eIF3a Destabilization and TDP-43 Alter Dynamics of Heat-Induced Stress Granules
Abstract
:1. Introduction
2. Results
2.1. eIF3a/Rpg1 Variant Induces Accumulation of the eIF3 Complex in Foci at Moderate Heat Shock
2.2. Rpg1-3 Foci Arising at Moderate Heat Stress Contain Translation Machinery Components but Not Ribosomal Subunits
2.3. Rpg1-3 Foci Formed at 42 °C Include mRNAs and Associate with P-Bodies
2.4. Heat Shock-Induced SGs Associate with Endoplasmic Reticulum and Mitochondria, and Their Contact Sites ERMES
2.5. Disassembly of Stress Granules after Heat Shock at 42 °C Is Also Hsp104-Dependent
2.6. Human TDP-43 Colocalizes with Heat Shock-Induced SGs in Yeast Cells and Facilitates Their Disassembly
3. Discussion
4. Materials and Methods
4.1. Strains and Growth Conditions
4.1.1. Galactose-Induction Conditions
4.1.2. Regrowth after Heat Shock
4.2. Construction of Plasmids and Yeast Strains
4.3. Western Blotting
4.4. Differential Centrifugation
4.5. Polysome Profile Analyses
4.6. Live-Cell Microscopy and Image Analysis
4.7. Image Analysis and Quantification
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Malcova, I.; Senohrabkova, L.; Novakova, L.; Hasek, J. eIF3a Destabilization and TDP-43 Alter Dynamics of Heat-Induced Stress Granules. Int. J. Mol. Sci. 2021, 22, 5164. https://doi.org/10.3390/ijms22105164
Malcova I, Senohrabkova L, Novakova L, Hasek J. eIF3a Destabilization and TDP-43 Alter Dynamics of Heat-Induced Stress Granules. International Journal of Molecular Sciences. 2021; 22(10):5164. https://doi.org/10.3390/ijms22105164
Chicago/Turabian StyleMalcova, Ivana, Lenka Senohrabkova, Lenka Novakova, and Jiri Hasek. 2021. "eIF3a Destabilization and TDP-43 Alter Dynamics of Heat-Induced Stress Granules" International Journal of Molecular Sciences 22, no. 10: 5164. https://doi.org/10.3390/ijms22105164