Protein Sensing Device with Multi-Recognition Ability Composed of Self-Organized Glycopeptide Bundle
Abstract
:1. Introduction
2. Results and Discussion
2.1. Fabrication of Protein Sensing Systems Composed of Target Protein-Induced Self-Organized Glycopeptide Bundle
2.2. Structure of Glycopeptide Bundle Induced by Target Protein in the Lipid Bilayer Membrane
2.3. Structure of Bilayer Membrane Containing Protein-Induced Glycopeptide Bundle on the Electrode Surface
2.3.1. Conformation and Orientation of the Peptides in the Protein-Induced Glycopeptide Bundles Fixed on the Electrodes
2.3.2. Structure of Fixed Bilayer Membranes on the Electrodes
2.4. Recognition Ability of the Target Protein to the Sensing Membrane by QCM Method
2.5. Signal Transduction Ability of the Target Protein Binding to the Sensing Membrane by Electrochemical Method
2.6. Difference in Protein Recognition Ability by the QCM and Electrochemical Method
2.7. The Potential for Selective Protein Detection
3. Materials and Methods
3.1. Materials
3.1.1. Glycopeptides
3.1.2. Target Proteins
3.1.3. Vesicles
3.1.4. Formation of Glycopeptide Bundle and Fix on the Electrode
3.2. Methods
3.2.1. TEM Observations
3.2.2. FTIR-RAS Measurements
3.2.3. QCM Measurements
3.2.4. Electrochemical Measurements
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Appendix A
References
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ΔF/Hz | W/ng | Coverage/% | |
---|---|---|---|
DPPC | 786.0 | 23.6 *1. | 91.9 |
PNA-induced glycopeptide bundle/DPPC | 874.8 | 26.0 *2. | 104 |
ConA-induced glycopeptide bundle/DPPC | 859.4 | 25.5 *2. | 102 |
QCM method | PNA-induced glycopeptide bundle in DPPC bilayer membrane | |||||
PNA | ConA | BSA | ||||
K/M−1 | Wmax/ng | K/M−1 | Wmax/ng | K/M−1 | Wmax/ng | |
8.2 × 108 | 0.47 | 1.1 × 109 | 0.34 | 3.1 × 109 | 0.19 | |
ConA-induced glycopeptide bundle in DPPC bilayer, embrane | ||||||
PNA | ConA | BSA | ||||
K/M−1 | Wmax/ng | K/M−1 | Wmax/ng | K/M−1 | Wmax/ng | |
9.5 × 108 | 0.21 | 1.2 × 109 | 0.31 | 6.7 × 108 | 0.23 | |
Electrochemical Method | PNA-induced glycopeptide bundle in DPPC bilayer membrane | |||||
PNA | ConA | BSA | ||||
K/M−1 | ΔImax/μA | K/M−1 | ΔImax/μA | K/M−1 | ΔImax/μA | |
2.1 × 109 | 0.41 | 7.4 × 108 | 0.043 | 9.5 × 108 | 0.023 | |
ConA-induced glycopeptide bundle in DPPC bilayer membrane | ||||||
PNA | ConA | BSA | ||||
K/M−1 | ΔImax/μA | K/M−1 | ΔImax/μA | K/M−1 | ΔImax/μA | |
1.1 × 109 | 0.035 | 1.6 × 109 | 0.26 | 5.8 × 108 | 0.028 |
QCM method | PNA-induced glycopeptide bundle in DPPC bilayer membrane | |
PNA to ConA | PNA to BSA | |
1.34 | 2.47 | |
ConA-induced glycopeptide bundle in DPPC bilayer, embrane | ||
ConA to PNA | ConA to BSA | |
1.42 | 1.34 | |
Electrochemical Method | PNA-induced glycopeptide bundle in DPPC bilayer membrane | |
PNA to ConA | PNA to BSA | |
9.53 | 17.8 | |
ConA-induced glycopeptide bundle in DPPC bilayer membrane | ||
ConA to PNA | ConA to BSA | |
7.49 | 9.35 |
Binding Constant and Saturated Signal Value | |||||
---|---|---|---|---|---|
PNA | ABA | RCA120 | |||
K/M−1 | ΔImax/μA | K/M−1 | ΔImax/μA | K/M−1 | ΔImax/μA |
2.1 × 109 | 0.41 | 1.8 × 109 | 0.21 | 1.1 × 109 | 0.11 |
Selectivity of PNA to other proteins | |||||
PNA to ABA | PNA to RCA120 | ||||
1.95 | 3.76 |
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Arai, M.; Miura, T.; Ito, Y.; Kinoshita, T.; Higuchi, M. Protein Sensing Device with Multi-Recognition Ability Composed of Self-Organized Glycopeptide Bundle. Int. J. Mol. Sci. 2021, 22, 366. https://doi.org/10.3390/ijms22010366
Arai M, Miura T, Ito Y, Kinoshita T, Higuchi M. Protein Sensing Device with Multi-Recognition Ability Composed of Self-Organized Glycopeptide Bundle. International Journal of Molecular Sciences. 2021; 22(1):366. https://doi.org/10.3390/ijms22010366
Chicago/Turabian StyleArai, Mao, Tomohiro Miura, Yuriko Ito, Takatoshi Kinoshita, and Masahiro Higuchi. 2021. "Protein Sensing Device with Multi-Recognition Ability Composed of Self-Organized Glycopeptide Bundle" International Journal of Molecular Sciences 22, no. 1: 366. https://doi.org/10.3390/ijms22010366