Vanillic Acid, a Bioactive Phenolic Compound, Counteracts LPS-Induced Neurotoxicity by Regulating c-Jun N-Terminal Kinase in Mouse Brain
Abstract
:1. Introduction
2. Results
2.1. Neuroprotective Effect of Vanillic Acid on Expression of LPS-Elevated RAGE Protein and Activated Gliosis in Mice Brains
2.2. Neuroprotective Effect of Vanillic Acid on Expression of LPS-Activated p-JNK Protein in Mice Brains
2.3. Neuroprotective Effect of Vanillic Acid on LPS-Mediated p-NF-κB Activation and Associated Neuroinflammatory Markers/Cytokines in Mice Brains
2.4. Neuroprotective Effect of Vanillic Acid on Expression of LPS-Elevated BACE-1 and Aβ Proteins in Mice Brains
2.5. Neuroprotective Effect of Vanillic Acid on LPS-Induced Synaptic Dysfunction and Memory Impairment in Mice Brains
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Mouse Strain, Acclimatization/Housing and Ethical Aspects
4.3. Study Designing, Experimental Animals Grouping, and Their Drug Treatment
- Normal saline-treated (control) group: Mice treated with normal saline (i.p. 0.9% saline for 14 days).
- Lipopolysaccharide-alone treated group: Mice treated with LPS (i.p. 250 μg/kg/day, for 7 days).
- LPS + vanillic acid-co-treated group: Mice treated with LPS (i.p. 250 μg/kg/day) for 7 days and V.A (i.p. 30 mg/kg for 14 days (7 days prior to the LPS and 7 days co-treated with LPS).
- V.A-alone treated group: Mice treated with V.A (i.p. 30 mg/kg for 14 days alone (V.A).
4.4. Behavioral Tests
4.5. Protein Extraction for Biochemical Analysis from Mice Brains
4.6. Western Blot Analysis
4.7. Brain Tissue Collection and Sample Preparation for the Immunofluorescence Staining
4.8. Immunofluorescence Staining
4.9. Antibodies
4.10. Data and Statistical Analyses
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
LPS | Lipopolysaccharide |
V.A | Vanillic acid |
CNS | Central nervous system |
p-JNK | c-Jun n-terminal kinase |
NF-KB | nuclear factor kappa-light-chain-enhancer of activated B cells |
TNF-α | tumor necrosis factor alpha |
IL1-β | interleukin-1 β |
COX-2 | cyclooxygenase |
Aβ | amyloid-β |
BACE1 | β-site amyloid precursor protein (APP)–cleaving enzyme 1 |
PSD-95 | Post-synaptic density protein 95 |
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Antibody | Host | Catalog | Application | Dilution | Manufacturer |
---|---|---|---|---|---|
Anti-β-Actin Antibody | Mouse | sc-47778 | WB | 1:1000 | Santa Cruz Biotechnology (Dallas, TX, USA) |
Anti-p-JNK Antibody | Mouse | sc-6254 | WB and IF | 1:1000/1:100 | Santa Cruz Biotechnology (Dallas, TX, USA) |
Anti-PSD-95 Antibody | Mouse | sc-71933 | WB and IF | 1:1000/1:100 | Santa Cruz Biotechnology (Dallas, TX, USA) |
Anti-Synaptophysin | Rabbit | sc-7568 | WB | 1:1000 | Santa Cruz Biotechnology (Dallas, TX, USA) |
Anti-BACE-1 Antibody | Mouse | sc-33711 | WB | 1:1000 | Santa Cruz Biotechnology (Dallas, TX, USA) |
Anti-Aβ Antibody | Mouse | sc-28365 | WB and IF | 1:1000/1:100 | Santa Cruz Biotechnology (Dallas, TX, USA) |
Anti-Iba-1 Antibody | Mouse | sc-32725 | WB | 1:1000 | Santa Cruz Biotechnology (Dallas, TX, USA) |
Anti-GFAP Antibody | Mouse | sc-33673 | WB and IF | 1:1000/1:100 | Santa Cruz Biotechnology (Dallas, TX, USA) |
Anti-IL-1βeta Antibody | Mouse | sc-7884 | WB and IF | 1:1000/1:100 | Santa Cruz Biotechnology (Dallas, TX, USA) |
Anti-COX-2 Antibody | Rabbit | SC: 7951 | WB | 1:1000 | Santa Cruz Biotechnology (Dallas, TX, USA) |
Anti-TNF-α Antibody | Mouse | sc-52746 | WB | 1:1000 | Santa Cruz Biotechnology (Dallas, TX, USA) |
Anti-RAGE Antibody | Mouse | sc-80652 | WB and IF | 1:1000/1:100 | Santa Cruz Biotechnology (Dallas, TX, USA) |
Anti-p-NF-Κb Antibody | Mouse/Rabbit | SC 8008 | WB and IF | 1:1000/1:100 | Santa Cruz Biotechnology (Dallas, TX, USA) |
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Ullah, R.; Ikram, M.; Park, T.J.; Ahmad, R.; Saeed, K.; Alam, S.I.; Rehman, I.U.; Khan, A.; Khan, I.; Jo, M.G.; et al. Vanillic Acid, a Bioactive Phenolic Compound, Counteracts LPS-Induced Neurotoxicity by Regulating c-Jun N-Terminal Kinase in Mouse Brain. Int. J. Mol. Sci. 2021, 22, 361. https://doi.org/10.3390/ijms22010361
Ullah R, Ikram M, Park TJ, Ahmad R, Saeed K, Alam SI, Rehman IU, Khan A, Khan I, Jo MG, et al. Vanillic Acid, a Bioactive Phenolic Compound, Counteracts LPS-Induced Neurotoxicity by Regulating c-Jun N-Terminal Kinase in Mouse Brain. International Journal of Molecular Sciences. 2021; 22(1):361. https://doi.org/10.3390/ijms22010361
Chicago/Turabian StyleUllah, Rahat, Muhammad Ikram, Tae Ju Park, Riaz Ahmad, Kamran Saeed, Sayed Ibrar Alam, Inayat Ur Rehman, Amjad Khan, Ibrahim Khan, Min Gi Jo, and et al. 2021. "Vanillic Acid, a Bioactive Phenolic Compound, Counteracts LPS-Induced Neurotoxicity by Regulating c-Jun N-Terminal Kinase in Mouse Brain" International Journal of Molecular Sciences 22, no. 1: 361. https://doi.org/10.3390/ijms22010361