.; Haeusler, S.; Deml, L.; Seelbach-Goebel, B.; Solano, M.E. Perinatal Gram-Positive Bacteria Exposure Elicits Distinct Cytokine Responses In Vitro. Int. J. Mol. Sci. 2021, 22, 332. https://doi.org/10.3390/ijms22010332
Reuschel E, Toelge M, Haeusler S, Deml L, Seelbach-Goebel B, Solano ME. Perinatal Gram-Positive Bacteria Exposure Elicits Distinct Cytokine Responses In Vitro. International Journal of Molecular Sciences. 2021; 22(1):332. https://doi.org/10.3390/ijms22010332
Chicago/Turabian StyleReuschel, Edith, Martina Toelge, Sebastian Haeusler, Ludwig Deml, Birgit Seelbach-Goebel, and Maria Emilia Solano. 2021. "Perinatal Gram-Positive Bacteria Exposure Elicits Distinct Cytokine Responses In Vitro" International Journal of Molecular Sciences 22, no. 1: 332. https://doi.org/10.3390/ijms22010332